本文選題：紫外線 + 皮膚鱗癌　； 參考：《昆明醫(yī)學(xué)院》2011年碩士論文

【摘要】：[目的]通過紫外線投照誘導(dǎo)裸鼠皮膚鱗癌,建立與人體頭面部皮膚鱗癌相似的動物模型,探索投照過程中所需紫外線的投照波長、劑量、時間。 [方法]取35只健康的Nu/Nu裸鼠、雌雄不限、3周齡、體重10g左右,隨機將其分為7組,給予不同波長、劑量的紫外線投照,分別為空白對照組、UVA(3.6J/cm2/day)、UVA(7.2 J/cm2/day)、UVB(720 mJ/cm2/day)、UVB(1440mJ/cm2/day)、UVA+UVB(3.6 J/cm2/day+720 mJ/cm2/day)、UVA+UVB(7.2 J/cm2/day+1440 mJ/cm2/day)。定期肉眼觀察裸鼠背部皮膚的變化,照射4周、8周、12周、16周后定時切取裸鼠背部局部病變皮膚組織,行常規(guī)病理檢查和免疫組織化學(xué)染色觀察裸鼠背部皮膚變化過程,分析皮膚組織中上皮角蛋白CK10、CK5/6表達強度,通過SPSS11.0軟件包采用單因素方差分析方法對其在不同時間位點的表達強度進行統(tǒng)計學(xué)分析。 [結(jié)果]空白對照組、UVA(3.6 J/cm2/day)、UVA(7.2 J/cm2/day)組肉眼觀察裸鼠皮膚無明顯異常變化,HE染色皮膚上皮層無明顯異常變化。UVB(720mJ/cm2/day)及UVA+UVB(3.6 J/cm2/day+720 mJ/cm2/day)組照射16周后裸鼠背部皮膚不斷增生、破潰,HE染色未見明顯癌變,上皮層表現(xiàn)為非典型增生、大量炎性細胞浸潤或局部潰瘍。UVB(1440mJ/cm2/day)及UVA+UVB(7.2 J/cm2/day+1440 mJ/cm2/day)照射16周后裸鼠背部皮膚明顯增生、破潰,形成火山口狀或乳頭狀增生HE染色表現(xiàn)為高分化鱗癌。CK5/6、CK10在UVA照射組中的表達強度和正常裸鼠皮膚基本一致,在UVB或UVA+UVB照射組的表達隨著照射劑量的增強、照射時間的增加,其陽性表達率逐漸增加。 [結(jié)論]①紫外線中的UVB是引起皮膚鱗癌的主要原因。②裸鼠皮膚鱗癌的發(fā)生與紫外線的投照波長、劑量及時間有關(guān)。③CK5/6、CK10在紫外線致皮膚損傷癌變的不同時間段表達強度不同,隨著照射劑量的增強、照射時間的增加,其陽性表達率逐漸增加。
[Abstract]:[objective] to establish an animal model similar to that of human head and face skin squamous cell carcinoma (SCC) induced by ultraviolet radiation in nude mice, and to explore the ultraviolet radiation wavelength, dose and time. [methods] Thirty-five healthy Nu/Nu nude mice were randomly divided into 7 groups. They were randomly divided into 7 groups. They were exposed to ultraviolet rays of different wavelengths and dosages, respectively, as the blank control group: UVA3.6J / cm ~ (-2) / cm ~ (2) J / cm ~ (2) / day UVB 720mJ / cm ~ (-1) 路cm ~ (-2) / day UVA UVB(3.6 J/cm2/day 720mJ / cm ~ (2) / day UVA UVB(7.2 J/cm2/day 1440 mJ / cm ~ 2r-1 / r ~ (-1) UVA UVB(7.2 J/cm2/day 1440 mg / cm ~ (-2) / r ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1)? The skin changes in the back of nude mice were observed with naked eye, and the skin tissues of local lesions in the back of nude mice were cut regularly after 4 weeks, 8 weeks, 12 weeks and 16 weeks of irradiation, and the process of skin changes in the back of nude mice was observed by routine pathological examination and immunohistochemical staining. The expression intensity of CK10 / CK5 / 6 in skin tissue was analyzed. The expression intensity of CK10 / CK5 / 6 in different time points was statistically analyzed by using single factor variance analysis (ANOVA) method with SPSS11.0 software package. [results] there was no obvious abnormal change in the skin of nude mice observed by naked eyes in the blank control group (UVA3. 6 J / cm 2 / 2 day) group. There was no obvious abnormal change in the epithelium of nude mice stained by HE. The skin of nude mice in the UVA UVB(3.6 J/cm2/day 720mJ / cm 2 / day group and UVA UVB(3.6 J/cm2/day 720mJ / cm 2 / day group were proliferated continuously after 16 weeks of irradiation. After 16 weeks of irradiation, the skin on the back of nude mice was obviously proliferated and ruptured. The epithelium showed atypical hyperplasia, a large number of inflammatory cell infiltration or local ulcers (1440mJ / cm ~ 2 / day) and UVA UVB(7.2 J/cm2/day 1440 mg / cm ~ (2) / day). The HE staining for the formation of volcanic or papillary hyperplasia showed that the expression of CK5 / 6CK10 in the UVA irradiation group was similar to that in the normal nude mice skin, and the expression in the UVB or UVA UVB group was increased with the increase of irradiation dose. Its positive expression rate increased gradually. [conclusion] 1 UVB in ultraviolet ray is the main cause of cutaneous squamous cell carcinoma in nude mice. The positive expression rate of CK5 / 6 CK10 was increased with the increase of dose and time, and the expression intensity of CK10 was different in the different time period of ultraviolet induced skin damage and carcinogenesis, with the increase of irradiation dose and irradiation time, the positive expression rate of CK10 was increased gradually.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R739.5

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