發(fā)布時(shí)間：2018-07-02 15:12

  本文選題：復(fù)方茯苓湯 + 梔子苷��； 參考：《大連醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的:濕疹是常見的炎癥性皮膚病,瘙癢劇烈、反復(fù)發(fā)作,對(duì)患者生活影響嚴(yán)重。其發(fā)生與Ⅳ型遲發(fā)型變態(tài)反應(yīng)有一定關(guān)系,T輔助細(xì)胞( Th1/ Th2)的失衡在濕疹的發(fā)病中起到了重要的作用。前期實(shí)驗(yàn)研究和臨床應(yīng)用證明復(fù)方茯苓湯治療濕疹有明顯療效,無明顯毒副作用。復(fù)方茯苓湯是由茯苓、赤芍、梔子、澤瀉、黃柏、當(dāng)歸、浮萍、甘草八味中藥組成的復(fù)方湯劑,梔子苷是復(fù)方茯苓湯的主要活性成分之一,京尼平為梔子苷體內(nèi)的代謝產(chǎn)物,是梔子苷主要入血成分,兩者具有抗炎、解熱、利膽和輕瀉等多種生物學(xué)活性,但對(duì)T細(xì)胞作用的影響尚不清楚。在炎癥反應(yīng)中,T細(xì)胞對(duì)免疫識(shí)別、免疫應(yīng)答和免疫調(diào)控起著關(guān)鍵的作用。本課題以人外周血T淋巴細(xì)胞為模型,探討復(fù)方茯苓湯中有效化學(xué)成分梔子苷和京尼平對(duì)T細(xì)胞增殖和產(chǎn)生Th1或Th2型細(xì)胞因子作用的影響,為治療濕疹及進(jìn)一步研究開發(fā)為治療新藥提供實(shí)驗(yàn)依據(jù)。 方法:采用SRB法檢測(cè)PMA活化的人外周血T淋巴細(xì)胞的增殖及梔子苷及京尼平對(duì)其增殖的抑制,采用ELISA法分析梔子苷和京尼平對(duì)活化的人外周血T淋巴細(xì)胞上清中IFN-γ和IL-4的影響。 結(jié)果:不同濃度梔子苷及京尼平均可以抑制PMA活化的人外周血T淋巴細(xì)胞的增殖(P0.01),且呈濃度依賴性。不同濃度的梔子苷及京尼平可顯著降低活化人外周血T淋巴細(xì)胞上清液中IFN-γ的水平(P0.01),而對(duì)活化人外周血T淋巴細(xì)胞上清液中IL-4水平的無顯著的影響(P0.01)。 結(jié)論:復(fù)方茯苓湯中梔子苷及其代謝產(chǎn)物京尼平可抑制PMA誘導(dǎo)的人外周血T淋巴細(xì)胞的增殖,梔子苷及京尼平可顯著降低活化人外周血T淋巴細(xì)胞上清液中IFN-γ的水平(P0.01),其抗炎機(jī)制可能是通過抑制T淋巴細(xì)胞的增殖,降低IFN-γ的水平來實(shí)現(xiàn)的。
[Abstract]:Objective: eczema is a common inflammatory skin disease. The pathogenesis of Th _ 1 / Th2 may play an important role in the pathogenesis of eczema. Experimental study and clinical application showed that compound Fuling decoction had obvious curative effect on eczema without obvious side effects. Compound Fuling decoction is a compound decoction composed of Poria cocos, Radix Paeoniae Alba, Gardenia Gardenia, Fructus Alismatis, Cortex Phellodendri, Angelica sinensis, duckweed, Glycyrrhiza and Radix Glycyrrhizae. Geniposide is one of the main active components of compound Fuling decoction, and genipin is the metabolite of Gardenia glycoside. Gardenia glycoside is the main component of blood, both of them have many biological activities, such as anti-inflammatory, antipyretic, choledochal and catharsis, but the effect on T cells is not clear. In inflammatory response, T cells play a key role in immune recognition, immune response and immune regulation. Using human peripheral blood T lymphocytes as a model, the effects of geniposide and geniapine on the proliferation of T cells and the production of Th1 or Th2 cytokines in compound Fuling decoction were studied. For the treatment of eczema and further research and development for the treatment of new drugs to provide experimental basis. Methods: SRB method was used to detect the proliferation of human peripheral blood T lymphocytes activated by PMA and the inhibition of geniposide and geniposide on the proliferation. The effects of geniposide and geniposide on IFN- 緯 and IL-4 in the supernatant of activated human peripheral T lymphocytes were analyzed by Elisa. Results: geniposide and geniposide at different concentrations could inhibit the proliferation of human peripheral blood T lymphocytes activated by PMA (P0.01) in a dose-dependent manner. Different concentrations of geniposide and geniposide significantly decreased the level of IFN- 緯 in the supernatant of activated human peripheral blood T lymphocytes (P0.01), but had no significant effect on the level of IL-4 in the supernatant of activated human peripheral T lymphocytes (P0.01). Conclusion: Geniposide and its metabolite geniposide in compound Fuling decoction can inhibit the proliferation of human peripheral blood T lymphocytes induced by PMA. Geniposide and geniposide significantly decreased the level of IFN- 緯 in the supernatant of activated human peripheral T lymphocytes (P0.01). The anti-inflammatory mechanism may be achieved by inhibiting the proliferation of T lymphocytes and decreasing the level of IFN- 緯.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R758.23;R285.5

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