本文選題：claudinl蛋白　切入點：經(jīng)皮水分丟失　出處：《臨床皮膚科雜志》2014年06期 　論文類型：期刊論文

【摘要】：目的:觀察不同年齡階段健康人群曝光皮膚組織中緊密連接蛋白claudin1分子的表達情況,探討其與表皮屏障功能之間的關聯(lián)性,并分析慢性紫外線照射對皮膚組織中claudin1蛋白的表達及表皮屏障功能的影響。方法:收集不同年齡共39人曝光部位皮膚組織(包括頸項部、手背部或額面部),其中20歲組9例,20~50歲組14例,50歲組16例。分別以RT-PCR檢測皮膚組織中的claudin1分子轉錄情況,以免疫組化法檢測claudin1蛋白的表達,并檢測不同皮膚組織的經(jīng)皮水分丟失(TEWL)值。結果:RT-PCR檢測顯示cluadin1分子的轉錄水平均隨年齡增大而下調,50歲組claudin1分子的mRNA表達較20歲組明顯降低(P=0.0370)。免疫組化結果顯示曝光部位皮膚組織中claudin1蛋白的表達強度隨年齡增長而減弱,與年齡呈負相關,與RT-PCR結果一致。老年組(50歲組)曝光部位皮膚的TEWL值較中年組(20~50歲組)及青年組(20歲組)均升高,TEWL值與claudin1蛋白的表達呈負相關(P=0.0475)。結論:慢性紫外線照射可引發(fā)人曝光部位皮膚的屏障功能損傷,而這種屏障功能受損可能與claudin1蛋白的下調表達有關。
[Abstract]:Objective: to observe the expression of tight junction protein (claudin1) molecules in exposed skin tissues of healthy people at different ages, and to explore its relationship with the function of epidermal barrier. The effects of chronic ultraviolet irradiation on the expression of claudin1 protein and the function of epidermal barrier in skin tissue were analyzed. There were 9 cases of 20 years old group, 14 cases of 20 years old group and 16 cases of 50 years old group. RT-PCR was used to detect the transcription of claudin1 molecule in skin tissue, and the expression of claudin1 protein was detected by immunohistochemical method. Results the transdermal water loss (TEWL) values of different skin tissues were measured. Results the cluadin1 transcription level was down-regulated with age by RT-PCR. The mRNA expression of claudin1 in 50 years old group was significantly lower than that in 20 years old group. The immunohistochemical results showed that the mRNA expression of claudin1 molecule in 50 years old group was significantly lower than that in 20 years old group. The immunohistochemical results showed that the transcription level of claudin1 molecule decreased significantly. The expression of claudin1 protein in skin tissue of exposure site decreased with age. Negative correlation with age, The TEWL value of the exposed skin in the elderly group was higher than that in the middle age group (20 years old group) and the young group group (20 years old group). There was a negative correlation between the claudin1 protein expression and the expression of claudin1 protein. Conclusion: chronic ultraviolet radiation can induce human. Damage to skin barrier function at exposure site, The impairment of the barrier function may be related to the down-regulation of claudin1 protein.
【作者單位】： 浙江大學醫(yī)學院附屬第一醫(yī)院皮膚科;
【基金】：2010年中華醫(yī)學會-歐萊雅中國人健康皮膚研究基金(S2011080804) 浙江省衛(wèi)生廳科研基金(2009B045)資助項目
【分類號】：R751

【共引文獻】

相關期刊論文 前10條

1 李傳應;葛霞;;卵巢上皮性腫瘤中claudin3、claudin4的表達及其意義[J];安徽醫(yī)藥;2012年03期

2 陳磊;葛霞;;claudin的研究進展[J];蚌埠醫(yī)學院學報;2007年04期

3 紀小奇;張榮新;曹金林;朱金海;;claudin-3、4在食管鱗狀細胞癌中的表達及其臨床意義[J];蚌埠醫(yī)學院學報;2011年01期

4 陳yN招,立花修,長谷川光廣,徐如祥,山下純宏;Claudin-5(CLN5)在小腦成血管母細胞瘤中的表達和意義[J];中華神經(jīng)醫(yī)學雜志;2004年04期

5 王剛;吳小候;;Claudin-3在膀胱移行細胞癌中的表達及意義[J];重慶醫(yī)學;2009年17期

6 張中偉;秦環(huán)龍;;腸上皮細胞緊密連接的結構及功能研究進展[J];腸外與腸內營養(yǎng);2005年06期

7 張中偉;秦環(huán)龍;;乳酸菌對感染腸上皮細胞通透性及緊密連接蛋白表達的影響[J];腸外與腸內營養(yǎng);2007年04期

8 羅華銘;唐偉;郭毅;何r，

本文編號：1645297