【摘要】：背景和目的 尖銳濕疣(condylomata acuminatum,CA)是性傳播疾病(sexually transmitted disease,STD)的一種,由人類乳頭瘤病毒(human papillomavirus,HPV)感染生殖器、會陰和肛門等部位引起的一種常見的性傳播疾病。HPV是雙鏈DNA病毒,在鱗狀上皮復(fù)制,通過與受損的皮膚接觸進行傳播。近年來,其發(fā)生率迅速增加,據(jù)我國性病檢測報道,生殖器HPV檢測率為30.69/10萬,占我國性傳播疾病的第二位；在國外,其發(fā)病率僅次于淋病,研究其發(fā)病機制對控制CA具有十分重要的意義。本實驗通過對P-STAT3和COX-2在CA組織中的表達(dá)及其相關(guān)性的研究探討尖銳濕疣的發(fā)病機制,為其預(yù)防和治療提供理論依據(jù)。 尖銳濕疣具有增生快、易復(fù)發(fā)的特點,少數(shù)患者過度增生成為巨大型尖銳濕疣,部分可發(fā)生腫瘤。近年來研究發(fā)現(xiàn)在人體鱗狀上皮細(xì)胞感染HPV后存在細(xì)胞凋亡和增殖異常,關(guān)于其機制的研究較多,但P-STAT3和COX-2在尖銳濕疣及生殖器癌的發(fā)病機制研究較少,本實驗我們用免疫組織化學(xué)SP法分別檢測43例CA組織和32例正常人包皮組織中P-STAT3和COX-2的表達(dá),并分析CA組織中P-STAT3和COX-2表達(dá)的相關(guān)性。 材料和方法 1材料 43例CA患者均來自鄭州大學(xué)第一附屬醫(yī)院皮膚性病科門診,具有典型皮損,并經(jīng)組織病理證實,其中男31例,女12例；年齡17-55(31.42±7.28)歲；病程20-108(54.7±13.63)天；以32例由泌尿外科手術(shù)切除的正常男性包皮作為對照。 2方法 應(yīng)用免疫組化SP法分別檢測43例CA組織和32例正常人包皮組織中p-STAT3和COX-2的表達(dá),操作步驟均按試劑盒說明進行。P-STAT3陽性顯色為細(xì)胞核內(nèi)出現(xiàn)淡黃色至棕褐色顆粒；COX-2陽性顯色為細(xì)胞膜和胞膜附近的胞質(zhì)出現(xiàn)淡黃色至棕褐色顆粒。 3統(tǒng)計學(xué)處理 采用SPSS17.0進行分析,應(yīng)用兩獨立樣本秩和檢驗比較兩組組織中P-STAT3和COX-2蛋白表達(dá)水平的差異,應(yīng)用x2檢驗比較兩組陽性表達(dá)率差異,應(yīng)用Pearson列聯(lián)系數(shù)方法分析CA組織中兩種蛋白表達(dá)的關(guān)聯(lián)性,檢驗水準(zhǔn)a=0.05. 結(jié)果 1CA組織和正常上皮組織中P-STAT3蛋白的表達(dá) P-STAT3陽性顯色為細(xì)胞核內(nèi)出現(xiàn)淡黃色至棕褐色顆粒。43例CA組織中有28例P-STAT3陽性表達(dá),陽性表達(dá)率65.12%(28/43),表達(dá)強度多在++~+++,P-STAT3陽性細(xì)胞主要位于棘細(xì)胞層和顆粒層角質(zhì)形成細(xì)胞的胞核中,正常上皮組織中無表達(dá)。兩組陽性表達(dá)率差異有統(tǒng)計學(xué)意義(x2=30.53,P0.05),兩組陽性表達(dá)強度差異有統(tǒng)計學(xué)意義(Z=5.575,P0.001)。 2CA組織和正常上皮組織中COX-2蛋白的表達(dá) COX-2陽性顯色為細(xì)胞膜和胞膜附近的胞質(zhì)出現(xiàn)淡黃色至棕褐色顆粒。43例CA組織中有32例COX-2陽性表達(dá),陽性表達(dá)率74.42%(32/43),表達(dá)強度多在++～+++,陽性細(xì)胞主要位于棘層的中上部和顆粒層。正常上皮組織中COX-2僅有少量表達(dá),陽性表達(dá)率6.25%(2/32),表達(dá)強度在-~++,兩組陽性表達(dá)率差異有統(tǒng)計學(xué)意義(x2=31.71,P0.05),兩組陽性表達(dá)強度差異有統(tǒng)計學(xué)意義(Z=5.713,P0.001)。 3CA組織中p-STAT3和COX-2表達(dá)的關(guān)聯(lián)性分析 在43例CA組織中p-STAT3表達(dá)陽性的有28例,15例陰性；有32例COX-2表達(dá)陽性,11例陰性。經(jīng)Pearson相關(guān)分析后發(fā)現(xiàn),43例CA組織中p-STAT3與COX-2表達(dá)呈正相關(guān)(r=0.577，P0.001)。 結(jié)論 1.CA組織中存在p-STAT3的過表達(dá),p-STAT3的過表達(dá)可能與CA組織細(xì)胞的過度增殖有關(guān)； 2.CA組織中存在COX-2的過表達(dá),提示COX-2對CA的發(fā)生發(fā)展起重要作用； 3.在CA組織中p-STAT3與COX-2的表達(dá)呈正相關(guān),二者的共同作用促進CA的發(fā)生和發(fā)展。
[Abstract]:Background and purpose
Condylomata acuminatum (CA) is a sexually transmitted disease (STD), caused by human papillomavirus (HPV) infection of genitals, perineum and anus and other parts of a common sexually transmitted disease. Contact transmission. In recent years, the incidence of STD has increased rapidly. According to the reports of STD detection in China, the detection rate of HPV in genitals is 3069/100,000, accounting for the second place of STD in China. In foreign countries, the incidence of HPV is second only to gonorrhea, so it is very important to study its pathogenesis for controlling CA. To explore the pathogenesis of condyloma acuminatum and provide theoretical basis for its prevention and treatment.
Condyloma acuminatum (CA) has the characteristics of rapid proliferation and easy recurrence. Excessive hyperplasia in a few patients has become a giant CA, and some of them can develop tumors. In recent years, studies have found that there are abnormal apoptosis and proliferation in human squamous epithelial cells infected with HPV. There are many studies on its mechanism, but P-STAT3 and COX-2 in CA and genital cancer. In this study, we detected the expression of P-STAT3 and COX-2 in 43 CA tissues and 32 normal prepuce tissues by immunohistochemical SP method, and analyzed the correlation between the expression of P-STAT3 and COX-2 in CA tissues.
Materials and methods
1 material
43 patients with CA were all from the dermatology and venereal disease clinic of the First Affiliated Hospital of Zhengzhou University. The typical lesions were confirmed by histopathology, including 31 males and 12 females, aged 17-55 (31.42 (+ 7.28) years, 20-108 (54.7 (+ 13.63) days, and 32 normal male prepuce excised by urological surgery as control.
2 method
The expression of p-STAT3 and COX-2 in 43 CA tissues and 32 normal prepuce tissues were detected by immunohistochemical SP method. The operation procedure was carried out according to the kit instructions. Grain.
3 statistical processing
SPSS17.0 was used to analyze the expression of P-STAT3 and COX-2 protein in CA tissues. The expression of P-STAT3 and COX-2 protein in CA tissues was compared by rank sum test of two independent samples. The positive expression rate of P-STAT3 and COX-2 protein in CA tissues was compared by x2 test.
Result
Expression of P-STAT3 protein in 1CA and normal epithelial tissue
The positive staining of P-STAT3 was pale yellow to brown granules in the nucleus. 28 of 43 CA tissues were positive for P-STAT3. The positive expression rate was 65.12%(28/43). The expression intensity of P-STAT3 was mostly +++. The P-STAT3 positive cells were mainly located in the nuclei of keratinocytes in the spinous cell layer and granular layer, but not in the normal epithelium. The positive expression intensity of the two groups was significantly different (Z = 5.575, P 0.001).
Expression of COX-2 protein in 2CA and normal epithelial tissue
The positive staining of COX-2 was pale yellow to brown granules in the cytoplasm near the cell membrane and membrane. In 43 CA tissues, 32 cases were positive for COX-2, the positive expression rate was 74.42%(32/43). The expression intensity of COX-2 was mostly +++, and the positive cells were mainly located in the middle and upper part of the spinous layer and granular layer. The positive rate was 6.25% (2/32) and the expression intensity was -++. The difference was statistically significant between the two groups (x2 = 31.71, P 0.05). The difference was statistically significant between the two groups (Z = 5.713, P 0.001).
Correlation analysis of p-STAT3 and COX-2 expression in 3CA tissues
There were 28 positive and 15 negative cases of p-STAT3 expression in 43 CA tissues, 32 positive and 11 negative cases of COX-2 expression. Pearson correlation analysis showed that p-STAT3 expression was positively correlated with COX-2 expression in 43 CA tissues (r=0.577, P 0.001).
conclusion
1. The overexpression of p-STAT3 in CA tissues may be related to the overproliferation of CA cells.
The over expression of COX-2 in 2.CA tissues suggests that COX-2 plays an important role in the development of CA.
3. The expression of p-STAT3 and COX-2 was positively correlated in CA tissues. The co-action of p-STAT3 and COX-2 promoted the occurrence and development of CA.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R752.53

【參考文獻】

相關(guān)期刊論文 前10條

1 劉俊茹;王原;左連富;李鳳林;王瑩;劉嘉琳;;COX-2、p-Stat3及p-Stat5在食管癌組織中的表達(dá)及其意義[J];癌癥;2007年05期

2 王芳;王翠蓮;郭麗麗;王志萍;;COX-2 Survivin在大腸癌組織中的表達(dá)及意義[J];長治醫(yī)學(xué)院學(xué)報;2010年01期

3 李艷麗;楊麗麗;鄭紅兵;;COX-2在宮頸病變中的表達(dá)及其與高危型HPV感染的相關(guān)性[J];當(dāng)代醫(yī)學(xué);2010年33期

4 郭華陽，周來新，劉斌，鐘白玉，葉慶佾，譚駿，劉昕;復(fù)發(fā)尖銳濕疣中人乳頭瘤病毒基因的原位雜交法分型觀察[J];第三軍醫(yī)大學(xué)學(xué)報;1996年01期

5 劉彤;張洪輝;;尖銳濕疣患者病損中VEGF、COX-2及MVD的作用探討[J];四川大學(xué)學(xué)報(醫(yī)學(xué)版);2009年02期

6 佘曉玲;劉保安;;Stat3與腫瘤的侵襲轉(zhuǎn)移[J];現(xiàn)代生物醫(yī)學(xué)進展;2007年02期

7 郭穎;李海;陳殷;陳芳;張陽;;環(huán)氧化酶-2和血管內(nèi)皮生長因子在尖銳濕疣組織中的表達(dá)及臨床病理意義[J];實用醫(yī)技雜志;2006年21期

8 王海清;王海琳;;磷酸化轉(zhuǎn)錄信號轉(zhuǎn)導(dǎo)子及激活子3和細(xì)胞周期素D1的表達(dá)與宮頸癌臨床病理特點的關(guān)系[J];實用醫(yī)學(xué)雜志;2008年09期

9 冉鳳明;魏萬里;胡紅艷;王小芳;;STAT3、p-STAT3、HIF-1α、VEGF蛋白表達(dá)與肺癌侵襲和轉(zhuǎn)移的相關(guān)性研究[J];實用醫(yī)學(xué)雜志;2011年14期

10 馮松濤;張志剛;;人乳頭瘤病毒致癌機制的研究進展[J];國際病理科學(xué)與臨床雜志;2010年01期

相關(guān)碩士學(xué)位論文 前1條

1 劉新;TRX與STAT-3在肝細(xì)胞肝癌高表達(dá)及相互作用研究[D];浙江大學(xué);2007年

，

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