發(fā)布時間：2018-04-27 03:44

  本文選題：尋常型銀屑病 + 細胞因子　； 參考：《廣州中醫(yī)藥大學(xué)》2011年碩士論文

【摘要】：背景 銀屑病是皮膚科的常見病、多發(fā)病,男女均可發(fā)病,其確切的發(fā)病機制尚未完全闡明,涉及遺傳、免疫、炎癥、神經(jīng)介質(zhì)、細胞增殖與凋亡等多種因素。目前多數(shù)學(xué)者認為,銀屑病是一種免疫誘導(dǎo)、過度再生性增殖性皮膚病。超抗原的刺激、T淋巴細胞、T細胞與KC相互作用、細胞因子異常、STAT3途徑、免疫細胞膜分子異常、Toll受體以及神經(jīng)免疫等在銀屑病發(fā)病中起著重要作用。 目的 初步探討銀屑病患者血清中MCP-1、MIP-1α、SCD40L、TNF-α、EGF、IL-8、IL-10、IL-17及VEGF水平與中醫(yī)證型、疾病嚴重程度、疾病分期間的關(guān)系。 方法 采用酶聯(lián)免疫吸附方法(ELISA)對62例銀屑病患者和20例健康人血清中細胞因子(MCP-1、MIP-1α、SCD40L、TNF-α、EGF、IL-8、IL-10、VEGF)進行測定,并對其與各中醫(yī)證型的關(guān)系進行分析。 結(jié)果 1.EGF呈高表達,與健康對照組比較,無統(tǒng)計學(xué)意義(P0.05)；中醫(yī)各證型間比較,無統(tǒng)計學(xué)意義(P0.05)；與疾病分期、PASI評分無關(guān)(P0.05)；與病程有關(guān)(P0.01)。 2.IL-8呈高表達,血瘀證組最具代表意義,血瘀證組與健康對照組、血燥證組與健康對照組比較,有顯著性差異(P0.01)；與病情活動、PASI評分、病程無關(guān)(P0.05)。 3.IP-10呈高表達,血瘀證組最具代表意義,血瘀證組與健康對照組、血燥證組與健康對照組比較,有顯著性差異(P0.01)；與年齡、性別有關(guān)(P0.05)；與疾病分期、PASI評分、病程無關(guān)(P0.05)。 4. MCP-1呈高表達,與健康對照組比較,無統(tǒng)計學(xué)差異(P0.05)；血瘀證組與健康對照組比較,有統(tǒng)計學(xué)意義(P0.05)；與性別有關(guān)(P0.05)；與疾病分期、PAS I評分、病程無關(guān)(P0.05)。 5. MIP-1α呈高表達,與健康對照組比較有顯著性差異(P0.01)；血瘀證組與健康對照組、血燥證組與健康對照組比較有顯著性差異(P0.01)；與疾病分期、PASI評分、病程無關(guān)(P0.05)。 6. TNF-α呈高表達,與健康對照組比較有顯著性差異(P0.01)；血瘀證組與健康對照組、血燥證組與健康對照組比較有顯著性差異(P0.01)；與疾病分期、PASI評分、病程無關(guān)(P0.05)。 7. VEGF呈高表達,與健康對照組比較,無統(tǒng)計學(xué)意義(P0.05)；中醫(yī)辯各證型間比較,無統(tǒng)計學(xué)意義(P0.05)；與病程有關(guān)(P0.05)；與疾病分期、PASI評分無關(guān)(P0.05)。 8.IL-17呈低表達,與健康對照組比較,無統(tǒng)計學(xué)意義(P0.05)；中醫(yī)各證型間比較,無統(tǒng)計學(xué)意義(P0.05)；消退期與穩(wěn)定期、消退期與進行期間比較,有統(tǒng)計學(xué)意義(P0.01)；與PAS I評分、病程無關(guān)(P0.05)。 9. SCD40L呈低表達,血燥證組最具代表性,與健康對照組比較,有統(tǒng)計學(xué)意義(P0.05)；與疾病分期、PAS I評分、病程無關(guān)(P0.05)。 結(jié)論 1.EGF在銀屑病患者血清中高表達,且與病程有關(guān)。 2.IL-8在銀屑病患者血清中高表達,其檢測可能用于血瘀證、血燥證型銀屑病與其他證型銀屑病的中醫(yī)辯證分型。 3.IP-10在銀屑病患者血清中高表達,其檢測可能用于血瘀證、血燥證型銀屑病與其他證型銀屑病的中醫(yī)辯證分型,其表達水平與年齡、性別有關(guān)。 4.MCP-1在銀屑病患者血清中高表達,其檢測可能用于血瘀證型銀屑病與其他證型銀屑病的中醫(yī)辯證分型,其表達水平與性別有關(guān)。 5. MIP-1α在銀屑病患者血清中高表達,其檢測可能用于血瘀證、血燥證型銀屑病與其他證型銀屑病的中醫(yī)辯證分型。 6. TNF-α在銀屑病患者血清中高表達,其檢測可能用于血瘀證、血燥證型銀屑病與其他證型銀屑病的中醫(yī)辯證分型。 7. VEGF在銀屑病患者血清中高表達,與病程有關(guān)。 8.IL-17在銀屑病患者血清中低表達,與疾病活動有關(guān)。 9. SCD40L在銀屑病患者血清中低表達,其檢測可能用于血燥證型銀屑病與其他證型銀屑病的中醫(yī)辯證分型。 10.細胞因子表達異常在銀屑病的發(fā)病過程中起重要作用,異常的細胞因子間存在某些正向調(diào)控作用。
[Abstract]:background
Psoriasis is a common disease in the Department of dermatology. The pathogenesis of male and female is more common. The exact pathogenesis of psoriasis is not fully elucidated. It involves many factors, such as heredity, immunity, inflammation, nerve medium, cell proliferation and apoptosis. Most scholars believe that psoriasis is an immune induced, excessively regenerative proliferative dermatosis. Hyper antigen stimulation, T lymph nodes The interaction of cells, T cells with KC, abnormal cytokine, STAT3 pathway, immune cell membrane molecular abnormalities, Toll receptor and neuroimmunology play an important role in the pathogenesis of psoriasis.
objective
The relationship between the levels of MCP-1, MIP-1 a, SCD40L, TNF- a, EGF, IL-8, IL-10, IL-17 and VEGF in the serum of psoriasis patients with TCM syndrome, the severity of disease and the staging of disease.
Method
The serum levels of cytokines (MCP-1, MIP-1 a, SCD40L, TNF- a, EGF, IL-8, IL-10, VEGF) in 62 patients with psoriasis and 20 healthy people were measured by ELISA, and the relationship between them and TCM syndrome types was analyzed.
Result
The high expression of 1.EGF was not statistically significant (P0.05) compared with the healthy control group (P0.05), and there was no statistical significance (P0.05) in different TCM syndromes (P0.05); it was not related to the staging of the disease (P0.05), and related to the course of the disease (P0.01).
2.IL-8 showed high expression, blood stasis syndrome group was the most representative, blood stasis syndrome group and healthy control group, blood dryness syndrome group compared with healthy control group, there was significant difference (P0.01), and the disease activity, PASI score, the course of the disease was not related (P0.05).
3.IP-10 showed high expression, blood stasis syndrome group was the most representative, blood stasis syndrome group and healthy control group, blood dryness syndrome group compared with healthy control group, there was significant difference (P0.01), related to age, sex (P0.05), and disease staging, PASI score, and the course of disease (P0.05).
4. MCP-1 was highly expressed, and there was no statistical difference compared with the healthy control group (P0.05). The blood stasis syndrome group was statistically significant (P0.05) compared with the healthy control group (P0.05); it was related to sex (P0.05); the disease staging, PAS I score, and the course of disease were not related (P0.05).
5. MIP-1 a high expression, compared with the healthy control group has significant difference (P0.01), blood stasis syndrome group and healthy control group, blood dryness syndrome group and healthy control group have significant difference (P0.01), and the disease stage, PASI score, the course of the disease is not related (P0.05).
6. TNF- a high expression, compared with the healthy control group has significant difference (P0.01), blood stasis syndrome group and healthy control group, blood dryness syndrome group and healthy control group have significant difference (P0.01), and the disease stage, PASI score, the course of the disease is not related (P0.05).
The high expression of 7. VEGF was not statistically significant (P0.05) compared with the healthy control group (P0.05), and there was no statistical significance (P0.05) between the TCM syndromes and the course of disease (P0.05), and the PASI score was not related to the staging of the disease (P0.05).
8.IL-17 showed low expression, no statistical significance (P0.05) compared with the healthy control group (P0.05); there was no statistical significance (P0.05) for each type of TCM syndrome. The period of regression and stability, the period of regression and the period were statistically significant (P0.01), and the course of disease was not related with PAS I score (P0.05).
9. SCD40L showed low expression, the blood dryness syndrome was the most representative, compared with the healthy control group, it was statistically significant (P0.05), and the disease staging, PAS I score, and the course of disease were not related (P0.05).
conclusion
1.EGF is highly expressed in sera of psoriasis patients, and is related to the course of disease.
2.IL-8 is highly expressed in sera of psoriasis patients, and its detection may be used for blood stasis syndrome, psoriasis of blood dryness type syndrome and TCM syndrome differentiation of psoriasis.
3.IP-10 is highly expressed in the serum of patients with psoriasis, and its detection may be used in the dialectical typing of blood stasis syndrome, psoriasis of blood dryness syndrome and other syndromes of psoriasis. The expression level is related to age and sex.
The high expression of 4.MCP-1 in the serum of patients with psoriasis may be used in the dialectical typing of psoriasis of blood stasis syndrome and other syndromes of psoriasis. The expression level of psoriasis is related to sex.
5. MIP-1 alpha is highly expressed in sera of psoriasis patients, and its detection may be used for blood stasis syndrome, psoriasis of blood dryness syndrome and TCM syndrome differentiation of other syndromes.
6. TNF- alpha is highly expressed in sera of psoriasis patients, and its detection may be used for blood stasis syndrome, psoriasis of blood dryness syndrome and TCM syndrome differentiation of other syndromes.
7. high expression of VEGF in serum of psoriasis patients is related to the course of disease.
8.IL-17 is low in psoriatic serum and is associated with disease activity.
9. the expression of SCD40L in serum of psoriasis patients is low, and its detection may be used in TCM syndrome differentiation of psoriasis due to blood dryness syndrome and other syndromes.
10. abnormal expression of cytokines plays an important role in the pathogenesis of psoriasis. There are some positive regulatory effects between abnormal cytokines.

【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R758.63

【參考文獻】

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本文編號：1809060