金雀異黃素對UVB氧化損傷HaCaT細胞的保護作用
發(fā)布時間：2018-10-31 06:51
【摘要】：目的研究金雀異黃素(genistein,Gen)對中波紫外線(UVB)照射人角質(zhì)形成細胞系HaCaT細胞所致氧化損傷的保護作用。方法體外培養(yǎng)HaCaT細胞,分成空白對照組(A組),25μmol/L金雀異黃素處理組(B組),UVB(30mJ/cm2)輻射組(C組),UVB輻射(30mJ/cm2)+25μmol/L金雀異黃素處理組(D組),MTT法檢測細胞增殖,流式細胞儀檢測活性氧(ROS)水平,生物化學法測定細胞超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-Px)活性。結(jié)果 D組與C組相比,細胞存活率明顯增加(C組62%,D組85%),細胞內(nèi)ROS水平明顯降低(C組237.21,D組120.57,抑制率為49%),細胞內(nèi)SOD和GSH-Px水平明顯升高[SOD:C組(180.55±8.34)nU/mL,D組(233.42±6.52)nU/mL;GSH-Px:C組(310.72±22.52)U,D組(380.98±29.50)U],兩組之間的差別均有顯著的統(tǒng)計學意義。結(jié)論 Gen能增加細胞在UVB照射后的存活率,增加細胞內(nèi)的抗氧化酶活性,降低細胞內(nèi)ROS水平,對UVB照射所致人角質(zhì)形成細胞氧化損傷具有保護作用。
[Abstract]:Objective to study the protective effect of genistein (genistein,Gen) on oxidative damage induced by ultraviolet B (UV) (UVB) irradiation on human keratinocytes HaCaT cells. Methods HaCaT cells were cultured in vitro and divided into two groups: blank control group (group A), 25 渭 mol/L genistein treatment group (group B,), UVB (30mJ/cm2) radiation group (group C,), UVB radiation), 25 渭 mol/L genistein treatment group (group D). Cell proliferation was detected by MTT, reactive oxygen (ROS) was detected by flow cytometry, and (SOD), glutathione peroxidase (GSH-Px) activity was measured by biochemical method. Results the cell survival rate in group D was significantly higher than that in group C (85% in group C and 62% in group D), and the level of intracellular ROS in group D was significantly decreased (group C, group 237.21, group D, 120.57, inhibition rate was 49%). Intracellular SOD and GSH-Px levels increased significantly [SOD:C group (180.55 鹵8.34) nU/mL,D group (233.42 鹵6.52) nU/mL;] GSH-Px:C group (310.72 鹵22.52) UD group (380.98 鹵29.50) U, there was significant difference between the two groups. Conclusion Gen can increase the survival rate of human keratinocytes after UVB irradiation, increase the activity of antioxidant enzymes, decrease the level of ROS in cells, and protect human keratinocytes from oxidative damage induced by UVB irradiation.
【作者單位】： 第三軍醫(yī)大學大坪醫(yī)院皮膚科;
【基金】：重慶市自然科學基金(CSTC.2010BB5021) 國家教育部留學歸國基金
【分類號】：R739.5
[Abstract]:Objective to study the protective effect of genistein (genistein,Gen) on oxidative damage induced by ultraviolet B (UV) (UVB) irradiation on human keratinocytes HaCaT cells. Methods HaCaT cells were cultured in vitro and divided into two groups: blank control group (group A), 25 渭 mol/L genistein treatment group (group B,), UVB (30mJ/cm2) radiation group (group C,), UVB radiation), 25 渭 mol/L genistein treatment group (group D). Cell proliferation was detected by MTT, reactive oxygen (ROS) was detected by flow cytometry, and (SOD), glutathione peroxidase (GSH-Px) activity was measured by biochemical method. Results the cell survival rate in group D was significantly higher than that in group C (85% in group C and 62% in group D), and the level of intracellular ROS in group D was significantly decreased (group C, group 237.21, group D, 120.57, inhibition rate was 49%). Intracellular SOD and GSH-Px levels increased significantly [SOD:C group (180.55 鹵8.34) nU/mL,D group (233.42 鹵6.52) nU/mL;] GSH-Px:C group (310.72 鹵22.52) UD group (380.98 鹵29.50) U, there was significant difference between the two groups. Conclusion Gen can increase the survival rate of human keratinocytes after UVB irradiation, increase the activity of antioxidant enzymes, decrease the level of ROS in cells, and protect human keratinocytes from oxidative damage induced by UVB irradiation.
【作者單位】： 第三軍醫(yī)大學大坪醫(yī)院皮膚科;
【基金】：重慶市自然科學基金(CSTC.2010BB5021) 國家教育部留學歸國基金
【分類號】：R739.5
【共引文獻】
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