缺氧誘導因子-1α、葡萄糖轉運蛋白-1、Ki-67在血管瘤和血管畸形中的表達及意義
發(fā)布時間：2018-07-14 20:06
【摘要】：目的檢測缺氧誘導因子-1α(hypoxia-inducible factor 1 alpha,HIF-1α)、葡萄糖轉運蛋白-1(glucose tracsporter-1,Glut-1)、Ki-67在不同時期血管瘤和血管畸形中的表達并探討其意義。 方法選取廣西醫(yī)科大學第一附屬醫(yī)院病理科2005年1月～2009年12月手術切除的血管瘤歸檔標本45例,常規(guī)石蠟切片蘇木精-伊紅染色法(hematoxylin-eosin staining,HE)進行病理復檢分組,另取10例正常兒童皮膚軟組織作對照;所有標本做厚度4μm連續(xù)切片,常規(guī)脫蠟及水化,采用免疫組織化學(Immunohistochemistry,IHC)鏈霉菌抗生物素蛋白-過氧化物酶連結法(streptavidin-perosidase,SP)處理,高溫高壓修復抗原,每張切片于高倍鏡下(400×)隨機選取具有代表性的5個視野,通過計數(shù)陽性細胞個數(shù)來觀察HIF-1α、Glut-1、Ki-67的表達。 結果45例血管瘤標本按Mulliken分類標準分為血管瘤30例,其中增生期19例,年齡3個月～1歲;消退期11例,年齡10個月～9歲;血管畸形15例,年齡7歲～52歲。HIF-1α陽性染色定位于血管內皮細胞的胞漿或胞核,呈棕黃色;增生期血管瘤內皮細胞的胞漿及胞核中有大量棕黃色顆粒,而消退期血管瘤、血管畸形及正常對照組只有少量棕黃色顆粒;統(tǒng)計結果表明增生期血管瘤中HIF-1α顯著高于消退期血管瘤、血管畸形及正常對照組,差異有統(tǒng)計學意義(P0.05)。Glut-1陽性染色定位于血管內皮細胞的胞膜或胞漿,呈棕黃色;增生期血管瘤內皮細胞的胞膜中有大量棕黃色顆粒,消退期血管瘤內皮細胞的胞膜有少量棕黃色顆粒,而血管畸形及正常對照組中內皮細胞的胞膜只有極少量或沒有棕黃色顆粒沉積;統(tǒng)計結果表明增生期血管瘤中Glut-1顯著高于消退期血管瘤,差異有統(tǒng)計學意義(P0.05),血管畸形及正常對照組中Glut-1全為陰性表達。Ki-67陽性染色定位于血管內皮細胞的胞核,呈棕黃色或棕褐色;增生期血管瘤內皮細胞的胞核中有大量棕黃色或棕褐色顆粒,而消退期血管瘤、血管畸形及正常對照組的內皮細胞的胞漿及胞核中只有少量棕黃色顆粒,統(tǒng)計結果表明增生期血管瘤中Ki-67顯著高于消退期血管瘤、血管畸形及正常對照組,差異有統(tǒng)計學意義(P0.05)。血管瘤中HIF-1α表達與Glut-1呈正相關(P0.05)。HIF-1α和Glut-1與Ki-67表達均呈正相關(P0.05)。 結論HIF-1α和Glut-1的異常表達與血管瘤的發(fā)生、發(fā)展有一定的關系,HIF-1α可能通過上調Glut-1的表達促進血管內皮細胞的增殖。
[Abstract]:Objective to detect the expression of hypoxia-inducible factor 1 alpha-HIF-1 偽 and glucose tracer 1 Glut-1 in different stages of hemangioma and vascular malformation. Methods from January 2005 to December 2009, 45 cases of hemangioma archived from Department of Pathology, first affiliated Hospital of Guangxi Medical University, were divided into two groups: routine paraffin section hematoxylin-eosin staining method (HE). The skin and soft tissue of 10 normal children were taken as control, all the specimens were made 4 渭 m thick serial sections, conventional dewaxing and hydration, and were treated with streptavidin-perosidase SP (streptavidin-perosidase SP) by Immunohistochemistry (IHC). The antigens were repaired at high temperature and high pressure. Five representative visual fields were randomly selected under high-power microscope (400 脳). The expression of HIF-1 偽 -Glut-1 Ki-67 was observed by counting the number of positive cells. Results according to Mulliken classification criteria, 45 hemangioma specimens were divided into 30 cases, including 19 cases in proliferative phase, 11 cases in regression phase, 10 months old and 9 years old, 15 cases in vascular malformation, 19 cases in proliferative phase, 1 year old in 3 months and 1 year old, 15 cases in vascular malformation. The positive staining of HIF-1 偽 was located in the cytoplasm or nucleus of vascular endothelial cells, and was brown in the cytoplasm and nucleus of proliferative hemangioma endothelial cells, while in the involuted phase, a large number of brown granules were found in the cytoplasm and nucleus of the endothelial cells of hemangioma. The statistical results showed that HIF-1 偽 in proliferative hemangioma was significantly higher than that in involutive hemangioma, vascular malformation and normal control group. The difference was statistically significant (P0.05). Glut-1 positive staining was located in the membrane or cytoplasm of vascular endothelial cells, and there were a lot of brown granules in the membranes of proliferative hemangioma endothelial cells. A small amount of brown granules were found in the membrane of endothelial cells in involutive phase, but only a few or no brown granules were found in the membrane of endothelial cells in vascular malformation and normal control group. The statistical results showed that Glut-1 in proliferative hemangioma was significantly higher than that in involutive hemangioma (P0.05). The expression of Glut-1 in vascular malformation and normal control group was all negative. Ki-67 positive staining was localized in the nucleus of vascular endothelial cells. A large number of brown or brown granules were found in the nucleus of endothelial cells in proliferative hemangioma, but only a few brown granules in the cytoplasm and nucleus of endothelial cells in involuted hemangioma, vascular malformation and normal control group. The statistical results showed that Ki-67 in proliferative hemangioma was significantly higher than that in involutive hemangioma, vascular malformation and normal control group (P0.05). HIF-1 偽 expression was positively correlated with Glut-1 expression (P0.05). HIF-1 偽 and Glut-1 expression were positively correlated with Ki-67 expression (P0.05). Conclusion the abnormal expression of HIF-1 偽 and Glut-1 is related to the occurrence and development of hemangioma. HIF-1 偽 may promote the proliferation of vascular endothelial cells by up-regulating the expression of Glut-1.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R739.5
本文編號：2122803
[Abstract]:Objective to detect the expression of hypoxia-inducible factor 1 alpha-HIF-1 偽 and glucose tracer 1 Glut-1 in different stages of hemangioma and vascular malformation. Methods from January 2005 to December 2009, 45 cases of hemangioma archived from Department of Pathology, first affiliated Hospital of Guangxi Medical University, were divided into two groups: routine paraffin section hematoxylin-eosin staining method (HE). The skin and soft tissue of 10 normal children were taken as control, all the specimens were made 4 渭 m thick serial sections, conventional dewaxing and hydration, and were treated with streptavidin-perosidase SP (streptavidin-perosidase SP) by Immunohistochemistry (IHC). The antigens were repaired at high temperature and high pressure. Five representative visual fields were randomly selected under high-power microscope (400 脳). The expression of HIF-1 偽 -Glut-1 Ki-67 was observed by counting the number of positive cells. Results according to Mulliken classification criteria, 45 hemangioma specimens were divided into 30 cases, including 19 cases in proliferative phase, 11 cases in regression phase, 10 months old and 9 years old, 15 cases in vascular malformation, 19 cases in proliferative phase, 1 year old in 3 months and 1 year old, 15 cases in vascular malformation. The positive staining of HIF-1 偽 was located in the cytoplasm or nucleus of vascular endothelial cells, and was brown in the cytoplasm and nucleus of proliferative hemangioma endothelial cells, while in the involuted phase, a large number of brown granules were found in the cytoplasm and nucleus of the endothelial cells of hemangioma. The statistical results showed that HIF-1 偽 in proliferative hemangioma was significantly higher than that in involutive hemangioma, vascular malformation and normal control group. The difference was statistically significant (P0.05). Glut-1 positive staining was located in the membrane or cytoplasm of vascular endothelial cells, and there were a lot of brown granules in the membranes of proliferative hemangioma endothelial cells. A small amount of brown granules were found in the membrane of endothelial cells in involutive phase, but only a few or no brown granules were found in the membrane of endothelial cells in vascular malformation and normal control group. The statistical results showed that Glut-1 in proliferative hemangioma was significantly higher than that in involutive hemangioma (P0.05). The expression of Glut-1 in vascular malformation and normal control group was all negative. Ki-67 positive staining was localized in the nucleus of vascular endothelial cells. A large number of brown or brown granules were found in the nucleus of endothelial cells in proliferative hemangioma, but only a few brown granules in the cytoplasm and nucleus of endothelial cells in involuted hemangioma, vascular malformation and normal control group. The statistical results showed that Ki-67 in proliferative hemangioma was significantly higher than that in involutive hemangioma, vascular malformation and normal control group (P0.05). HIF-1 偽 expression was positively correlated with Glut-1 expression (P0.05). HIF-1 偽 and Glut-1 expression were positively correlated with Ki-67 expression (P0.05). Conclusion the abnormal expression of HIF-1 偽 and Glut-1 is related to the occurrence and development of hemangioma. HIF-1 偽 may promote the proliferation of vascular endothelial cells by up-regulating the expression of Glut-1.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R739.5
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，本文編號：2122803
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