本文選題：慢性蕁麻疹　切入點(diǎn)：紅細(xì)胞　出處：《右江民族醫(yī)學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：慢性蕁麻疹的發(fā)病機(jī)制尚不清楚,免疫功能障礙是一個(gè)重要因素。紅細(xì)胞免疫是人體天然免疫的重要組成部分,許多疾病的發(fā)病機(jī)制與紅細(xì)胞免疫功能異常有關(guān)。現(xiàn)今國內(nèi)外對(duì)于慢性蕁麻疹紅細(xì)胞免疫功能相關(guān)的研究報(bào)告較少,且不夠深入,研究方法亦多采用傳統(tǒng)的紅細(xì)胞C3b受體(RBC-C3b)花環(huán)試驗(yàn)和紅細(xì)胞免疫復(fù)合物(RBC-IC)花環(huán)試驗(yàn),所獲得的實(shí)驗(yàn)結(jié)果不盡相同,但大多數(shù)報(bào)告的觀點(diǎn)是慢性蕁麻疹患者存在紅細(xì)胞免疫功能亢進(jìn)和紊亂�，F(xiàn)有研究表明,紅細(xì)胞作為機(jī)體血循環(huán)含量最多的血細(xì)胞,表達(dá)多種天然免疫受體和物質(zhì),如CR1、CR3、CD44、CD58、CD59、DAF、SOD酶、趨化因子受體等。在機(jī)體天然免疫反應(yīng)中及T淋巴細(xì)胞、B淋巴細(xì)胞特異免疫反應(yīng)中,紅細(xì)胞都占有重要地位,它參與了機(jī)體的免疫調(diào)控,對(duì)特異性免疫應(yīng)答中關(guān)于抗原選擇與應(yīng)答類型有指導(dǎo)作用。近年來,對(duì)系統(tǒng)性紅斑狼瘡、惡性腫瘤、肝炎等疾病的紅細(xì)胞免疫功能研究已取得了很大進(jìn)展,并發(fā)現(xiàn)大多數(shù)免疫相關(guān)性疾病的紅細(xì)胞免疫功能顯著降低。慢性蕁麻疹患者紅細(xì)胞免疫功能狀況究竟如何,紅細(xì)胞表面免疫分子的表達(dá)是否存在變化以及其在慢性蕁麻疹發(fā)病機(jī)制中的作用值得我們?nèi)ド钊胙芯�。本課題通過從不同方面、不同水平分別對(duì)慢性蕁麻疹患者紅細(xì)胞免疫功能進(jìn)行研究,包括紅細(xì)胞表面CD35、CD44、CD55、CD58、CD59等分子表達(dá)情況,以及紅細(xì)胞CR1密度相關(guān)基因的分布頻率,以探討紅細(xì)胞免疫在慢性蕁麻疹發(fā)病機(jī)制中的作用。對(duì)慢性蕁麻疹紅細(xì)胞免疫進(jìn)行深入研究將有助于對(duì)該病的全面理解,開辟新的途徑來了解該病的致病機(jī)制。目的:檢測慢性蕁麻疹患者紅細(xì)胞表面天然免疫分子(CD35、CD44、CD55、CD58、CD59)表達(dá)情況,了解慢性蕁麻疹患者紅細(xì)胞免疫狀況;以及慢性蕁麻疹患者紅細(xì)胞CR1密度相關(guān)基因多態(tài)性,探討慢性蕁麻疹患者紅細(xì)胞CR1密度相關(guān)基因型的頻率分布。方法:根據(jù)納入標(biāo)準(zhǔn)分別設(shè)正常對(duì)照組和慢性蕁麻疹組,抽取新鮮血液,用流式細(xì)胞儀定量檢測紅細(xì)胞表面CD35、CD44、CD55、CD58、CD59的平均熒光強(qiáng)度。提取研究對(duì)象DNA,采用聚合酶鏈?zhǔn)椒磻?yīng)-限制性片段長度多態(tài)性分析(PCR-RFLP)方法測定紅細(xì)胞CR1密度相關(guān)基因多態(tài)性。結(jié)果:(1)與對(duì)照組相比,慢性蕁麻疹患者組紅細(xì)胞CD35、CD55、CD58表達(dá)顯著升高(P0.05),CD44、CD59表達(dá)顯著降低(P0.05)。(2)慢性蕁麻疹組中CR1基因HH、HL和LL基因型頻率分別為74.2%、21.5%和4.3%,對(duì)照組HH、HL和LL基因型頻率分別為71.8%、23.6%和4.6%,兩組CR1基因型的分布頻率差異無顯著性(P0.05)。結(jié)論:(1)慢性蕁麻疹患者紅細(xì)胞CD35分子的數(shù)量較正常人增高,提示慢性蕁麻疹患者的紅細(xì)胞免疫功能存在亢進(jìn)和紊亂。紅細(xì)胞CD35分子數(shù)量增多可能是慢性蕁麻疹天然免疫反應(yīng)能力亢進(jìn)和紊亂的基礎(chǔ)。(2)慢性蕁麻疹紅細(xì)胞表面CD44表達(dá)降低,CD55、CD58、CD59表達(dá)升高,可能對(duì)慢性蕁麻疹的發(fā)病有一定影響。(3)慢性蕁麻疹患者紅細(xì)胞CD35數(shù)量的改變與CR1密度相關(guān)基因的遺傳關(guān)系不密切,可能是后天獲得性因素影響所致。
[Abstract]:The pathogenesis of chronic urticaria is unclear, immune dysfunction is an important factor. Red cell immunity is an important component of human innate immunity in the pathogenesis of many diseases, and erythrocyte immune function abnormalities. At home and abroad for chronic urticaria of red cell immune function related research report less, and in-depth, research methods also the traditional red cell C3b receptor rosette test (RBC-C3b) and red blood cell immune complex rosette test (RBC-IC), the experimental results are not the same, but most of the reported point of view is that the red cell immune function in patients with chronic urticaria and hyperactivity disorder exists. The existing research shows that red blood cells as the blood cells of the body most of the content of blood circulation, the expression of a variety of innate immune receptors and substances, such as CR1, CR3, CD44, CD58, CD59, DAF, SOD enzyme, chemokine receptor of innate immunity in the body. Immune response and T lymphocyte, B lymphocyte specific immune response, red blood cell plays an important role, it is involved in the immune regulation, the specific immune response of antigen selection and response type guiding role. In recent years, the systemic lupus erythematosus, malignant tumor, immunologic function of red blood cell hepatitis other diseases have made a great progress, and found that most of the red cell immune function in immune related diseases were significantly decreased. Function of red blood cells in patients with chronic urticaria by how the expression of surface of red blood cell immune molecules, whether there are changes and its role in the pathogenesis of chronic urticaria is worth researching. This paper from different aspects of different levels were studied on red cell immune function in patients with chronic urticaria, including red blood cell surface CD35, CD44, CD55, CD58, CD59 etc. The molecular expression, and the density of CR1 in red blood cell related gene frequency distribution, to explore the role of red blood cell immunity in the pathogenesis of chronic urticaria. The chronic urticaria of red cell immune in-depth study will contribute to a comprehensive understanding of the disease, open up a new way to understand the pathogenesis of this disease. Objective: to detect the the surface of red blood cells in patients with chronic urticaria of innate immune molecules (CD35, CD44, CD55, CD58, CD59) expression, understanding of red cell immune in patients with chronic urticaria; and chronic urticaria patients erythrocyte CR1 density related gene polymorphism, the frequency distribution of chronic urticaria patients erythrocyte CR1 density associated genotype. Methods: according to the the inclusion criteria were divided into normal group and chronic urticaria group, extraction of fresh blood were detected by flow cytometry and red cell surface CD35, CD44, CD55, CD58, CD59 average fluorescence Study on the extraction of strength. The object DNA, using restriction fragment length polymorphism analysis of polymerase chain reaction (PCR-RFLP) method for the determination of erythrocytes CR1 genomic density polymorphism. Results: (1) compared with the control group, patients with chronic urticaria group of red blood cell CD35, CD55, CD58 expression was significantly increased (P0.05), CD44, CD59 the expression was significantly decreased (P0.05). (2) CR1 gene HH in chronic urticaria group, HL and LL genotype frequencies were 74.2%, 21.5% and 4.3% in control group, HH, HL and LL genotype frequencies were 71.8%, 23.6% and 4.6%, the difference distribution of CR1 genotype frequency in the two groups had no significant (P0.05). Conclusion: (1) the number of red blood cells in patients with chronic urticaria CD35 molecules than in the normal population increased, suggesting that the red cell immune function in patients with chronic urticaria are hyperfunction and disorder. Increase in the number of red blood cell CD35 molecule may be chronic urticaria natural immune response ability of hyperfunction and disorder (2) chronic urticaria. The surface of red blood cells decreased expression of CD44, CD55, CD58, CD59 expression increased, may have an effect on the incidence of chronic urticaria. (3) do not close genetic relationship between CR1 gene density changes associated with the number of red blood cells CD35 in patients with chronic urticaria, can be acquired by influencing factors.

【學(xué)位授予單位】：右江民族醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R758.24

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 周挺;王婧;;銀屑病患者紅細(xì)胞參數(shù)變化及其臨床意義分析[J];檢驗(yàn)醫(yī)學(xué)與臨床;2016年14期

2 丁寧;;系統(tǒng)性紅斑狼瘡患者的紅細(xì)胞免疫狀態(tài)變化觀察[J];中國現(xiàn)代藥物應(yīng)用;2016年12期

3 趙瑞芹;付海燕;張麗霞;徐梅先;王鳳英;殷春蘭;安會(huì)波;;紅細(xì)胞免疫粘附功能及CR1基因多態(tài)性對(duì)危重型手足口病的預(yù)警作用[J];河北醫(yī)藥;2015年24期

4 何敬遠(yuǎn);;病毒性乙型肝炎患者紅細(xì)胞免疫功能檢測及其分析[J];中國藥物經(jīng)濟(jì)學(xué);2015年09期

5 林建;郭聶濤;;慢性腎衰竭患者紅細(xì)胞補(bǔ)體受體1型分子及其基因多態(tài)性變化[J];包頭醫(yī)學(xué)院學(xué)報(bào);2015年06期

6 胡金川;田亞平;田薇薇;溫新宇;高艷紅;王玲;董洪方;李巖;;紅細(xì)胞補(bǔ)體受體1單核苷酸多態(tài)性與肝細(xì)胞癌發(fā)病風(fēng)險(xiǎn)的研究[J];臨床腫瘤學(xué)雜志;2015年06期

7 賈成;郭宜姣;袁東亞;;高原環(huán)境下漢族人群免疫改變[J];中華臨床免疫和變態(tài)反應(yīng)雜志;2014年03期

8 郎曉東;;不同年齡健康人紅細(xì)胞免疫功能的變化分析[J];中國當(dāng)代醫(yī)藥;2014年07期

9 胡金川;田亞平;江朝光;溫新宇;高艷紅;董矜;郭廣宏;王玲;董振南;;紅細(xì)胞補(bǔ)體受體1分子水平及其基因單核苷酸多態(tài)性與系統(tǒng)性紅斑狼瘡的關(guān)聯(lián)研究[J];醫(yī)學(xué)研究生學(xué)報(bào);2013年07期

10 陳文成;農(nóng)樂根;韋葉生;韋瑩慧;王俊利;黃衍強(qiáng);黃燕;;桂西地區(qū)少數(shù)民族瘧疾感染人群紅細(xì)胞CD35分子表達(dá)研究[J];右江醫(yī)學(xué);2013年03期

相關(guān)會(huì)議論文 前1條

1 羅穎;晏洪波;楊李;;慢性蕁麻疹患者紅細(xì)胞CR1分子表達(dá)及外周血IgE、C3、C4水平的相關(guān)性研究[A];2012全國中西醫(yī)結(jié)合皮膚性病學(xué)術(shù)會(huì)議論文匯編[C];2012年

相關(guān)碩士學(xué)位論文 前5條

1 王韻瓊;慢性蕁麻疹與血清IL-22相關(guān)性研究[D];廣西醫(yī)科大學(xué);2015年

2 王娜;CD35和C4bp在兒童紫癜性腎炎中的表達(dá)及意義[D];鄭州大學(xué);2015年

3 劉洋;癲癇患兒紅細(xì)胞免疫功能與紅細(xì)胞補(bǔ)體受體1密度相關(guān)基因多態(tài)性的關(guān)系[D];吉林大學(xué);2011年

4 白明杰;慢性蕁麻疹患者紅細(xì)胞免疫功能初步研究[D];青島大學(xué);2005年

5 劉輝;銀屑病患者紅細(xì)胞免疫功能研究[D];第二軍醫(yī)大學(xué);2002年

，

本文編號(hào)：1631853