本文選題：亞臨床型生殖器皰疹排毒　切入點(diǎn)：IL-2　出處：《桂林醫(yī)學(xué)院》2010年碩士論文　論文類型：學(xué)位論文

【摘要】：目的探討亞臨床型生殖器皰疹患者脫排病毒與患者機(jī)體免疫狀態(tài)的相關(guān)性。 方法收集2009年1-11月我校附屬醫(yī)院皮膚性病科門診亞臨床型生殖器皰疹(Subclinical genital herpes,Subclinical GH)患者60名,用熒光定量聚合酶鏈?zhǔn)椒磻?yīng)(Fluorescence quantitative Polymerase Chain Reaction ,FQ-PCR)測定病毒脫排情況,并用酶聯(lián)免疫吸附劑試驗(yàn)(enzyme linked immunosorbent assay,ELISA)測定患者血清白介素2(interleukin-2,IL-2)、干擾素γ(interferon-γ,IFN-γ)水平和血清抗體。采用PEMS3.1統(tǒng)計軟件進(jìn)行相關(guān)分析。 結(jié)果60例亞臨床型生殖器皰疹患者標(biāo)本中有23例單純皰疹病毒核酸檢測呈陽性反應(yīng),陽性率為38.33%。亞臨床型生殖器皰疹患者組血清IL-2水平明顯低于正常對照組,差異有顯著性(t=27.0499,p0.05);患者組血清IFN-γ水平明顯低于正常對照組,差異有顯著性(t=10.2462,p0.05)。亞臨床型生殖器皰疹患者脫排病毒陽性組血清IL-2水平明顯低于陰性組,差異有顯著性(t’=11.0866,p0.05);脫排病毒陽性組患者血清IFN-γ水平明顯低于陰性組,差異有顯著性(t’=10.1519,p0.05)。60例亞臨床型生殖器皰疹患者血清抗體陽性率為71.67%,其中血清抗體陽性組脫排病毒陽性率為30.23%,明顯低于血清抗體陰性組(58.82%),差異有顯著性(χ2=4.2131,p=0.040.05);血清抗體陽性組血清IL-2水平明顯高于血清抗體陰性組,差異有顯著性(t =3.5741,p0.05);血清抗體陽性組血清IFN-γ水平明顯高于血清抗體陰性組,差異有顯著性(t’=5.1005,p0.05)。 結(jié)論1、亞臨床型生殖器皰疹患者盡管無明顯臨床癥狀,但存在無癥狀脫毒現(xiàn)象,是生殖器皰疹一個重要的傳染源,應(yīng)予以高度重視。2、亞臨床型生殖器皰疹患者中,血清抗體陽性組脫排病毒陽性率明顯低于血清抗體陰性組,并且血清抗體陽性組血清IL-2、IFN-γ明顯高于血清抗體陰性組,說明生殖器皰疹血清抗體的產(chǎn)生對限制病毒的復(fù)制有一定的作用。3、亞臨床型生殖器皰疹患者血清IL-2、IFN-γ水平低于正常人群血清水平,并且生殖器皰疹病毒脫排陽性組患者血清IL-2、IFN-γ水平低于皰疹病毒脫排陰性組患者,提示亞臨床型生殖器皰疹患者細(xì)胞免疫功能低下,在一定條件下容易激發(fā)致病。
[Abstract]:Objective to investigate the relationship between the immune status of patients with subclinical genital herpes. Methods A total of 60 patients with subclinical genital herpes herpes were collected from 2009 to November in the Department of Dermatology and venereal Disease, affiliated Hospital of our University. The virus demotion was determined by fluorescence quantitative polymerase chain reaction (FQPCR) and fluorescence quantitative polymerase chain reaction (FQ-PCR). The serum levels of interleukin-2 interleukin-2 interleukin-2 and interferon- 緯 interferon- 緯 (IFN- 緯) and serum antibodies were determined by enzyme linked immunosorbent assaysa. The correlation was analyzed by PEMS3.1 software. Results among the 60 cases of subclinical genital herpes, 23 cases of herpes simplex virus nucleic acid showed positive reaction, the positive rate was 38.33. The serum IL-2 level of patients with subclinical genital herpes was significantly lower than that of normal control group. The serum level of IFN- 緯 in the patients group was significantly lower than that in the normal control group (P 0.05). The serum IL-2 level in the patients with subclinical genital herpes was significantly lower than that in the negative group, and the level of serum IFN- 緯 in the patients with subclinical herpes genitalis was significantly lower than that in the negative group. The level of IFN- 緯 in the patients with positive apoplexy virus was significantly lower than that in the negative group, there was a significant difference between the two groups, and the level of IFN- 緯 in the positive group was significantly lower than that in the negative group. The positive rate of serum antibody in patients with subclinical herpes genitalis was 71.677.The positive rate of serum antibody positive group was 30.23 and was significantly lower than that of serum-negative group (蠂 24.2131p0.040.05), and the positive rate of serum antibody positive was 30.23g, significantly lower than that of serum-negative group (蠂 24.2131p0.040.05). The serum IL-2 level in sex group was significantly higher than that in serum antibody negative group. The level of IFN- 緯 in serum antibody positive group was significantly higher than that in serum antibody negative group, and there was significant difference in serum IFN- 緯 level between serum antibody positive group and serum antibody negative group. Conclusion 1. Although subclinical genital herpes patients have no obvious clinical symptoms, but there is asymptomatic detoxification phenomenon, it is an important source of infection for genital herpes. The positive rate of serum antibody positive group was significantly lower than that of serum antibody negative group, and the serum IL-2 IFN- 緯 level in serum antibody positive group was significantly higher than that in serum antibody negative group. The results showed that the production of serum antibodies to genital herpes had a certain effect on limiting the replication of the virus, and the serum levels of IL-2mIFN- 緯 in patients with subclinical genital herpes were lower than those in normal subjects. The serum levels of IL-2mIFN- 緯 in patients with genital herpes virus (HSV) positive were lower than those in patients with HSV negative, suggesting that the cellular immune function of patients with subclinical herpes genitalis was low, and it was easy to cause disease under certain conditions.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R752.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 傅錦堅(jiān);林元藻;胡立勇;;單純皰疹病毒疫苗的研究進(jìn)展[J];廣東藥學(xué)院學(xué)報;2008年01期

2 李新生,陳西成;局部注射重組α-2b干擾素合用伐昔洛韋治療男性復(fù)發(fā)性生殖器皰疹的療效觀察[J];中國麻風(fēng)皮膚病雜志;2005年09期

3 王復(fù)昆;馬黎民;;復(fù)發(fā)性生殖器皰疹患者免疫細(xì)胞檢測分析[J];中國麻風(fēng)皮膚病雜志;2006年08期

4 陳建華;鐘文俊;吳蕊;唐吉云;;生殖器皰疹患者血清IFN-γ、IL-2、IL-4和IL-10水平檢測及意義[J];中國麻風(fēng)皮膚病雜志;2007年11期

5 田中偉;宋向鳳;馮捷;;不同病程生殖器皰疹患者外周血Th1/Th2亞群分布的變化及其臨床意義[J];免疫學(xué)雜志;2006年01期

6 樊尚榮;;2006年美國疾病控制中心生殖器皰疹治療方案[J];中國全科醫(yī)學(xué);2006年17期

7 陶娟,陳興平,陳映玲;復(fù)發(fā)性生殖器皰疹患者外周血T細(xì)胞亞群及Th1/Th2型細(xì)胞因子的模式[J];咸寧醫(yī)學(xué)院學(xué)報;2002年02期

8 劉恩讓;張書嶺;王福喜;謝倩;劉姝麗;;兩種伐昔洛韋抑制療法方案預(yù)防生殖器皰疹復(fù)發(fā)作用比較[J];中國醫(yī)院用藥評價與分析;2007年04期

9 鄧云華,萬沐芬,楊東亮,陳興平,陳映玲;復(fù)發(fā)性生殖器皰疹患者外周血Tc1/Tc2亞群檢測及意義[J];中國皮膚性病學(xué)雜志;2005年04期

10 李偉;趙進(jìn);黃培勇;;轉(zhuǎn)移因子聯(lián)合阿昔洛韋治療頻發(fā)性生殖器皰疹療效觀察[J];中國皮膚性病學(xué)雜志;2008年03期

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