【摘要】：白癜風(fēng)是一種常見(jiàn)多發(fā)的色素脫失性皮膚病,我國(guó)發(fā)病率為0.56%。盡管不直接造成身體傷害,白癜風(fēng)能嚴(yán)重影響患者的生活質(zhì)量。黑素細(xì)胞懸液移植是近年來(lái)治療白癜風(fēng)的熱門療法,針對(duì)治療過(guò)程中存在的活動(dòng)部位細(xì)胞移植難以固定和生長(zhǎng)環(huán)境的改變對(duì)移植細(xì)胞的損傷的難題,運(yùn)用組織工程技術(shù)構(gòu)建支持黑素細(xì)胞貼附、生長(zhǎng)和增殖的組織工程膜,能有效地解決上述問(wèn)題,從而改善治療效果。為此,本課題選用同軸電紡技術(shù),以醋酸為溶劑、PEO為核層、殼聚糖為殼層,制備核殼型殼聚糖納米纖維膜,詳細(xì)研究工藝條件對(duì)纖維成型及形貌的影響,并考察了戊二醛交聯(lián)對(duì)纖維膜性能及穩(wěn)定性的影響,用納米纖維膜培養(yǎng)黑素細(xì)胞,研究細(xì)胞的貼附、生長(zhǎng)和增殖情況。研究表明所制備的納米纖維膜存在與設(shè)計(jì)相一致的核殼型納米纖維;用戊二醛蒸汽對(duì)納米纖維膜進(jìn)行交聯(lián),提高了納米纖維膜的泡水質(zhì)量穩(wěn)定性和吸水率,同時(shí)提高膜的力學(xué)性能;該納米纖維膜能支持黑素細(xì)胞的貼附、生長(zhǎng)和增殖,其上的黑素細(xì)胞形態(tài)和功能表達(dá)正常,表明這種納米纖維膜可以作為黑素細(xì)胞組織工程膜。本工作的創(chuàng)新點(diǎn)有:利用同軸電紡技術(shù)制備了核殼型聚環(huán)氧乙烷-殼聚糖納米纖維膜,通過(guò)戊二醛交聯(lián)可提高纖維膜的力學(xué)性能和耐水穩(wěn)定性,纖維膜具有良好的生物相容性,能用作黑素細(xì)胞組織工程膜。
[Abstract]:Vitiligo is a common and frequent dermatosis with pigment loss. The incidence of vitiligo is 0.56 in China. Vitiligo can seriously affect the quality of life of patients, although it does not directly cause physical damage. Melanocyte suspension transplantation is a popular therapy for vitiligo in recent years. Tissue engineering technology is used to construct tissue engineering membrane supporting melanocyte adhesion, growth and proliferation, which can effectively solve the above problems and improve the therapeutic effect. In this paper, coaxial electrospinning technology was used to prepare core-shell chitosan nanofiber membrane with acetic acid as the solvent and chitosan as the shell layer. The effects of process conditions on the shaping and morphology of the fiber were studied in detail. The effects of glutaraldehyde crosslinking on the properties and stability of fiber membrane were investigated. Melanocytes were cultured with nano-fiber membrane to study the adhesion, growth and proliferation of the cells. The results show that there are core-shell nanofibers which are consistent with the design, and the crosslinking of nanofiber membranes by glutaraldehyde vapor can improve the water quality stability and water absorption of the nanofibers. The nano-fiber membrane can support the adhesion, growth and proliferation of melanocytes, and the morphology and function of melanocytes are normal, indicating that the nano-fiber membrane can be used as melanocyte tissue engineering membrane. The innovations of this work are as follows: Core-shell poly (ethylene oxide) chitosan nanofiber membranes were prepared by coaxial electrospinning. The mechanical properties and water resistance of the membranes were improved by glutaraldehyde crosslinking, and the fiber membranes had good biocompatibility. It can be used as melanocyte tissue engineering membrane.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R758.41;TQ340.64

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 孔玉龍;王克玉;張秀文;馬偉元;;羧甲基殼聚糖膜對(duì)人皮膚黑素細(xì)胞的生物相容性研究[J];中華皮膚科雜志;2014年11期

2 曹誼林;劉偉;張文杰;周廣東;;組織工程研究進(jìn)展[J];上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2012年09期

3 吳成;周曉鴻;;白癜風(fēng)細(xì)胞免疫研究新進(jìn)展[J];醫(yī)學(xué)綜述;2012年09期

4 孫良奎;程海峰;楚增勇;周永江;;同軸靜電紡絲再經(jīng)兩步后處理制備PAN基中空碳纖維[J];高分子學(xué)報(bào);2009年01期

5 張菊芳;沈海燕;王佳鳴;楊軍;;構(gòu)建含黑色素細(xì)胞組織工程表皮的實(shí)驗(yàn)研究[J];組織工程與重建外科雜志;2008年03期

6 Paul Bidez;Elizabeth Guterman-Tretter;Alan G. MacDiarmid;Peter I. Lelkes;危巖;;ELECTROACTIVE AND NANOSTRUCTURED POLYMERS AS SCAFFOLD MATERIALS FOR NEURONAL AND CARDIAC TISSUE ENGINEERING[J];Chinese Journal of Polymer Science;2007年04期

7 李大寧,劉進(jìn)芬,金文,張?jiān)＠?侯匯麗;白癜風(fēng)患者生活質(zhì)量的調(diào)查分析[J];臨床皮膚科雜志;2002年10期

8 朱惠光,計(jì)劍,高長(zhǎng)有,封麟先,沈家驄;聚乳酸組織工程支架材料[J];功能高分子學(xué)報(bào);2001年04期

相關(guān)碩士學(xué)位論文 前3條

1 蔣森陽(yáng);用于人體黑素細(xì)胞培養(yǎng)和移植的殼聚糖納米纖維膜的研究[D];浙江大學(xué);2015年

2 鄒潔輝;用于黑素細(xì)胞培養(yǎng)和移植的交聯(lián)殼聚糖膜材料的研究[D];浙江大學(xué);2013年

3 許思原;用于黑素細(xì)胞培養(yǎng)和轉(zhuǎn)移的殼聚糖及其復(fù)合膜材料的研究[D];浙江大學(xué);2011年

，

本文編號(hào)：2125940