【摘要】：目的:明確新疆KS感染HHV-8的K14.1等位基因型,并初步探討新疆KS不同臨床分型及臨床表現(xiàn)與不同HHV-8亞型的相關性。方法:使用酚-氯仿-異戊醇法對新疆維吾爾自治區(qū)人民醫(yī)院收集的32例KS石蠟包埋組織進行HHV-8 DNA抽提,并使用巢式PCR擴增K14.1基因片段,確定K14.1等位基因型;最后運用Fisher確切概率法對結果進行統(tǒng)計學分析處理。結果:(1)32例新疆KS中有27例HHV-8感染為陽性,陽性率為84.38%,其中5例AIDS-KS患者HHV-8感染均為陽性,感染率100%;(2)基于K14.1位點,24例為P基因型,3例為M基因型;(3)不同HHV-8 K14.1等位基因型與新疆KS患者粘膜損害率之間的差異性無統(tǒng)計學意義(P0.05);(4)新疆經典型KS和AIDS-KS患者之間不同K14.1等位基因型感染率的差異性無統(tǒng)計學意義(P 0.05)。結論:(1)HHV-8感染在新疆KS的發(fā)生發(fā)展中起著不可忽視的作用;(2)新疆KS患者感染的HHV-8亞型主要屬于K14.1 P等位基因型,也存在部分的K14.1 M等位基因型感染者,其中K14.1等位基因型為國內首次報道;(3)新疆AIDS-KS患者趨向年輕化;(4) HHV-8 K14.1的不同基因型與新疆KS有無黏膜損害無關;(5)新疆KS患者感染的HHV-8 K14.1的不同基因型與KS臨床類型無關。
[Abstract]:Objective: to identify the K14.1 allele genotype of HHV-8 infected with KS in Xinjiang and to explore the correlation between different clinical types and clinical manifestations of KS and different HHV-8 subtypes in Xinjiang. Methods: using phenol-chloroform-isoamyl alcohol method, 32 cases of KS paraffin embedded tissues collected from Xinjiang Uygur Autonomous region people's Hospital were extracted by HHV-8 DNA. K14.1 gene fragment was amplified by nested PCR, and K14.1 allele genotype was determined. Finally, the Fisher exact probability method is used to analyze the results statistically. Results: (1) of 32 cases of KS in Xinjiang, 27 cases were positive for HHV-8 infection, the positive rate was 84.38%, among which 5 cases of AIDS-KS patients were all positive for HHV-8 infection, the infection rate was 100%; (2) based on K14.1 locus, 24 cases were P genotype, 3 cases were M genotype, (3) there was no significant difference between different HHV-8 K14.1 allele genotypes and mucosal damage rate of KS patients in Xinjiang (P0.05). (4) there was no significant difference in the infection rate of different K14.1 allele genotypes between Xinjiang classic KS and AIDS-KS patients (P0. 05). Conclusion: (1) HHV-8 infection plays an important role in the occurrence and development of KS in Xinjiang; (2) the HHV-8 subtypes of KS patients in Xinjiang were mainly K14.1P alleles, and there were some K14.1M alleles in Xinjiang. The K14.1 allele genotype was reported for the first time in China. (3) AIDS-KS patients in Xinjiang tend to be younger; (4) the different genotypes of HHV-8 K14.1 are not related to the mucosal damage of KS in Xinjiang; (5) the different genotypes of HHV-8 K14.1 infected by KS patients in Xinjiang are not related to the clinical types of KS.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R739.5

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