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Analysis of Th1/Th2 Response Pattern for Erythrodermic Psori

發(fā)布時(shí)間:2021-01-09 00:15
  As one of the most serious types of psoriasis, pathogenesis of erythrodermic psoriasis(EP) is unclear so far. In this study, we aimed to detect the levels of Th1/Th2 cytokine-associated transcription factors and T-lymphocyte clone in peripheral blood mononuclear cells(PBMCs) derived from EP patients, and gene expression level of T-bet/GATA-3 in skin lesion. The potential role of Th1/Th2 reaction pattern played in the pathogenesis of EP was also discussed. Serum levels of IFN-γ, IL-2, IL-4 and IL... 

【文章來(lái)源】:Journal of Huazhong University of Science and Technology(Medical Sciences). 2014,34(04)

【文章頁(yè)數(shù)】:6 頁(yè)

【文章目錄】:
1 MATERIALS AND METHODS
    1.1 Sample Collection
    1.2 ELISA
    1.3 RNA Isolation, c DNA Synthesis and Real-time q PCR
    1.4 Flow Cytometry
    1.5 Statistical Analysis
2 RESULTS
    2.1 Serum Levels of IFN-γ, IL-2, IL-4 and IL-10
    2.2 Expression of T-bet and GATA-3 m RNA in Skin Lesions and PBMCs
    2.3 Correlation Analysis
    2.4 Comparison of Th1/Th2 Ratio in Peripheral Blo-od between Psoriasis Patients and Healthy Controls
3 DISCUSSION
Conflict of Interest Statement



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