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  本文關(guān)鍵詞： 組織工程 梯度材料 聚己內(nèi)酯 胺解 骨髓間充質(zhì)干細(xì)胞 成骨分化　出處：《浙江大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：組織工程在修復(fù)受損組織、器官中有廣闊的應(yīng)用前景。界面組織工程注重不同組織間連接部分(界面)的修復(fù),突破了傳統(tǒng)組織工程中針對單一組織進行修復(fù)的限制,蘊含很多新的科學(xué)問題。我們希望通過構(gòu)建性質(zhì)梯度漸變的生物材料,并誘導(dǎo)骨髓間充質(zhì)干細(xì)胞(BMSCs)的定域分化以實現(xiàn)相鄰組織界面的修復(fù)。胺解是聚酯材料表面改性的常見方法,可用于構(gòu)建聚酯材料表面生物活性分子梯度以調(diào)控BMSCs的分化行為。但迄今有關(guān)胺解反應(yīng)動力學(xué)及其對材料性質(zhì)影響的研究尚不夠深入系統(tǒng),難以為梯度構(gòu)建提供科學(xué)依據(jù)與技術(shù)支持。本文首先系統(tǒng)研究了反應(yīng)條件對聚己內(nèi)酯(PCL)膜胺解的影響,在此基礎(chǔ)上梯度接枝了活性分子阿侖膦酸鈉(Aln),成功誘導(dǎo)了大鼠BMSCs的定域分化,取得了以下有價值的成果： (1)探明了小分子二胺分子量等反應(yīng)參數(shù)對胺解反應(yīng)的影響；闡明了1,6-己二胺濃度及反應(yīng)溫度與反應(yīng)速率的關(guān)系,以調(diào)控反應(yīng)速率和膜表面-NH2的密度。利用注射法梯度胺解了PCL膜,并通過戊二醛偶聯(lián)Aln,成功構(gòu)建了Aln線性密度梯度。 (2)發(fā)現(xiàn)大鼠BMSCs可正常粘附于不同Aln密度的PCL-Aln膜表面并進行增殖。通過與表面受體Cx43結(jié)合,Aln可促進BMSCs的成骨分化,其效應(yīng)與Aln密度大小正相關(guān)。梯度分布的Aln定域上調(diào)成骨相關(guān)蛋白堿性磷酸酶(ALP)、一型膠原(Col I)、成骨相關(guān)轉(zhuǎn)錄因子Runx2和骨鈣素(OCN)的表達(dá),顯示其成功誘導(dǎo)了BMSCs的定域分化。
[Abstract]:Tissue engineering has a broad application prospect in repairing damaged tissues and organs. The interface tissue engineering focuses on the repair of the joint part (interface) between different tissues. It breaks through the limitation of single tissue repair in traditional tissue engineering and contains many new scientific problems. We hope to construct biomaterials with gradient gradient. Local differentiation of bone marrow mesenchymal stem cells (BMSCs) was induced to repair adjacent tissue interfaces. Aminolysis is a common method for surface modification of polyester materials. It can be used to construct the molecular gradient of surface biological activity of polyester materials to regulate the differentiation behavior of BMSCs. However, the studies on the kinetics of amination reaction and its influence on the properties of polyester materials have not been thoroughly and systematically studied up to now. It is difficult to provide scientific basis and technical support for gradient construction. Firstly, the effect of reaction conditions on the amination of PCL membrane was studied systematically. On this basis, the active molecule Alendronate sodium alendronate was gradely grafted, and the local differentiation of rat BMSCs was induced successfully, and the following valuable results were obtained. 1) the influence of the reaction parameters such as the molecular weight of small molecule diamine on the amination reaction was investigated. The relationship between the concentration and temperature of 1-hexane diamine and the reaction rate was elucidated to regulate the reaction rate and the density of -NH _ 2 on the surface of the membrane. The PCL membrane was prepared by injection of gradient amine. The linear density gradient of Aln was successfully constructed by glutaraldehyde coupling. (2) it was found that rat BMSCs could adhere to and proliferate on the surface of PCL-Aln membrane with different Aln densities and bind to the surface receptor Cx43. Aln can promote the osteogenic differentiation of BMSCs, and its effect is positively related to the density of Aln. The gradient distribution of Aln upregulated the osteoblast-associated protein alkaline phosphatase (ALP). The expression of osteoblast-associated transcription factors (Runx2) and osteocalcin (OCN) in collagen I showed that they could induce the local differentiation of BMSCs successfully.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

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