發(fā)布時(shí)間：2018-05-13 14:19

  本文選題：尖銳濕疣 + 流式細(xì)胞術(shù)　； 參考：《山西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：測定尖銳濕疣患者外周血趨化因子受體3(CXCR3)和趨化因子受體4(CCR4)在外周血T淋巴細(xì)胞的表達(dá)，通過其在CD4+T細(xì)胞及CD8+T細(xì)胞比值的變化，，探討趨化因子及其受體在尖銳濕疣發(fā)病中的作用。方法：采用免疫熒光標(biāo)記—流式細(xì)胞儀檢測尖銳濕疣患者和正常人外周血CD4+T細(xì)胞及CD8+T細(xì)胞的CXCR3和CCR4表達(dá)水平，并計(jì)算CXCR3和CCR4比值的變化。 結(jié)果：尖銳濕疣患者外周血CD4+T淋巴細(xì)胞CXCR3的表達(dá)與正常對(duì)照組比較無顯著性差異(P0.05)，而CCR4的表達(dá)顯著增多，具有統(tǒng)計(jì)學(xué)意義(P0.05)；尖銳濕疣患者組CD4+CXCR3/CD4+CCR4比值顯著降低(P0.05)；尖銳濕疣患者外周血CD8+T淋巴細(xì)胞CXCR3的表達(dá)水平明顯高于正常對(duì)照組（P0.01）；尖銳濕疣患者外周血CD8+T淋巴細(xì)胞CCR4較低，且與正常對(duì)照組比較無統(tǒng)計(jì)學(xué)意義(P0.05)，其比值有統(tǒng)計(jì)學(xué)差異（P0.01）；CD4+T淋巴細(xì)胞和CD8+T淋巴細(xì)胞的CXCR3與CCR4的表達(dá)水平均無相關(guān)性（P0.05)。 結(jié)論：尖銳濕疣患者外周血CD4+T淋巴細(xì)胞的CCR4呈高表達(dá)，CD8+T淋巴細(xì)胞CXCR3表達(dá)水平高于正常對(duì)照組，表明機(jī)體在感染HPV后，細(xì)胞免疫狀態(tài)發(fā)生改變，出現(xiàn)尖銳濕疣的患者外周血Th2和CTL細(xì)胞增多，一方面可能存在Th1向Th2漂移現(xiàn)象，Th1/Th2失衡，產(chǎn)生免疫逃逸，另一方面，CTL細(xì)胞增多有利于機(jī)體更好的清除HPV病毒。
[Abstract]:Objective: to determine the expression of chemokine receptor (CXCR3) and chemokine receptor (CCR4) in peripheral blood T lymphocytes of patients with condyloma acuminatum, and to determine the ratio of CXCR3 and CCR4 in CD4 T cells and CD8 T cells. To investigate the role of chemokines and their receptors in the pathogenesis of condyloma acuminatum. Methods: the expressions of CXCR3 and CCR4 in CD4 T cells and CD8 T cells in peripheral blood of patients with condyloma acuminatum and normal subjects were detected by immunofluorescence labeling and flow cytometry, and the ratio of CXCR3 and CCR4 was calculated. Results: there was no significant difference in CXCR3 expression of CD4 T lymphocytes between patients with condyloma acuminatum and normal control group (P 0.05), but the expression of CCR4 was significantly increased in patients with condyloma acuminatum. The ratio of CD4 CXCR3/CD4 CCR4 in patients with condyloma acuminatum was significantly lower than that in patients with condyloma acuminatum, the expression of CXCR3 in peripheral blood CD8 T lymphocytes in patients with condyloma acuminatum was significantly higher than that in normal control group, the CCR4 of CD8 T lymphocytes in patients with condyloma acuminatum was lower than that in patients with condyloma acuminatum. There was no significant difference in the ratio of CXCR3 and CCR4 between P0.01 T lymphocytes and CD8 T lymphocytes compared with the normal control group (P 0.05). Conclusion: the CCR4 expression of CD4 T lymphocytes in patients with condyloma acuminatum is higher than that of normal controls, which indicates that the cellular immune state changes after infection of HPV in patients with condyloma acuminatum. In patients with condyloma acuminatum, the number of Th2 and CTL cells in peripheral blood increased. On the one hand, there may be the imbalance of Th1 / Th2 shift from Th1 to Th2 and immune escape. On the other hand, the increase of CTL cells is beneficial to the elimination of HPV virus.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R752.53

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳興平,張杏平;TAP1及其mRNA在尖銳濕疣皮損中的表達(dá)[J];中國麻風(fēng)皮膚病雜志;2005年03期

2 鄭紅;趨化因子及其受體的功能[J];免疫學(xué)雜志;2004年01期

3 王玉坤,張利寧,王克玉,梁臨平;尖銳濕疣患者HPV型與細(xì)胞免疫功能及復(fù)發(fā)關(guān)系的研究[J];山東醫(yī)科大學(xué)學(xué)報(bào);2001年03期

4 ;尖銳濕疣患者血清IL-2及sIL-2R水平的觀察[J];上海免疫學(xué)雜志;1999年02期

5 呂蓉;尖銳濕疣患者免疫功能的研究進(jìn)展[J];中國艾滋病性病;2003年03期

6 劉冬先;江文;陳興平;陳映玲;楊東亮;;尖銳濕疣患者外周血CD4~+ T細(xì)胞細(xì)胞因子的檢測[J];中國皮膚性病學(xué)雜志;2007年07期

7 李文維,曹索奇;性激素與尖銳濕疣人乳頭瘤病毒的相關(guān)性研究[J];中國皮膚性病學(xué)雜志;1998年02期

8 李其林,沈雁;尖銳濕疣患者外周血T淋巴細(xì)胞亞群及胸腺肽治療的研究[J];中國皮膚性病學(xué)雜志;1999年02期

9 錢起豐,莫衍石;尖銳濕疣患者白介素12、白介素15和腫瘤壞死因子α水平的研究[J];中華皮膚科雜志;1997年03期

10 王嵐,陳映玲,周禮義;尖銳濕疣患者腫瘤壞死因子α、白介素6和白介素8水平的研究[J];中華皮膚科雜志;1998年04期

本文編號(hào)：1883553