【摘要】：目的檢測CD4+CD25+Foxp3+Treg細胞及細胞因子IL-10、IL-17、IL-23在慢性蕁麻疹患者外周血中的表達水平,探討CD4+CD25+Foxp3+Treg細胞、Th17細胞、IL-17/IL-23炎癥軸、IL-10之間的關(guān)系及在慢性蕁麻疹發(fā)病中的免疫學(xué)機制。方法對50例來自于青島大學(xué)附屬醫(yī)院黃島院區(qū)皮膚科門診的慢性蕁麻疹患者組收集病史資料,抽取外周血,對40例來自于青島大學(xué)附屬醫(yī)院黃島院區(qū)健康查體中心的健康對照組收集基本臨床信息并抽取外周血。采用流式細胞術(shù)檢測外周血中CD4+CD25+Foxp3+Treg細胞的表達水平,采用酶聯(lián)免疫吸附試驗(ELISA法)檢測血清中IL-10、IL-17、IL-23的表達水平。對兩組所測數(shù)據(jù)進行整理及統(tǒng)計學(xué)分析,設(shè)P0.05,差異有統(tǒng)計學(xué)意義。所得數(shù)據(jù)兩組間定量資料的比較采用t檢驗、組內(nèi)定量資料之間的相關(guān)性分析采用Pearson相關(guān)分析。結(jié)果(1)慢性蕁麻疹患者外周血中CD4+CD25+Foxp3+Treg細胞水平(1.59±0.50)%明顯低于健康對照組(3.24±0.77)%,差異有統(tǒng)計學(xué)意義(P0.05),且病程長者外周血Treg細胞水平(1.18±0.33)%低于病程較短者(1.79±0.29)%,差異有統(tǒng)計學(xué)意義(P0.05)。(2)慢性蕁麻疹患者血清中IL-10水平(199.09±149.99)pg/ml略高于健康對照組(162.79±134.44)pg/ml,差異無統(tǒng)計學(xué)意義(P0.05)。(3)慢性蕁麻疹患者血清中IL-17水平(294.09±76.97)pg/ml明顯高于健康對照組(104.71±43.58)pg/ml,差異有統(tǒng)計學(xué)意義(P0.05)。(4)慢性蕁麻疹患者血清中IL-23水平(2189.13±612.49)pg/ml明顯高于健康對照組(962.20±383.92)pg/ml,差異有統(tǒng)計學(xué)意義(P0.05)。(5)慢性蕁麻疹患者血清中IL-17水平與IL-23水平呈正相關(guān)(r=0.439,P0.05)。(6)慢性蕁麻疹患者外周血中CD4+CD25+Foxp3+Treg細胞水平與IL-10水平無明顯相關(guān)性(r=-0.263,P0.05)。(7)慢性蕁麻疹患者外周血中CD4+CD25+Foxp3+Treg細胞水平與IL-17水平無明顯相關(guān)性(r=0.264,P0.05)。結(jié)論慢性蕁麻疹患者外周血中CD4+CD25+Foxp3+Treg細胞比例及IL-17、IL-23水平存在明顯異常,提示CD4+CD25+Foxp3+Treg細胞和IL-17、IL-23參與了慢性蕁麻疹的發(fā)病,推測慢性蕁麻疹的發(fā)生可能與自身免疫有關(guān),需要進一步研究。意義通過該課題的研究,可以為慢性蕁麻疹的預(yù)防及治療提供實驗室依據(jù),也可以為其它過敏性皮膚病的研究及治療提供新的思路和方法。
[Abstract]:Objective to detect the expression of CD4 CD25 Foxp3 Treg cells and cytokines IL-10,IL-17,IL-23 in peripheral blood of patients with chronic urticaria and to explore the inflammatory axis of CD4 CD25 Foxp3 Treg cells, Th17 cells and IL-17/IL-23. The relationship between IL-10 and the immunological mechanism in the pathogenesis of chronic urticaria. Methods 50 patients with chronic urticaria from the Department of Dermatology, Huangdao Hospital, Qingdao University, were collected and their peripheral blood was drawn. The basic clinical information and peripheral blood were collected from 40 healthy controls from Huangdao District Health examination Center of affiliated Hospital of Qingdao University. The expression of CD4 CD25 Foxp3 Treg cells in peripheral blood was detected by flow cytometry and the expression of IL-10,IL-17,IL-23 in serum was detected by enzyme linked immunosorbent assay (ELISA). The data of the two groups were collected and statistically analyzed, and the difference was statistically significant (P 0.05). T test was used to compare the quantitative data between the two groups, and Pearson correlation analysis was used to analyze the correlation between the quantitative data of the two groups. Results (1) the level of CD4 CD25 Foxp3 Treg cells in peripheral blood of patients with chronic urticaria (1.59 鹵0.50)% was significantly lower than that of healthy controls (3.24 鹵0.77)%, the difference was statistically significant (P0.05). The level of Treg cells in peripheral blood of elderly patients with disease course (1.18 鹵0.33)% was lower than that of those with shorter course (1.79 鹵0.29)%. The serum IL-10 level of patients with chronic urticaria (199.09 鹵149.99) pg/ml was slightly higher than that of healthy controls (162.79 鹵134.44) pg/ml, (P0.05). (2). There was no significant difference (P0.05). (3) in serum IL-17 level between patients with chronic urticaria (294.09 鹵76.97) pg/ml and healthy control group (104.71 鹵43.58 pg/ml,). The serum IL-23 level of patients with chronic urticaria (2189.13 鹵612.49) pg/ml was significantly higher than that of healthy controls (962.20 鹵383.92) pg/ml, (P0.05). (4). There was a positive correlation between serum IL-17 level and IL-23 level in patients with chronic urticaria (P0.05). (5). P0.05). (6) there was no significant correlation between CD4 CD25 Foxp3 Treg cell level and IL-10 level in peripheral blood of patients with chronic urticaria (r = 0.263, P < 0.05). There was no significant correlation between CD4 CD25 Foxp3 Treg cell level and IL-17 level in peripheral blood of patients with chronic urticaria (P 0.05). Conclusion the proportion of CD4 CD25 Foxp3 Treg cells and the level of IL-17,IL-23 in peripheral blood of patients with chronic urticaria are obviously abnormal, which suggests that CD4 CD25 Foxp3 Treg cells and IL-17,IL-23 are involved in the pathogenesis of chronic urticaria. It is speculated that the occurrence of chronic urticaria may be related to autoimmunity, which needs further study. The purpose of this study is to provide laboratory basis for the prevention and treatment of chronic urticaria, and to provide new ideas and methods for the study and treatment of other allergic dermatosis.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R758.24

【參考文獻】

相關(guān)期刊論文 前10條

1 齊婧;王杰穎;連石;張海萍;;慢性蕁麻疹患者的甲狀腺功能及自身抗體水平分析[J];中國麻風(fēng)皮膚病雜志;2016年12期

2 陳佰超;李巍;;蕁麻疹的非IgE介導(dǎo)機制[J];國際皮膚性病學(xué)雜志;2016年03期

3 張飛娟;白莉;;慢性蕁麻疹患者外周血中白細胞介素25、17的表達及意義[J];中國中西醫(yī)結(jié)合皮膚性病學(xué)雜志;2016年01期

4 郭敏;白莉;;慢性蕁麻疹患者血清IL-35和IL-17的檢測[J];中國麻風(fēng)皮膚病雜志;2016年02期

5 劉金花;李慶祥;陳俊釗;黃文富;曾美;王翠彥;;慢性蕁麻疹兒童血清IFN-γ、IL-10水平檢測[J];現(xiàn)代診斷與治療;2013年06期

6 李潤祥;梁碧華;楊婧;熊斯穎;馬少吟;梁艷華;朱慧蘭;;慢性自發(fā)性蕁麻疹患者血清IL-17、IL-23水平的檢測及意義[J];皮膚性病診療學(xué)雜志;2013年01期

7 楊婧;朱慧蘭;;Th17細胞/Treg細胞及其失衡在慢性自發(fā)性蕁麻疹發(fā)病機制中的作用[J];國際皮膚性病學(xué)雜志;2012年06期

8 門守保;;慢性特發(fā)性蕁麻疹患者血清IL-2、4、6、8、10、12及IFN-γ的檢測及臨床意義[J];皮膚病與性病;2011年05期

9 馮歡;郭胤仕;;慢性蕁麻疹患者外周血CD4~+CD25~+調(diào)節(jié)性T細胞的表達[J];臨床皮膚科雜志;2011年03期

10 沈斌;冷建杭;王克義;吳黎明;卜璋于;李雅芬;陳健;蘇明;;慢性特發(fā)性蕁麻疹患者外周血調(diào)節(jié)性T細胞和IL-17水平的研究[J];中華皮膚科雜志;2010年12期

，

本文編號：2298347