本文選題：銀屑病 + 人DMSCs細胞　； 參考：《山西醫(yī)科大學》2015年碩士論文

【摘要】：目的:利用Transwell小室技術共同培養(yǎng)人真皮間充質干細胞(Human dermis mesenchymal stem cells,DMSCs)與Ha-cat細胞(人永生化角質形成細胞株);探明人DMSCs對Ha-cat細胞增殖的作用。方法:1、機械-酶順序分離法分離銀屑病患者和正常人的真皮與表皮,培養(yǎng)、純化DMSCs;2、采用FCM檢測Ha-cat細胞表面標記物;3、多向分化法誘導人DMSCs細胞分化,鑒定細胞的生物學特性;4、采用RTCA(實時動態(tài)細胞分析系統(tǒng))檢測Ha-cat細胞的增殖曲線;5、檢測DMSCs誘導后的Ha-cat細胞的活細胞數(shù)量;6、用FCM檢測Ha-cat細胞的增殖周期。結果:1、銀屑病患者組與正常人組DMSCs細胞在形態(tài)與培養(yǎng)方法上無顯著的差異;2、FCM檢測人DMSCs表面標記物:CD29、CD44、CD73、CD90、CD105及CD105均呈高表達,而CD34、CD45及HLA-DR表達陰性,表明DMSCs有較高的純度;3、成功使用化學試劑誘導DMSCs定向分化,使之分化為脂肪等多種組織細胞;4、x CELLigence檢測結果表明:銀屑病患者組較正常人對照組DMSCs細胞促進Ha-cat細胞的增殖;(患者組Ha-cat細胞CIMax值為5.55,對照組Ha-cat細胞CIMax值為5.40)5、細胞計數(shù)儀檢測:銀屑病患者組Ha-cat活細胞數(shù)較正常對照組有所增加;6、FCM檢測細胞的增殖周期:銀屑病組Ha-cat細胞進入S期的細胞比例增加。(p0.05)。結論:1、成功模擬生物狀態(tài)下人DMSCs與Ha-cat細胞(人永生化角質形成細胞株)的相互作用;2、銀屑病表皮的異常增厚與人DMSCs相關。
[Abstract]:Aim: to investigate the effect of human DMSCs on the proliferation of human dermal mesenchymal stem cells (DMSCs) and Ha-cat cells (human immortalized keratinocytes) by co-culture of human dermal mesenchymal stem cells (dermis mesenchymal stem cells) with Transwell chamber technique. Methods the dermis and epidermis of psoriasis patients and normal persons were separated by mechanical-enzymatic sequential separation method. DMSCs2 was cultured and purified. The surface marker of Ha-cat cells was detected by FCM. The differentiation of human DMSCs cells was induced by multiple differentiation method. The biological characteristics of the cells were identified. The proliferation curve of Ha-cat cells was detected by RTCA (Real time dynamic Cell Analysis system), the number of living cells induced by DMSCs was detected and the proliferative cycle of Ha-cat cells was detected by FCM. Results there was no significant difference in morphology and culture methods of DMSCs cells between psoriatic patients and normal controls. FCM was used to detect the high expression of CD105 and CD105 in human DMSCs surface markers: 1. The expression of CD34 + CD45 and HLA-DR was negative. The results showed that DMSCs had a high purity and was successfully induced by chemical reagents to induce the directional differentiation of DMSCs. The results of CELLigence analysis showed that DMSCs cells in psoriatic patients promoted the proliferation of Ha-cat cells (CIMax value of Ha-cat cells was 5.55, CIMax value of Ha-cat cells in control group was 5.40%, and that of control group was 5.40%). The number of Ha-cat living cells in psoriatic patients was increased compared with the control group. The proliferative cycle of Ha-cat cells in psoriasis group was increased. The percentage of Ha-cat cells entering S phase was increased in psoriasis group. Conclusion the interaction between human DMSCs and Ha-cat cells (human immortalized keratinocyte line) was successfully simulated under biological condition. The abnormal thickening of psoriatic epidermis was related to human DMSCs.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R758.63

【參考文獻】

相關期刊論文 前4條

1 徐成陽;馮德廣;杜英;趙根尚;蔣素娟;余海濱;于海明;黃玉敏;;人骨髓間充質干細胞誘導分化為內皮細胞的實驗研究[J];中原醫(yī)刊;2006年08期

2 郭新;李玉林;何旭;李偉;李一雷;張麗紅;楊瑞光;趙維;;人骨髓間充質干細胞體外內皮細胞誘導分化的研究(英文)[J];中國病理生理雜志;2006年08期

3 石繼海,夏隆慶,王群,王大光,鞠強,辛燕,康曉靜,何秀珍,隋連金,鐘宇眉,邵長庚;不同嚴重程度銀屑病對患者生活質量的影響[J];中華皮膚科雜志;2003年03期

4 晏開力;汪健;李慶;鄔志偉;徐修才;徐靜瑋;翟志敏;;成人骨髓間充質干細胞分化為血管內皮細胞的研究[J];中國修復重建外科雜志;2007年01期

，

本文編號：1840799