【摘要】：目的： 檢測脂溢性角化病、光化性角化病、Bowen病及皮膚鱗狀細胞癌患者皮損組織中DKK1和β-catenin的表達情況，探討DKK1和β-catenin在不同日光相關(guān)性皮膚腫瘤中的表達意義及其相關(guān)性。 方法： 1.收集在我院皮膚科病理室經(jīng)臨床和病理確診的脂溢性角化病19例、光化性角化病16例、Bowen病24例及皮膚鱗狀細胞癌25例。另外選取22例正常表皮組織標本作為對照，應(yīng)用免疫組織化學SABC方法檢測組織中DKK1和β-catenin的表達。 2.對DKK1和β-catenin的表達進行相關(guān)性分析。 結(jié)果： 1．DKK1在正常表皮組織的陽性表達定位于胞質(zhì)中，在SK、AK、BD和SCC中的陽性表達率分別為63.16%、50.00%、12.50%、8.00%。 2.β-catenin在正常表皮組織中的表達定位于胞膜，在SK、AK、BD和SCC中的異常表達率分別為15.79%、56.25%、91.67%、96.00%。 3.皮膚鱗癌中DKK1和β-catenin的表達存在負相關(guān)(r=-0.692,P=0.000)。 結(jié)論： 1.在表皮惡性轉(zhuǎn)化的過程中，隨著病變惡性程度的增加，DKK1的陽性表達率逐漸下降，而β-catenin的異常表達率逐漸上升，提示W(wǎng)nt信號通路可能在皮膚鱗癌的發(fā)生過程中發(fā)揮了一定作用。 2.皮膚鱗狀細胞癌中DKK1和β-catenin的表達存在負相關(guān)，，提示DKK1表達的缺失可能通過作用于經(jīng)典Wnt信號傳導(dǎo)通路促進β-catenin的異常表達。
[Abstract]:Objective: to detect the expression of DKK1 and 尾 -catenin in the lesions of seborrheic keratosis, actinic keratosis and squamous cell carcinoma. To investigate the expression and correlation of DKK1 and 尾-catenin in different sun-associated skin tumors. Methods: 1. Nineteen cases of seborrheic keratosis, 24 cases of photokeratosis and 25 cases of squamous cell carcinoma of skin were collected. The expression of DKK1 and 尾 -catenin in 22 normal epidermal tissues were detected by immunohistochemical SABC method. 2. The correlation between the expression of DKK1 and 尾-catenin was analyzed. Results: the positive expression of 1.DKK1 in normal epidermis was localized in cytoplasm, and the positive expression rate in SKK AKB BD and SCC was 63.1650.The positive expression rate of 尾 -catenin in normal epidermis was 8.000.The expression of 尾 -catenin in normal epidermis was located in the membrane. The abnormal expression rates in SKK AKB BD and SCC were 15.79 and 56.25, 91.67 and 96.00, respectively. There was a negative correlation between the expression of DKK1 and 尾 -catenin in squamous cell carcinoma of skin. Conclusion: 1. In the process of malignant transformation of epidermis, the positive rate of DKK1 decreased gradually with the increase of malignant degree of lesions, while the abnormal expression rate of 尾 -catenin increased gradually. The results suggest that Wnt signaling pathway may play a role in the development of cutaneous squamous cell carcinoma. 2. 2. There is a negative correlation between the expression of DKK1 and 尾 -catenin in squamous cell carcinoma of skin, suggesting that the absence of DKK1 expression may promote the abnormal expression of 尾 -catenin by acting on the classical Wnt signaling pathway.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R739.5

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相關(guān)期刊論文 前1條

1 高天文,孫東杰,李春英,何弘,李青,劉友生,刁慶春,黃高升,郝飛,鐘白玉,馬福成,柳鳳軒,閆小初,劉東梅,劉玉峰,劉榮卿;中國西部兩醫(yī)院1905例皮膚惡性腫瘤回顧分析[J];北京大學學報(醫(yī)學版);2004年05期

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