【摘要】：目的 染發(fā)皮炎是臨床常見的變態(tài)反應(yīng)性疾病,對苯二胺是染發(fā)劑的主要功能成分,也是染發(fā)皮炎的主要致敏原之一。對苯二胺引起過敏的機制可能與環(huán)境因素、個體免疫狀態(tài)及易感性有關(guān)。通過對染發(fā)皮炎患者和正常對照組之間NAT1、NAr2及ACE等位基因及基因型的檢測,了解NAT1、NAT2及ACE各等位基因及基因型頻率分布,初步探索基因多態(tài)性與染發(fā)皮炎易感性之間的關(guān)系,探討染發(fā)皮炎的發(fā)病機制。 方法 收集2009年2月-2010年2月期間于天津醫(yī)科大學(xué)總醫(yī)院及天津市中醫(yī)藥研究院附屬醫(yī)院就診的染發(fā)皮炎患者60例和正常對照組73例的血樣,用TKM法提取DNA后,分別應(yīng)用聚合酶鏈式反應(yīng)擴增目的基因片段,擴增產(chǎn)物通過瓊脂糖凝膠電泳檢測,ACE基因直接根據(jù)瓊脂糖凝膠電泳結(jié)果判斷基因型；NAT1基因擴增產(chǎn)物經(jīng)Hinf1、AseⅠ、MboⅡ等限制性內(nèi)切酶作用后應(yīng)用聚丙烯酰胺凝膠電泳,NAT2基因擴增產(chǎn)物經(jīng)KpnⅠ、BamHⅠ、TaqⅠ等限制性內(nèi)切酶作用后,應(yīng)用瓊脂糖凝膠及聚丙烯酰胺凝膠電泳,根據(jù)電泳結(jié)果判斷各位點的核酸多態(tài)性,進而判斷基因型,總結(jié)得出染發(fā)皮炎組和對照組之間NAT1、NAT2及ACE各等位基因及各基因型的分布頻率,與國內(nèi)外人群中的分布頻率比較,并用卡方檢驗統(tǒng)計學(xué)方法分析比較各等位基因及基因型頻率在染發(fā)皮炎患者組和正常對照組的分布差異。 結(jié)果 1.NAT1基因：染發(fā)皮炎組NAT1*10等位基因頻率為35.8%,明顯低于對照組的頻率(χ2=3.954,P0.05)；NAT1基因型NAT1*4/*4,NAT1*4/*10, NAT1*10/*10,NAT1*3/*10,NAT1*3/*4在染發(fā)皮炎組的頻率分別是36.7%,40.0%,15.0%,1.7%,6.7%,在對照組為26.0%,42.5%,24.7%,4.1%,2.7%,兩組比較無統(tǒng)計學(xué)差異；病例組基因型中含有NAT1*10者的表型(快型)頻率為56.7%,略低于對照組的71.2%(X2=3.058,P=0.08)。 2.NAT2基因：NAT2等位基因NAT2*4,NAT2*5A,NAT2*6B和NAT2*7A在染發(fā)皮炎組中的分布頻率分別是52.5%,5.0%,26.7%,15.8%,在對照組中為55.5%,3.4%,27.4%,13.7%,兩組比較無統(tǒng)計學(xué)差異；快型、中間型、慢型基因型在病例組中頻率分別是26.7%,51.7%,21.7%,在對照組中為30.1%,50.7%,19.2%,兩組比較無統(tǒng)計學(xué)差異；快、慢乙酰化表型在病例組中的頻率分別是78.3%,21.7%,在對照組為80.8%,19.2%,兩組比較無統(tǒng)計學(xué)差異。 3.ACE基因：染發(fā)皮炎患者組I、D的頻率分別是55.8%,44.2%,對照組中為46.6%,53.4%,兩組比較無統(tǒng)計學(xué)差異；病例組中Ⅱ基因型頻率33.3%,明顯高于對照組的頻率(X2=4.255,P0.05)。結(jié)論 1.染發(fā)皮炎組NAT1*10等位基因頻率明顯低于對照組,含有NAT1*10者的乙�；硇�(快型)略低于對照組,兩組間基因型頻率無統(tǒng)計學(xué)差異。 2.NAT2基因的各等位基因頻率、基因型頻率及乙�；硇皖l率在染發(fā)皮炎組和正常對照組之間無統(tǒng)計學(xué)差異。 3. ACEⅡ基因型頻率在染發(fā)皮炎組顯著高于對照組,I、D等位基因頻率在兩組間無統(tǒng)計學(xué)差異。
[Abstract]:Objective hair dye dermatitis is a common allergic disease in clinic. P-phenylenediamine is the main functional component of hair dye and one of the main allergens of hair dye dermatitis. The mechanism of allergy induced by p-phenylenediamine may be related to environmental factors, individual immune status and susceptibility. The alleles and genotypes of NAT1,NAr2 and ACE were detected between the patients with hair dye dermatitis and the normal controls. The frequency distribution of NAT1,NAT2 and ACE alleles and genotypes were studied. To explore the relationship between gene polymorphism and hair dermatitis susceptibility, and to explore the pathogenesis of hair dye dermatitis. Methods from February 2009 to February 2010, 60 cases of hair dye dermatitis and 73 cases of normal control were collected from Tianjin Medical University General Hospital and affiliated Hospital of Tianjin Academy of traditional Chinese Medicine. DNA was extracted by TKM method. Polymerase chain reaction (PCR) was used to amplify the target gene fragments, and the amplified products were detected by agarose gel electrophoresis. The genotypes of ACE gene were judged directly by agarose gel electrophoresis. The products of NAT1 gene amplified by restriction endonuclease such as Hinf1,Ase 鈪，

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