本文選題：馬拉色菌 + 鑒定　； 參考：《復(fù)旦大學(xué)》2010年碩士論文

【摘要】： 第一部分：馬拉色菌屬的分類及其在常見皮膚病的分布情況 目的：研究馬拉色菌屬的分類及其在兩種常見馬拉色菌相關(guān)疾病中的分布情況。 方法：以標(biāo)準(zhǔn)株作為對照,采用PCR擴增rDNA ITS2區(qū)并測序的分子生物學(xué)方法將來源于花斑糠疹和馬拉色菌毛囊炎的馬拉色菌進(jìn)行分類和描述,分析各菌種在這兩種疾病的分布情況。 結(jié)果：在135例花斑糠疹和馬拉色菌毛囊炎分離到6個菌種,共114株馬拉色菌。鑒定出球形馬拉色菌47株(41%)、合軸馬拉色菌40株(35%)、糠秕馬拉色菌20株(17.5%)、鈍形馬拉色菌4株(3.5%)、大和馬拉色菌2株(1.8%)、皮炎馬拉色菌1株(0.9%)。兩種疾病菌種構(gòu)成與疾病類型有關(guān),近半數(shù)花斑癬患者皮損中分離出合軸馬拉色菌,大部分馬拉色菌毛囊炎中分離出球形馬拉色菌。 結(jié)論：不同疾病馬拉色菌菌種構(gòu)成不同,球形馬拉色菌與馬拉色菌毛囊炎密切相關(guān)；馬拉色菌rDNA的ITS2區(qū)PCR擴增與測序可用于馬拉色菌的分類鑒定。 第二部分：利用Biolog微生物自動分析系統(tǒng)建立常見馬拉色菌的鑒定數(shù)據(jù)庫 目的：利用Biolog微生物自動分析系統(tǒng)建立常見馬拉色菌的鑒定數(shù)據(jù)庫,探討該系統(tǒng)鑒定馬拉色菌的應(yīng)用前景。 方法：采用表型及PCR擴增、測序的方法,將臨床收集的菌株鑒定至種；選取臨床最常見的糠秕、合軸和球形馬拉色菌,接種于FF微孔板,記錄常見馬拉色菌對95種不同碳源的利用情況,描述其各自的生長反應(yīng)譜,建立鑒定數(shù)據(jù)庫。 結(jié)果：三種馬拉色菌中絕大部分菌株可以利用吐溫80、糊精；所有馬拉色菌菌株均不能利用D-環(huán)式糊精、D-半乳糖醛酸、癸二酸；三種馬拉色菌對碳源的利用具有明顯的差別,而同種馬拉色菌對碳源的利用趨勢基本相同�？凤躐R拉色菌可以利用N-乙酰-D-葡萄糖胺、D-纖維二糖、i-赤藻糖醇、D-果糖等；而球形和合軸馬拉色菌均可以利用D-阿拉伯糖、L-阿拉伯糖、D-核糖和D-木糖；其中合軸馬拉色菌可以利用甘油／丙三醇。 結(jié)論：Biolog微生物鑒定系統(tǒng)采用一種特殊的表型鑒定方法,可以對該三種常見馬拉色菌進(jìn)行鑒定,初步推測該系統(tǒng)在馬拉色菌不同菌種鑒定中的應(yīng)用前景。
[Abstract]:Part I: classification of Malassezia and its distribution in common skin diseases Objective: to study the classification of Malassezia and its distribution in two common malassezia related diseases. Methods: Malassezia derived from pityriasis florescens and Malassezia folliculitis were classified and described by PCR amplification of rDNA ITS2 region and sequencing. The distribution of each strain in the two diseases was analyzed. Results: totally 114 strains of Malassezia were isolated from 135 cases of furfurous plaque and Malassezia folliculitis. A total of 47 strains of Malassezia globosa were identified, 40 strains of Malassezia spp., 20 strains of Malassezia furfura, 4 strains of Malassezia obtuse Malassezia, 2 strains of Malassezia grandis, 1 strain of Malassezia dermatitis, and 1 strain of Malassezia dermatitis were identified. The composition of the two diseases was related to the type of disease. Malassezia sphaeroides was isolated from the skin lesions of nearly half of the patients with tinea versicolor and Malassezia sphaeroides was isolated from most of the malassezia folliculitis. Conclusion: Malassezia sphaeroides is closely related to Malassezia folliculitis and the ITS2 region PCR amplification and sequencing of Malassezia rDNA can be used for classification and identification of Malassezia. Part two: the establishment of identification database of common Malassezia by Biolog microorganism automatic analysis system Objective: to establish a database for the identification of common Malassezia by Biolog microbiological automatic analysis system, and to explore the application prospect of the system in the identification of Malassezia. Methods: phenotypic, PCR amplification and sequencing methods were used to identify the strains collected in clinic, and the most common clinical strains, chaff, rachis and Malassezia globularis, were inoculated on FF microporous plate. The utilization of 95 different carbon sources of Malassezia was recorded, their growth response spectra were described, and the identification database was established. Results: most of the three strains of Malassezia could use Tween 80 and dextrin, all Malassezia strains could not use D-cyclodextrin D-galacturonic acid and sebacic acid. The trend of carbon utilization of Malassezia homogenate was basically the same. Malassezia furfuris can use N- acetyl-D- glucosamine D- fiberdisaccharide-alginate, D- fructose, etc., while D- arabinose L- arabinose D- ribose and D- xylose can be used by Malassezia sphaeraceus, and D- arabinose L- arabinose D- ribose and D- xylose. Malassezia can use glycerol / glycerol. Conclusion A special phenotypic identification method was used to identify the three common Malassezia species in the microbiological identification system. The application prospect of the system in different species identification of Malassezia was preliminarily speculated.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R756

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