【摘要】：目的探討苯烯莫德對(duì)人外周血單個(gè)核細(xì)胞(PBMC)增殖凋亡及T細(xì)胞核因子NF-κB的調(diào)節(jié)作用。方法提取健康志愿者外周血,密度梯度離心法分離單個(gè)核細(xì)胞,用CCK-8法測(cè)定苯烯莫德對(duì)人PBMC增殖的影響;用流式細(xì)胞法測(cè)定苯烯莫德對(duì)人PBMC凋亡的影響;用Western blot法檢測(cè)活化的人T細(xì)胞核因子NF-κB p65,p-NF-κb p65,IKK,p-IKK,IκBα,p-IκBα蛋白表達(dá)的經(jīng)時(shí)變化。結(jié)果苯烯莫德抑制人PBMC增殖的IC50為9.67μmol/L;苯烯莫德可誘導(dǎo)人PBMC的早期凋亡和晚期凋亡;苯烯莫德作用于活化的T細(xì)胞后,隨著時(shí)間的延長,NF-κB通路中活化性因子p-NF-κB p65和p-IKK表達(dá)逐漸降低,而抑制性因子p-IκBα表達(dá)卻逐漸升高,提示苯烯莫德對(duì)NF-κB通路為負(fù)向調(diào)節(jié)。結(jié)論苯烯莫德抑制人PBMC增殖,誘導(dǎo)其凋亡。苯烯莫德對(duì)T細(xì)胞NF-κB通路為負(fù)向調(diào)節(jié)。
[Abstract]:Objective to investigate the effect of benimod on proliferation and apoptosis of human peripheral blood mononuclear cells (PBMC) and regulation of T cell nuclear factor NF- 魏 B. Methods Mononuclear cells were isolated from healthy volunteers by density gradient centrifugation. The effects of benimod on the proliferation of human PBMC and apoptosis of human PBMC were determined by CCK-8 assay and flow cytometry respectively. The expression of activated human T cell nuclear factor NF- 魏 B p65, p-NF- 魏 B and I 魏 B 偽-I 魏 B 偽 was detected by Western blot assay, and the expression of p-I 魏 B 偽 -p-I 魏 B 偽 was detected. Results Benimod inhibited the proliferation of human PBMC with IC50 of 9.67 渭 mol/L;, which could induce early and late apoptosis of human PBMC. The expression of activating factor p-NF- 魏 B p65 and p-IKK in NF- 魏 B pathway decreased gradually with time, while the expression of inhibitory factor p-I 魏 B 偽 increased gradually, suggesting that benzimod has negative regulation on NF- 魏 B pathway. Conclusion Benimod inhibits the proliferation of human PBMC and induces its apoptosis. Benimod negatively regulated the NF- 魏 B pathway in T cells.
【作者單位】： 北京大學(xué)人民醫(yī)院皮膚科;
【分類號(hào)】：R751
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本文編號(hào)：2231985