本文選題：輔酶Q10 + 納米結(jié)構(gòu)脂質(zhì)載體��； 參考：《清華大學(xué)》2010年碩士論文

【摘要】：皮膚光老化是皮膚衰老的重要原因之一,日光紫外線主要是通過誘導(dǎo)皮膚細(xì)胞產(chǎn)生活性氧自由基和氧化壓力來損傷細(xì)胞和皮膚。輔酶Q10(CoQ10)是一種極具潛力的抗氧劑,然而由于其自身的難溶性特性限制了它在抗紫外線引起的皮膚光老化方面的應(yīng)用。納米結(jié)構(gòu)脂質(zhì)載體系統(tǒng)(NLC)是一種新型的給藥系統(tǒng),具有增加藥物溶解能力、提高制劑穩(wěn)定性、增強(qiáng)皮膚滲透能力和降低刺激性等優(yōu)點。本課題的主要目的是設(shè)計并制備一種新型的經(jīng)皮納米給藥系統(tǒng)來運(yùn)載CoQ10,并對其各類功能性質(zhì)進(jìn)行研究和評價,期望能夠得到一種高效、靶向、安全的經(jīng)皮給藥系統(tǒng)。 為改善CoQ10在保護(hù)皮膚免受紫外線損傷方面的抗氧化效果,本課題制備了輔酶Q10納米脂質(zhì)載體系統(tǒng)(CoQ10-NLC)。同時,對制備的載藥系統(tǒng)進(jìn)行初步的物理化學(xué)性質(zhì)的研究。利用激光散射儀、冷凍蝕刻電鏡和低溫冷凍電鏡等技術(shù)研究了CoQ10-NLC的粒徑、形貌和結(jié)構(gòu)等特征。 為更真實、準(zhǔn)確的模擬自然狀態(tài)的光老化,本課題采用人胚胎皮膚成纖維細(xì)胞(ESF-1)來建立體外細(xì)胞紫外損傷模型,ESF-1是一種原代培養(yǎng)的人皮膚細(xì)胞。對比添加普通CoQ10乳液組細(xì)胞,添加CoQ10-NLC的細(xì)胞在細(xì)胞活力、細(xì)胞形態(tài)和核形態(tài)方面都有明顯的提高和改善。此外,添加CoQ10-NLC的細(xì)胞丙二醛(MDA,細(xì)胞膜脂質(zhì)過氧化產(chǎn)物)水平只有添加CoQ10乳液組的61.5%。對比空白對照組,有CoQ10-NLC保護(hù)的細(xì)胞的超氧化物歧化酶(SOD)和谷胱甘肽過氧化物酶(GSH-px)活性分別提高了81%和75%。 在通過體外的細(xì)胞試驗研究CoQ10-NLC載藥系統(tǒng)在抗氧化方面的能力后,本課題又利用大鼠的背部皮膚作為模型來探討其體內(nèi)皮膚滲透性能。通過觀察熒光染料尼羅紅的特征熒光在皮膚縱切面上的分布來評估CoQ10-NLC克服皮膚屏障能力并在皮膚中的滲透效果,結(jié)果證明CoQ10-NLC較普通乳液具有更強(qiáng)的皮膚滲透能力。
[Abstract]:Skin photoaging is one of the important causes of skin aging. Sunlight ultraviolet rays mainly damage skin and cells by inducing the production of reactive oxygen free radicals and oxidative pressure. Coenzyme Q10 (CoQ10) is a potential antioxidant, but its insoluble properties limit its application in anti-UV induced photoaging of skin. NLCS is a new drug delivery system, which has the advantages of increasing drug dissolution ability, improving drug stability, enhancing skin permeability and reducing irritability. The main purpose of this paper is to design and prepare a novel transdermal nano-drug delivery system to carry CoQ10, and to study and evaluate its various functional properties in order to obtain an efficient, targeted and safe transdermal drug delivery system. In order to improve the antioxidation effect of CoQ10 in protecting skin from ultraviolet damage, CoQ10 nanometer lipid carrier system, CoQ10-NLCG, was prepared in this paper. At the same time, the physical and chemical properties of the drug loading system were studied. The particle size, morphology and structure of CoQ10-NLC were studied by means of laser scattering, freeze etching electron microscope and cryopreservation electron microscope. In order to simulate the photoaging of human embryonic skin fibroblasts (ESF-1), an in vitro ultraviolet damage model was established. ESF-1 is a primary cultured human skin cell. Compared with the normal CoQ10 emulsion group, the cell viability, cell morphology and nuclear morphology were significantly improved by adding CoQ10-NLC. In addition, the level of malondialdehyde (MDA), membrane lipid peroxidation product (MDAs) added with CoQ10-NLC was only 61.5 in CoQ10 emulsion group. Compared with the control group, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were increased by 81% and 75%, respectively. After studying the antioxidation ability of CoQ10-NLC drug carrier system in vitro, the rat back skin was used as a model to study the skin permeability in vivo. The distribution of characteristic fluorescence of fluorescent dye Nile red on the longitudinal surface of skin was observed to evaluate the ability of CoQ10-NLC to overcome the skin barrier and its permeation effect in the skin. The results showed that CoQ10-NLC had stronger skin permeability than ordinary emulsion.
【學(xué)位授予單位】：清華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R758.1

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