【摘要】：目的:探討巨噬細(xì)胞能否被人乳頭瘤病毒感染。方法:我們?nèi)￠T診病人的尖銳濕疣組織制備成含HPV的混懸液,以及取健康人的外周血制備巨噬細(xì)胞,將兩者混合培養(yǎng),通過RT-PCR檢測巨噬細(xì)胞基因組中是否有HPV-DNA;電鏡觀察上述培養(yǎng)的巨噬細(xì)胞胞核內(nèi)是否含有HPV顆粒;免疫組化方法觀察在尖銳濕疣組織真皮乳頭層內(nèi)是否有CD68陽性細(xì)胞以及含HPV顆粒的陽性細(xì)胞;免疫熒光方法觀察真皮乳頭內(nèi)是否有對(duì)抗CD68熒光抗體和抗HPV熒光抗體均顯色的同一細(xì)胞。結(jié)果:1. RT-PCR方法檢測:含HPV溶液和巨噬細(xì)胞共培養(yǎng),四次傳代的后,巨噬細(xì)胞的基因組中均檢測到HPV6/11-DNA。2.電鏡觀察:上述傳代培養(yǎng)的巨噬細(xì)胞胞核中可見到HPV顆粒。3.免疫組化方法觀察:在尖銳濕疣組織真皮乳頭內(nèi)既有CD68陽性細(xì)胞,又有含HPV的陽性細(xì)胞。4.免疫熒光觀察:在尖銳濕疣組織真皮乳頭內(nèi)有顯示HPV的綠色熒光染,顯示CD68陽性細(xì)胞的橘色熒光,兩張圖片重疊后顯示橙黃色熒光。結(jié)論:尖銳濕疣組織內(nèi)的巨噬細(xì)胞能被HPV感染,這種被HPV感染的巨噬細(xì)胞的抗病毒功能很有可能被削弱。
[Abstract]:Objective: to investigate whether macrophages can be infected by human papillomavirus (HPV). Methods: we prepared suspension containing HPV from condyloma acuminatum tissue of outpatients and macrophages from peripheral blood of healthy people. The macrophages were mixed and cultured. RT-PCR was used to detect whether there was HPV-DNA; in the genome of macrophages. The HPV granules were observed by electron microscope in the nucleus of macrophages and whether there were CD68 positive cells and HPV positive cells in the dermal papillary layer of condyloma acuminatum tissue by immunohistochemical method. Immunofluorescence method was used to observe whether there were the same cells in dermal papilla that had both anti CD68 fluorescent antibody and anti HPV fluorescent antibody. The result is 1: 1. RT-PCR detection: HPV6/11-DNA.2. was detected in the genome of macrophages after four passages with HPV solution and macrophage co-cultured. Electron microscope observation: the above passage of macrophages can be seen in the nucleus of HPV particles. Immunohistochemical observation: there were CD68 positive cells and HPV positive cells in the dermal papilla of condyloma acuminatum. 4. 4. Immunofluorescence observation: in the dermal papilla of condyloma acuminatum there were green fluorescent staining of HPV and orange fluorescence of CD68 positive cells. Conclusion: macrophages in condyloma acuminatum tissue can be infected with HPV, and the antiviral function of macrophages infected with HPV is likely to be weakened.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R752.53

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