【摘要】：目的:明確新疆KS感染HHV-8的K14.1等位基因型,并初步探討新疆KS不同臨床分型及臨床表現(xiàn)與不同HHV-8亞型的相關(guān)性。方法:使用酚-氯仿-異戊醇法對(duì)新疆維吾爾自治區(qū)人民醫(yī)院收集的32例KS石蠟包埋組織進(jìn)行HHV-8 DNA抽提,并使用巢式PCR擴(kuò)增K14.1基因片段,確定K14.1等位基因型;最后運(yùn)用Fisher確切概率法對(duì)結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析處理。結(jié)果:(1)32例新疆KS中有27例HHV-8感染為陽性,陽性率為84.38%,其中5例AIDS-KS患者HHV-8感染均為陽性,感染率100%;(2)基于K14.1位點(diǎn),24例為P基因型,3例為M基因型;(3)不同HHV-8 K14.1等位基因型與新疆KS患者粘膜損害率之間的差異性無統(tǒng)計(jì)學(xué)意義(P0.05);(4)新疆經(jīng)典型KS和AIDS-KS患者之間不同K14.1等位基因型感染率的差異性無統(tǒng)計(jì)學(xué)意義(P 0.05)。結(jié)論:(1)HHV-8感染在新疆KS的發(fā)生發(fā)展中起著不可忽視的作用;(2)新疆KS患者感染的HHV-8亞型主要屬于K14.1 P等位基因型,也存在部分的K14.1 M等位基因型感染者,其中K14.1等位基因型為國內(nèi)首次報(bào)道;(3)新疆AIDS-KS患者趨向年輕化;(4) HHV-8 K14.1的不同基因型與新疆KS有無黏膜損害無關(guān);(5)新疆KS患者感染的HHV-8 K14.1的不同基因型與KS臨床類型無關(guān)。
[Abstract]:Objective: to identify the K14.1 allele genotype of HHV-8 infected with KS in Xinjiang and to explore the correlation between different clinical types and clinical manifestations of KS and different HHV-8 subtypes in Xinjiang. Methods: using phenol-chloroform-isoamyl alcohol method, 32 cases of KS paraffin embedded tissues collected from Xinjiang Uygur Autonomous region people's Hospital were extracted by HHV-8 DNA. K14.1 gene fragment was amplified by nested PCR, and K14.1 allele genotype was determined. Finally, the Fisher exact probability method is used to analyze the results statistically. Results: (1) of 32 cases of KS in Xinjiang, 27 cases were positive for HHV-8 infection, the positive rate was 84.38%, among which 5 cases of AIDS-KS patients were all positive for HHV-8 infection, the infection rate was 100%; (2) based on K14.1 locus, 24 cases were P genotype, 3 cases were M genotype, (3) there was no significant difference between different HHV-8 K14.1 allele genotypes and mucosal damage rate of KS patients in Xinjiang (P0.05). (4) there was no significant difference in the infection rate of different K14.1 allele genotypes between Xinjiang classic KS and AIDS-KS patients (P0. 05). Conclusion: (1) HHV-8 infection plays an important role in the occurrence and development of KS in Xinjiang; (2) the HHV-8 subtypes of KS patients in Xinjiang were mainly K14.1P alleles, and there were some K14.1M alleles in Xinjiang. The K14.1 allele genotype was reported for the first time in China. (3) AIDS-KS patients in Xinjiang tend to be younger; (4) the different genotypes of HHV-8 K14.1 are not related to the mucosal damage of KS in Xinjiang; (5) the different genotypes of HHV-8 K14.1 infected by KS patients in Xinjiang are not related to the clinical types of KS.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R739.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 盧春,曾怡,黃麗;HIV-1感染相關(guān)細(xì)胞因子激活BC-3細(xì)胞中潛伏感染的人類皰疹病毒8型的復(fù)制研究(英文)[J];Journal of Nanjing Medical University;2003年05期

2 普雄明;卡波西肉瘤；一種病譜性疾��？[J];皮膚病與性病;1996年01期

3 盧春,曾怡,錢超,唐桂霞;開放讀碼框架50基因介導(dǎo)HIV-1感染相關(guān)的肝細(xì)胞生長因子誘導(dǎo)人類皰疹病毒8型復(fù)制[J];中華傳染病雜志;2004年03期

4 康曉靜,沈大為,普雄明,吳衛(wèi)東,周炳華;新疆Kaposi肉瘤組織內(nèi)人類8型皰疹病毒DNA的檢測[J];中華皮膚科雜志;2001年06期

5 張德志;普雄明;吳衛(wèi)東;靳穎;;Kaposi肉瘤組織內(nèi)人類皰疹病毒8型的K15基因型研究[J];中華皮膚科雜志;2007年11期

6 杜文慧,陳國敏,孫荷,曾毅;新疆地區(qū)普通人群中人皰疹病毒8型IgG抗體的調(diào)查報(bào)告[J];中華實(shí)驗(yàn)和臨床病毒學(xué)雜志;2000年01期

7 盧春,曾怡,黃麗,錢超,唐桂霞;HIV-1感染相關(guān)細(xì)胞因子IFN-γ通過Rta基因啟動(dòng)子激活人類皰疹病毒8型[J];中華微生物學(xué)和免疫學(xué)雜志;2004年01期

8 盧春;唐桂霞;曾怡;錢超;秦娣;;HIV-1相關(guān)炎性細(xì)胞因子誘導(dǎo)人血管內(nèi)皮細(xì)胞中潛伏感染KSHV的溶解性周期復(fù)制[J];中華微生物學(xué)和免疫學(xué)雜志;2005年11期

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