本文選題：微管不穩(wěn)定蛋白 + 慢性瘙癢　； 參考：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:通過炎癥性皮膚病患者及干皮性慢性瘙癢小鼠模型,研究微管不穩(wěn)定蛋白stathmin在慢性瘙癢發(fā)病機(jī)制中的作用。方法:收集35例炎癥性皮膚病引起的慢性瘙癢患者的皮損標(biāo)本和3例對(duì)照皮膚標(biāo)本。用免疫組化方法檢測(cè)皮膚活檢標(biāo)本中stathmin的表達(dá);用蘇木精-伊紅染色法檢測(cè)患者表皮組織中病理結(jié)構(gòu)的變化。采用丙酮-乙醚-水誘導(dǎo)的干皮性慢性瘙癢小鼠模型,小鼠的皮損、頸段及腰段脊髓和大腦處取材,通過免疫印跡和實(shí)時(shí)熒光定量PCR去檢測(cè)AEW模型組與對(duì)照組間stathmin的表達(dá)差異。結(jié)果:在炎癥性皮膚病患者中,與正常對(duì)照的皮膚相比,IHC顯示,stathmin在患者皮損中表皮角質(zhì)形成細(xì)胞、毛囊上皮細(xì)胞和免疫炎癥細(xì)胞中高表達(dá);HE染色顯示患者皮損處增厚,角化過度,并伴有炎癥性細(xì)胞浸潤(rùn)。在干皮性慢性瘙癢小鼠模型中,相對(duì)于對(duì)照組,免疫印跡顯示,stathmin在AEW組皮損中表達(dá)上調(diào),實(shí)時(shí)熒光定量PCR顯示,stathmin在AEW組皮損和大腦中表達(dá)上調(diào)。結(jié)論:研究結(jié)果初步表明stathmin在慢性瘙癢的機(jī)制中發(fā)揮作用。Stathmin通過在角質(zhì)形成細(xì)胞、上皮細(xì)胞和免疫炎癥細(xì)胞中的表達(dá)影響皮膚屏障功能,促進(jìn)慢性瘙癢的進(jìn)程。Stathmin可能是慢性瘙癢治療的新靶點(diǎn),對(duì)慢性瘙癢的治療和新藥的研發(fā)具有重要意義。
[Abstract]:Aim: to study the role of microtubule unstable protein stathmin in the pathogenesis of chronic pruritus in mice with inflammatory dermatosis and dry skin chronic pruritus. Methods: 35 cases of chronic pruritus caused by inflammatory dermatosis and 3 cases of control skin were collected. Immunohistochemical method was used to detect the expression of stathmin in skin biopsy specimens and hematoxylin-eosin staining was used to detect the pathological structure of epidermis. The mice model of chronic dry skin pruritus induced by acetone ether water was used to detect the difference of stathmin expression between the AEW model group and the control group by immunoblotting and real-time fluorescence quantitative PCR. Results: in the patients with inflammatory dermatosis, IHC showed that stathmin in epidermal keratinocytes, hair follicle epithelial cells and immune inflammatory cells showed thickening in the lesions of the patients compared with the normal skin. Hyperkeratosis accompanied by inflammatory cell infiltration. In the dry skin chronic pruritus mice model, the expression of stathmin was up-regulated in the skin lesions of AEW group compared with the control group, and the expression of stathmin was up-regulated in the skin lesions and cerebrum of AEW group by real-time quantitative PCR. Conclusion: the results suggest that stathmin plays a role in the mechanism of chronic pruritus. Stathmin affects the skin barrier function through its expression in keratinocytes, epithelial cells and immune inflammatory cells. Stathmin may be a new target for the treatment of chronic pruritus, which is of great significance for the treatment of chronic pruritus and the development of new drugs.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R758.31

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1 茹麗艷;微管不穩(wěn)定蛋白stathmin在慢性瘙癢中的作用[D];重慶醫(yī)科大學(xué);2017年

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