本文選題：瘢痕癌 + 細(xì)胞周期調(diào)控　； 參考：《醫(yī)學(xué)研究生學(xué)報》2014年09期

【摘要】：目的細(xì)胞周期調(diào)控機制失調(diào)是細(xì)胞增生腫瘤發(fā)生的重要因素。文中探討細(xì)胞周期調(diào)控系統(tǒng)相關(guān)因子Cyclin D1-CDK4-p21在瘢痕癌中的表達(dá)及意義。方法選取遵義醫(yī)學(xué)院病理教研室和中山大學(xué)附屬第五醫(yī)院病理科2005-2011年石蠟包埋標(biāo)本,分為瘢痕癌組、病理性瘢痕組和正常皮膚組。應(yīng)用組織化學(xué)法,分別檢測瘢痕癌、病理性瘢痕和正常皮膚組織中Cyclin D1、CDK4、p21蛋白的表達(dá)。采用核酸分子原位雜交技術(shù)檢測Cyclin D1 mRNA、CDK4 mRNA、p21 mRNA在3組組織中的表達(dá)。對各項指標(biāo)進(jìn)行相關(guān)性分析,計算平均光密度(表達(dá)強度)和陽性面積(表達(dá)水平)。結(jié)果 1Cyclin D1、CDK4、p21蛋白和Cyclin D1 mRNA、CDK4 mRNA、p21 mRNA在瘢痕癌癌組織呈強陽性表達(dá),在病理性瘢痕上皮中呈弱陽性表達(dá),在正常皮膚表皮呈弱陽性或陰性表達(dá)。瘢痕癌組CDK4蛋白表達(dá)強度(0.273±0.024)及表達(dá)水平(0.159±0.036)較正常皮膚組(0.194±0.013、0.053±0.086)、病理性瘢痕組(0.214±0.026、0.061±0.014)升高,瘢痕癌組CDK4 mRNA表達(dá)強度(0.281±0.033)、表達(dá)水平(0.207±0.039)較正常皮膚組(0.195±0.012、0.067±0.027)、病理性瘢痕組(0.235±0.021、0.080±0.032)高,差異有統(tǒng)計學(xué)意義(P0.01);瘢痕癌組Cyclin D1蛋白表達(dá)強度(0.262±0.023)、表達(dá)水平(0.141±0.036)較正常皮膚組(0.169±0.012、0.039±0.095)及病理性瘢痕組(0.176±0.039、0.065±0.013)高,瘢痕癌組Cyclin D1mRNA表達(dá)強度(0.264±0.031)、表達(dá)水平(0.201±0.041)較正常皮膚組(0.179±0.022、0.049±0.083)及病理性瘢痕組(0.193±0.021、0.068±0.035)高,差異均有統(tǒng)計學(xué)意義(P0.01);瘢痕癌組p21蛋白表達(dá)強度(0.148±0.031)、表達(dá)水平(0.275±0.032)較正常皮膚組(0.052±0.020、0.197±0.036)及病理性瘢痕組(0.062±0.021、0.214±0.032)高;瘢痕癌組p21 mRNA表達(dá)強度(0.227±0.059)較正常皮膚組(0.072±0.044、0.203±0.024)、病理性瘢痕組(0.075±0.041、0.223±0.021)高,差異均有統(tǒng)計學(xué)意義(P0.01);但病理性瘢痕組與正常皮膚組各項指標(biāo)比較,差異無統(tǒng)計學(xué)意義(P0.05)。2相關(guān)性分析:在瘢痕癌組織中,Cyclin D1與CDK4、p21與CDK4的表達(dá)均呈正相關(guān)。結(jié)論瘢痕癌的發(fā)生與Cyclin D1、CDK4的異常表達(dá)有關(guān),Cyclin D1可能是通過與CDK4結(jié)合形成復(fù)合物,促進(jìn)細(xì)胞周期G1/S期轉(zhuǎn)化,導(dǎo)致細(xì)胞異常增生,瘢痕癌發(fā)生。瘢痕癌中Cyclin D1-CDK4復(fù)合物活性的抑制調(diào)控,可能是CKI家族的其他成員或者有CKI家族以外的其他抑制調(diào)控因子。
[Abstract]:Objective the dysregulation of cell cycle is an important factor in tumorigenesis of cell proliferation. To investigate the expression and significance of cell cycle regulation system related factor Cyclin D1-CDK4-p21 in scar carcinoma. Methods paraffin embedded specimens were collected from Department of Pathology, Zunyi Medical College and Department of Pathology of the Fifth affiliated Hospital of Sun Yat-sen University from 2005 to 2011. The specimens were divided into three groups: scar carcinoma group, pathological scar group and normal skin group. The expression of Cyclin D1-CDK4p21 protein in scar carcinoma, pathological scar and normal skin was detected by histochemical method. In situ hybridization was used to detect the expression of Cyclin D1 mRNA-CDK4 mRNAp21 mRNA in three groups of tissues. The average optical density (expression intensity) and positive area (expression level) were calculated by correlation analysis. Results 1Cyclin D1 mRNA-CDK4 mRNAp21 protein and Cyclin D1 mRNA-CDK4 mRNAp21 mRNA were strongly expressed in scar carcinoma, weakly positive in pathological scar epithelium and weakly positive or negative in normal skin epidermis. The expression of CDK4 protein in scar cancer group was 0.273 鹵0.024) and the expression level was 0.159 鹵0.036), which was higher than that in normal skin group (0.194 鹵0.013) 0.053 鹵0.086 and pathological scar group (0.214 鹵0.026) 0.061 鹵0.014). The expression level of CDK4 mRNA in scar carcinoma group was 0.281 鹵0.033 and 0.207 鹵0.039) higher than that in normal skin group (0.195 鹵0.012) and pathological scar group (0.235 鹵0.021) 0.080 鹵0.032). The expression intensity of Cyclin D1 protein in scar cancer group was 0.262 鹵0.023 (0.141 鹵0.036) higher than that in normal skin group (0.169 鹵0.012 鹵0.039 鹵0.095) and pathological scar group (0.176 鹵0.039 鹵0.065 鹵0.013). The expression intensity of Cyclin D1mRNA in scar carcinoma group was 0.264 鹵0.031 and 0.201 鹵0.041) higher than that in normal skin group (0.179 鹵0.022) and pathological scar group (0.193 鹵0.021 鹵0.068 鹵0.035). The expression intensity of p21 protein in scar carcinoma group was higher than that in normal skin group (0. 052 鹵0. 020) and pathological scar group (0. 062 鹵0. 021 ~ 0. 214 鹵0. 032), and the expression intensity of p21 mRNA in scar carcinoma group was 0. 227 鹵0. 059) higher than that in normal skin group (0. 072 鹵0. 0440.203 鹵0. 024) and pathological scar group (0. 075 鹵0. 041 鹵0. 0223 鹵0. 021). The difference was statistically significant (P 0.01), but there was no significant difference between the pathological scar group and the normal skin group. The correlation analysis showed that the expression of Cyclin D1, CDK4p21 and CDK4 were positively correlated in the scar carcinoma tissues. Conclusion the occurrence of scar carcinoma is related to the abnormal expression of Cyclin D1 + CDK4, which may be related to the formation of complex with CDK4 and promote the transformation of G 1 / S phase of cell cycle, leading to abnormal proliferation of cells and carcinogenesis of scar. The inhibitory regulation of Cyclin D1-CDK4 complex activity in scar carcinoma may be other members of the CKI family or other inhibitory regulators other than the CKI family.
【作者單位】： 遵義醫(yī)學(xué)院珠海校區(qū)病理學(xué)教研室;中山大學(xué)附屬第五醫(yī)院病理科;
【基金】：貴州省科技攻關(guān)項目(2010-3080)
【分類號】：R739.5

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