本文選題：皮膚鱗狀細(xì)胞癌　切入點(diǎn)：基底細(xì)胞癌　出處：《鄭州大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 皮膚鱗狀細(xì)胞癌(squamous cell carcinoma, SCC)以及基底細(xì)胞癌(basal cell carcinoma, BCC)同源于表皮,共屬于非黑色素性腫瘤。臨床研究證實(shí),SCC轉(zhuǎn)移率較高,預(yù)后差,而B(niǎo)CC主要表現(xiàn)為局部破壞性浸潤(rùn),極少發(fā)生轉(zhuǎn)移,這兩種腫瘤生物學(xué)行為有較大差異。 目前很多研究都證明了VEGF及其受體在多數(shù)實(shí)體腫瘤組織中表達(dá)明顯增高,但是目前關(guān)于VEGFR-2在SCC中的表達(dá),及VEGFR-1與VEGFR-2在SCC與BCC組織中表達(dá)的差異和相關(guān)性研究目前鮮有文獻(xiàn)報(bào)道。本研究利用免疫組化SP法,定量檢測(cè)SCC, BCC及正常皮膚組織中VEGF, VEGFR-1, VEGFR-2三種蛋白的表達(dá),并比較其之間的差異性以及SCC組織中三種蛋白表達(dá)的相關(guān)性,來(lái)探討三者在非黑色素性皮膚癌的發(fā)生中所起的作用,從而為皮膚腫瘤的防治提供新的思路。 方法 收集手術(shù)切除的皮膚鱗狀細(xì)胞癌石蠟包埋標(biāo)本26例,其中高分化16例,中低分化10例；皮膚基底細(xì)胞癌標(biāo)本18例；取10例正常皮膚為對(duì)照組。采用免疫組化SP法檢測(cè)其中VEGF, VEGFR-1, VEGFR-2的表達(dá),三種蛋白的表達(dá)采用積分光密度值進(jìn)行分析,用已知的VEGF, VEGFR-1, VEGFR-2陽(yáng)性的皮肽鱗狀細(xì)胞癌及堆底細(xì)胞癌標(biāo)本做為陽(yáng)性刈‘j!{{,以PBS代替‘抗孵育組織切片作為陰性對(duì)照；結(jié)合臨床病理分級(jí),應(yīng)用SPSS17.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果 1. VEGF, VEGFR-1, VEGFR-2主要表達(dá)J：JJIll瘤實(shí)質(zhì)細(xì)J胞胞漿,農(nóng)皮』-I底層以上除角質(zhì)層外的細(xì)胞,部分血管內(nèi)J皮細(xì)胞可見(jiàn)5j5j表達(dá),為胞質(zhì)Il]棕黃色’染色顆粒。 2. VEGF在鱗狀細(xì)胞癌,基底細(xì)胞癌以及難常J皮膚·l]的秋分光街度值分別為(129.052±9.933),(116.067±3.864)以及(85.254±12.982),VEGFR-1在其三者中的表達(dá)水平為(130.481±12.020),(117.453±8.157)以及(82.193±9.150),VEGFR-2分別為(136.604±6.672),(121.535±7.499)以及(85.430±9.334)。VEGF, VEGFR-1, VEGFR-2在SCC組織[lll的陽(yáng)性表達(dá)與BCC及IIi常皮膚組的表達(dá)比較,均有顯著性差異(F=83.418,78.137以及169.125,P0.05),且征SCC組織中表達(dá)顯著i盞于BCC(P0.05)；BCC LflVEGF, VEGFR-1, VEGFR-2的表達(dá)與正常皮肽相比,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 3. VEGF在i高分化及中低分化鱗狀細(xì)胞癌中的積分光密度值分別為(124.492±8.969),(136.346±6.630)：VEGFR-1征高分化及l(fā)ll低分化鱗狀細(xì)胞癌中的積分光密度值分別為(127.076±13.616),(135.930±6.221)；VEGFR-2在高分化及中低分化鱗狀細(xì)胞癌中的積分光密度值分別為(133.573+_.6.563),(141.453±3.065)。VEGF及VEGFR-2在tIIf氐分化SCC中的表達(dá)顯著高于高分化SCC(P0.05),即分化越差,表達(dá)越高；高分化與中低分化SCC中的VEGFR-1表達(dá)比較,差異不具統(tǒng)計(jì)學(xué)意義(P0.05)。 4.在皮膚鱗狀細(xì)胞癌11],VEGF的表達(dá)程度與VEGFR-1成正相關(guān)1(r=0.773,P0.05),IjVEGFR-2的表達(dá)成II淵I關(guān)(r=0.676,P0.05),.IJVEGFR-1的表達(dá)水j川jVEGFR-2成_II淵j關(guān)(r=0.718,P0.05)。 結(jié)論 1.皮膚鱗狀細(xì)胞癌和基底細(xì)胞煽中,VEGF, VEGFR-1, VEGFR-2的蛋白表達(dá)水平可能�！生物’學(xué)特征相關(guān), i符-f門異常斯表達(dá)IIJ‘能為非黑色索性J皮膚癌過(guò)增殖的原因之。 2. VEGF, VEGFR-2的陽(yáng)性表達(dá)與SCC病理學(xué)分級(jí)呈負(fù)相關(guān),而VEGFR.1的陽(yáng)性表達(dá)與SCC組織學(xué)分級(jí)無(wú)明顯關(guān)系。 3. VEGF, VEGFR-1, VEGFR-2蛋白在SCC中的表達(dá)呈正相關(guān),三者可能共同促進(jìn)腫瘤的血管生成。 4. VEGF, VEGFR-1, VEGFR-2在皮膚鱗狀細(xì)胞癌和基底細(xì)胞癌的發(fā)生發(fā)展過(guò)程中可能起著重要作用,聯(lián)合檢測(cè)三者在非黑色素性皮膚癌中的表達(dá)可能作為腫瘤的診斷,預(yù)后判斷標(biāo)準(zhǔn)之一。
[Abstract]:objective
Cutaneous squamous cell carcinoma (squamous cell, carcinoma, SCC) and basal cell carcinoma (basal cell, carcinoma, BCC) homologous to the epidermis, belong to non melanoma tumors. Clinical studies have confirmed that SCC transfer rate is high, the prognosis is poor, while BCC is mainly manifested as local destructive infiltration, fewer metastases, two the biological behavior of the tumor are quite different.
At present, many studies have demonstrated that the expression of VEGF and its receptor in most solid tumor tissues were significantly increased, but the expression of VEGFR-2 in SCC in the study of differences and correlation between the expression of VEGFR-1 and VEGFR-2 in SCC and BCC tissues and there are few reports. This study used immunohistochemical SP method for quantitative detection of SCC. VEGF, BCC and VEGFR-1 in normal skin tissues, the expression of VEGFR-2 three protein, and three protein expression differences between the correlation and the SCC organization, to explore the three plays in the event of non melanoma skin cancer in the role, to provide new ideas for prevention and treatment of skin cancer.
Method
Cutaneous squamous cell carcinoma paraffin embedded specimens were collected from surgical operation in 26 cases, including 16 cases of high differentiation, 10 cases of low differentiated specimens; basal cell carcinoma in 18 cases; 10 cases of normal skin as control group. Immunohistochemical SP method was used to detect the VEGF, VEGFR-1, VEGFR-2 expression, expression of the three proteins the integral optical density values were analyzed using known VEGF, VEGFR-1, VEGFR-2 positive skin squamous cell carcinoma and basal cell carcinoma specimens as a heap of positive mow "J! PBS" instead of turning, with anti incubated tissue slices as negative control; combined with the clinical pathological grade, were analyzed by statistics software SPSS17.0.
Result
1. VEGF, VEGFR-1, VEGFR-2 mainly expressed J:JJIll cell parenchyma, fine J cytoplasm, agricultural skin, -I above the bottom layer, except for stratum corneum, some J cells in blood vessels showed 5j5j expression, cytoplasm Il] brown yellow staining granules.
2. VEGF in squamous cell carcinoma, basal cell carcinoma and often difficult to J l] street light skin equinox degree = (129.052 + 9.933), (116.067 + 3.864) and (85.254 + 12.982), the expression level of VEGFR-1 in three of the (130.481 + 12.020), (117.453 + 8.157) to and (82.193 + 9.150) VEGFR-2, respectively (136.604 + 6.672), (121.535 + 7.499) and (85.430 + 9.334).VEGF, VEGFR-1, compare the expression of VEGFR-2 in the positive expression of BCC and IIi and SCC [lll normal skin group, there were significant differences (F=83.418,78.137, 169.125, P0.05, SCC) and the I lamp syndrome expression was significantly in BCC (P0.05); BCC LflVEGF, VEGFR-1, VEGFR-2 expression was compared with normal skin, the difference was statistically significant (P0.05).
The integral optical density of 3. VEGF I in high differentiation and low differentiation squamous cell carcinoma respectively (124.492 + 8.969), (136.346 + 6.630):VEGFR-1 syndrome integral optical density high differentiation and low differentiation of LLL squamous cell carcinoma respectively (127.076 + 13.616), (135.930 + 6.221); integral light the density of VEGFR-2 in high differentiation and low differentiation squamous cell carcinoma respectively (133.573+_.6.563), (141.453 + 3.065) the expression of.VEGF and VEGFR-2 in tIIf di SCC differentiation was significantly higher than that of high differentiated SCC (P0.05), the poorer the differentiation, the higher the expression; expression of high differentiation and low differentiation in SCC VEGFR-1, the difference was not statistically significant (P0.05).
4., in skin squamous cell carcinoma 11], the expression level of VEGF is positively correlated with VEGFR-1, 1 (r=0.773, P0.05), and IjVEGFR-2 expression is II II I (r=0.676, P0.05), and the expression of.IJVEGFR-1 is water, Sichuan, Sichuan and y.
conclusion
1. skin squamous cell carcinoma and basal cell fans, VEGF, VEGFR-1, the expression level of VEGFR-2 protein may be "C". The biological characteristics of I, -f, abnormal expression of IIJ with the door "for non black J simply because of the proliferation of skin cancer too.
2. VEGF, the positive expression of VEGFR-2 was negatively correlated with the pathological grade of SCC, but the positive expression of VEGFR.1 was not significantly related to the histological grade of SCC.
The expression of 3. VEGF, VEGFR-1, and VEGFR-2 protein in SCC is positively correlated, and the three may jointly promote the angiogenesis of the tumor.
4. VEGF, VEGFR-1 and VEGFR-2 may play an important role in the occurrence and development of cutaneous squamous cell carcinoma and basal cell carcinoma. Combined detection of three expression in non melanoma skin cancer is probably one of the criteria for the diagnosis and prognosis of tumors.

【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R739.5

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