【摘要】：目的探討初發(fā)和復(fù)發(fā)尖銳濕疣(CA)患者皮損中TLR4、TLR9和DC-SIGN的表達及其臨床病理意義。 方法收集正常人皮膚（包皮）20例、初發(fā)CA組織30例、復(fù)發(fā)CA組織30例，，采用免疫組織化學(xué)鏈霉抗生物素蛋白－過氧化物酶法（SP法）檢測以上組織中TLR4、TLR9和DC-SIGN的表達及分布。參照Fromowitz評分標(biāo)準(zhǔn)得出每個標(biāo)本結(jié)果，應(yīng)用SPSS17．0統(tǒng)計軟件以t檢驗法比較TLR4、TLR9和DC-SIGN在初發(fā)CA皮損與復(fù)發(fā)CA皮損中的差異，Spearman等級相關(guān)分析TLR4、TLR9和DC-SIGN在初發(fā)CA皮損與復(fù)發(fā)CA皮損中的的相關(guān)性。 結(jié)果TLR4、TLR9和DC-SIGN在初發(fā)CA皮損和復(fù)發(fā)CA皮損中的陽性率均為100%，在正常包皮組織中陽性率分別為5%、10%、5%。初發(fā)CA皮損和復(fù)發(fā)CA皮損中TLR4、TLR9和DC-SIGN的表達水平均顯著高于正常包皮組織（P0.001），且陽性細胞集中分布于基底層和棘層。TLR4、TLR9和DC-SIGN三者在初發(fā)CA皮損和復(fù)發(fā)CA皮損中的表達差異均無統(tǒng)計學(xué)意義（三者均P0.05）。TLR4、TLR9與DC-SIGN在初發(fā)CA皮損和復(fù)發(fā)CA皮損中的表達均呈正相關(guān)性關(guān)系(rs1=0.489，P0.05;rs2=0.475，P0.05)。 結(jié)論TLR4、TLR9和DC-SIGN在初發(fā)CA皮損和復(fù)發(fā)CA皮損中的表達明顯增強可能在CA的發(fā)病和復(fù)發(fā)過程中起到一定作用。
[Abstract]:Objective to investigate the expression and clinicopathological significance of TLR4,TLR9 and DC-SIGN in the lesions of patients with primary and recurrent condyloma acuminatum (CA). Methods 20 cases of normal skin (prepuce), 30 cases of primary CA and 30 cases of recurrent CA were collected. The expression and distribution of TLR4,TLR9 and DC-SIGN in the tissues were detected by immunohistochemical streptavidin-peroxidase (SP) method. The results of each specimen were obtained by reference to the Fromowitz score, SPSS17.0 statistical software was used to compare the difference between TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions. Spearman grade correlation was used to analyze the correlation between TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions. Results the positive rates of TLR4,TLR9 and DC-SIGN in the primary CA lesions and recurrent CA lesions were 100, and in the normal prepuce tissues, the positive rates were 51010 and 555, respectively. The expression levels of TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions were significantly higher than those in normal prepuce tissues (P0.001), and the positive cells were concentrated in the basal layer and spinous layer. The expression of TLR4,TLR9 and DC-SIGN in the primary CA lesions and recurrent CA lesions was poor. The expression of TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions were positively correlated (rs1=0.489,P0.05;rs2=0.475,P0.05). Conclusion the increased expression of TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions may play a role in the pathogenesis and recurrence of CA.
【學(xué)位授予單位】：廣州醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R752.53

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