尋常型銀屑病患者血清IL-17、IL-22水平的表達(dá)
[Abstract]:Objective: psoriasis (psoriasis) is a common and recurring chronic inflammatory proliferative dermatosis. It is mainly characterized by inflammatory red papules in the scalp, trunk, and limb extensor distribution. The plaque and the surface are covered with multi-layered and dry silver white scales. There are thin film phenomena and punctate bleeding, and most of the skin lesions are in the skin. It is light in winter and light in summer and high in recurrence rate, which seriously affects the physical and mental health and quality of life of the patients. The basic pathological manifestations are hyperkeratinization and keratinization of epidermal keratinocytes, which are usually divided into ordinary, pustular, arthrosis and erythroderma, and the most common type. The pathogenesis is not completely clear. Most of the previous studies suggest that psoriasis is a kind of T lymphocyte dysfunction and the autoimmune disease with Th1 cell overactivation as the predominance of [14]. has been found in recent years in the study of the specific response to autoimmune diseases and allergens. The adjunctive T cells, which produce interleukin 17, are called Th17 cells. The cells secrete IL-22, IL-21 and other cytokines in addition to the main secretion of the specific effector molecule IL-17. From the discovery of Th17 cells, people begin to re understand and evaluate the regulatory role of Th1 cells in autoimmune diseases, more related to Th17 cells and their effects. The research of cytokines in autoimmune diseases has become a hot topic.
In this experiment, the expression level of interleukin -17 (interleukin 17, IL-17), interleukin -22 (interleukin 22, IL-22) in the serum of patients with psoriasis vulgaris was detected and compared with that of the healthy control group. The correlation between the expression level of IL-17 and IL-22 in serum and the pathogenesis of psoriasis vulgaris and the effect on the progression of the disease were further studied. Objective to investigate the role of Th17 cell and its effector factor IL-17 and IL-22 in the pathogenesis of psoriasis.
Methods: 29 cases of psoriasis vulgaris in the Department of Dermatology of the second hospital of Hebei Medical University from June 2010 to November 2010 were selected as the case group. The standard of admission: 1 typical clinical manifestations of psoriasis vulgaris.2 did not use glucocorticoid external drugs in nearly 2 weeks, and no systematic treatment had been accepted in nearly January. Treatment (especially with corticosteroids or immunosuppressants).3 except for autoimmune diseases (pemphigus, multiple dermatomyositis, systemic lupus erythematosus, rheumatoid arthritis) and other serious chronic systemic diseases, including 18 male, 11 female, age 19~52, average age of 31.60 + 9.29 years, and according to the condition of the disease. The case group was divided into two groups: 21 cases in the progressive group and 8 cases in the stationary phase group. The same staff were evaluated on the basis of PASI. 11 cases of health care workers in our hospital were healthy control group, including 5 males and 6 women. The average age was 25~40 years, the average age was (29.24 + 12.11) years, and there was no significant difference between sex, age and case group by t test. In the intravenous blood 2ml (including 10%EDTA1 mg/ml, aprotinin 500 kIU/ml) in the anticoagulant tube, centrifuge 3000rpm and 20min in the centrifuge, the collected serum specimens were stored in the refrigerator of -70 C. The expression level of IL-17? IL-22 in the serum samples was detected by the double antibody sandwich ELISA method, and the data were statistically analyzed with SPSS13.0 software. The data obtained by normality test are partial distribution, so the statistical method uses the non parametric test method of two independent sample rank conversion. The experimental results are described with median (four quantile spacing). The expression level of IL-17 and IL-22 in serum of psoriasis vulgaris, in the case group and the normal control group, the progressing group and the stationary phase group. There were statistically significant differences in the level of P0.05, and the relationship between the level of IL-17, IL-22 and the area of clinical lesions and severity (PASI score) in the serum of patients with psoriasis vulgaris was statistically analyzed by the two variable grade phase correlation analysis (Spearman method), and the test level was tested. P0.05 was statistically significant.
Results: the results of ELISA test showed that the expression of IL-17 in the serum of psoriasis vulgaris was higher than that of the normal control group (u=-1.696 P=0.045 P0.05), the difference was statistically significant, and the progression group was higher than the stationary phase group (u=-2.074 P=0.019 P0.05), the difference was statistically significant, the level of IL-17 expression in the blood and the clinical rash and severity (PASI evaluation). No linear correlation (r=-0.048 P=0.799 P0.05); the expression level of IL-22 in serum of patients with psoriasis vulgaris was higher than that of normal control group (u=-1.727 P=0.042 P0.05), the difference was statistically significant; the static period group was higher than the progression group (u=-0.049 P=0.480 P0.05), but the difference was not statistically significant, the expression level of IL-22 in the serum and clinical There was no linear correlation between rash and severity (PASI score) (r=-0.149 P=0.442 P0.05).
Conclusion:
The expression of IL-17 and IL-22 in serum of 1 psoriasis vulgaris patients is higher than that in the normal control group, which confirms that the expression of IL-17, IL-22 is closely related to the pathogenesis of psoriasis vulgaris, and indirectly reflects the important role of Th17 cells in the pathogenesis of psoriasis vulgaris.
The expression level of IL-17 in serum of 2 psoriasis vulgaris is higher than that in the stationary phase group, indicating that IL-17 plays a major role in the progression of psoriatic rashes, and may be related to the important function of the proinflammatory mediators as the main inflammatory mediators in the progression of inflammation.
The expression level of IL-17 and IL-22 in serum of psoriasis vulgaris has no correlation with the rash area of psoriasis and the PASI score of severity, indicating that the expression level of IL-17, IL-22 has no definite association with the area and severity of psoriasis skin lesions and does not reflect the severity of the disease.
【學(xué)位授予單位】:河北醫(yī)科大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2011
【分類號】:R758.63
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