miR-590-5p通過靶向抑制YAP1的表達(dá)抑制A375惡性黑素瘤細(xì)胞侵襲和遷移
發(fā)布時間：2018-12-15 23:33
【摘要】：目的探索miR-590-5p在A375惡性黑素瘤細(xì)胞中的表達(dá)及其對A375細(xì)胞侵襲和遷移能力的影響和機(jī)制。方法應(yīng)用實時定量PCR技術(shù)檢測A375細(xì)胞與人正常黑素細(xì)胞中miR-590-5p的表達(dá)水平;應(yīng)用TranswellTM實驗和劃痕實驗檢測miR-590-5p對A375細(xì)胞侵襲和遷移的影響;應(yīng)用熒光素酶報告基因?qū)嶒灻鞔_yes相關(guān)蛋白(YAP1)是否為miR-590-5p的直接靶分子;應(yīng)用實時定量PCR及Western blot法檢測miR-590-5p對YAP1表達(dá)水平的影響。結(jié)果 miR-590-5p在A375細(xì)胞中的表達(dá)水平顯著低于正常黑素細(xì)胞;與對照(miR-590-5p-NC)組相比,miR-590-5p mimcs組細(xì)胞侵襲和遷移能力均受到顯著抑制;YAP1為miR-590-5p的直接靶分子;與miR-590-5p-NC組相比,miR-590-5p mimcs組細(xì)胞中YAP1 mRNA及蛋白的表達(dá)均受到抑制。結(jié)論 miR-590-5p在A375細(xì)胞中低表達(dá),并通過靶向抑制YAP1的表達(dá)調(diào)控A375細(xì)胞的侵襲和遷移能力。
[Abstract]:Objective to investigate the expression of miR-590-5p in A375 malignant melanoma cells and its effect on the invasion and migration of A375 cells. Methods the expression of miR-590-5p in A375 cells and normal melanocytes was detected by real-time quantitative PCR, and the effect of miR-590-5p on the invasion and migration of A375 cells was detected by TranswellTM assay and scratch test. Luciferase reporter gene experiment was used to determine whether yes related protein (YAP1) was a direct target molecule of miR-590-5p, and real-time quantitative PCR and Western blot were used to detect the effect of miR-590-5p on YAP1 expression. Results the expression of miR-590-5p in A375 cells was significantly lower than that in normal melanocytes, and the ability of invasion and migration in miR-590-5p mimcs group was significantly inhibited compared with control group (miR-590-5p-NC). YAP1 was the direct target molecule of miR-590-5p, and the expression of YAP1 mRNA and protein in miR-590-5p mimcs group was inhibited compared with miR-590-5p-NC group. Conclusion the expression of miR-590-5p in A375 cells is low, and the ability of invasion and migration of A375 cells is regulated by targeting the expression of YAP1.
【作者單位】： 西安交通大學(xué)第一附屬醫(yī)院皮膚科;第四軍醫(yī)大學(xué)西京醫(yī)院泌尿外科;
【基金】：西安交通大學(xué)第一附屬醫(yī)院院基金(2016QN-06)
【分類號】：R739.5
[Abstract]:Objective to investigate the expression of miR-590-5p in A375 malignant melanoma cells and its effect on the invasion and migration of A375 cells. Methods the expression of miR-590-5p in A375 cells and normal melanocytes was detected by real-time quantitative PCR, and the effect of miR-590-5p on the invasion and migration of A375 cells was detected by TranswellTM assay and scratch test. Luciferase reporter gene experiment was used to determine whether yes related protein (YAP1) was a direct target molecule of miR-590-5p, and real-time quantitative PCR and Western blot were used to detect the effect of miR-590-5p on YAP1 expression. Results the expression of miR-590-5p in A375 cells was significantly lower than that in normal melanocytes, and the ability of invasion and migration in miR-590-5p mimcs group was significantly inhibited compared with control group (miR-590-5p-NC). YAP1 was the direct target molecule of miR-590-5p, and the expression of YAP1 mRNA and protein in miR-590-5p mimcs group was inhibited compared with miR-590-5p-NC group. Conclusion the expression of miR-590-5p in A375 cells is low, and the ability of invasion and migration of A375 cells is regulated by targeting the expression of YAP1.
【作者單位】： 西安交通大學(xué)第一附屬醫(yī)院皮膚科;第四軍醫(yī)大學(xué)西京醫(yī)院泌尿外科;
【基金】：西安交通大學(xué)第一附屬醫(yī)院院基金(2016QN-06)
【分類號】：R739.5
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 楊紅飛;鄭文宏;趙文淘;關(guān)春燕;安靚;;miR-590-5p和miR-590-3p參與肝細(xì)胞肝癌發(fā)展的機(jī)制[J];南方醫(yī)科大學(xué)學(xué)報;2013年06期
【共引文獻(xiàn)】
相關(guān)期刊論文 前9條
1 任舟輝;楊\，
本文編號：2381469
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