本文關(guān)鍵詞： 氟化鈉(NaF) 人黑素瘤細胞株A375 增殖活性　出處：《遵義醫(yī)學(xué)院》2010年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的：研究不同濃度氟化鈉(NaF)對體外培養(yǎng)的人黑素瘤細胞株A375(A375細胞株)增殖活性的影響,探索NaF對人惡性黑色素瘤(MM)的治療作用。方法：黑素瘤A375細胞株體外培養(yǎng)24h和48h后,用不同NaF(10,100、500、1000、5000、10000、20000與40000umol/L)處理,四甲基偶氮噻唑藍(MTT)測定NaF對A375細胞株增殖活性的影響,倒置顯微鏡觀察細胞形態(tài)學(xué)變化。結(jié)果：NaF為10 umol/L時,促進黑素瘤細胞的增殖。從100umol/L開始,隨著藥物濃度的提高和作用時間的延長,黑素瘤細胞的存活率降低,抑制率提高。但10000、20000與40000umol/L三組作用A375細胞24h,對細胞的增殖影響沒有區(qū)別(p0.05)；20000與40000umol/L兩組作用48h,對細胞的增殖影響也沒有區(qū)別(p0.05)。100與500umol/L表現(xiàn)為抑制作用,大于500umol/L則出現(xiàn)細胞毒性作用,表現(xiàn)為細胞數(shù)目明顯減少,細胞脫壁,變圓變小,胞膜邊界不清,胞核消失等變化。結(jié)論：NaF對A375細胞株的增殖活性具有雙向調(diào)節(jié)作用,即低濃度促進細胞增殖,高濃度殺傷和抑制細胞增殖。NaF對A375細胞株增殖活性的影響作用呈劑量-時間-效應(yīng)關(guān)系。
[Abstract]:Objective: to study the effect of sodium fluoride (NAF) at different concentrations on the proliferation of human melanoma cell line A375 (A375) in vitro, and to explore the therapeutic effect of NaF on human malignant melanoma. The effects of NaF on the proliferation of A375 cell line were measured with different NAF 10, 10, 100, 500, 000, 500, 000, 000, 000, 000 and 400 mol / L treatments. The morphologic changes of A375 cell line were observed by inverted microscope. Results when NAF was 10 umol/L, the proliferation of melanoma cells was promoted. With the increase of drug concentration and the prolongation of action time, the survival rate of melanoma cells decreased. The inhibition rate of A375 cells was increased in the three groups of 10000mg / L and 40000umolr / L for 24 h, but there was no difference in the proliferation of A375 cells between the two groups (p 0.05 / L and 40000umolr / L) for 48 h, and the effects on the proliferation of A375 cells were not different between the three groups (P 0.05N 路100 and 500umolr / L), but the cytotoxic effects were observed when the cells were larger than 500umolrL. The results showed that the cell number decreased, the cell dewalling, the circle became smaller, the cell membrane boundary was unclear, and the nucleus disappeared. Conclusion the cell proliferation activity of A375 cell line can be regulated by WNAF in both directions, that is, the low concentration of NAF can promote the proliferation of A375 cell line. The effects of high concentration of killing and inhibiting cell proliferation. NAF on the proliferation activity of A375 cell line showed a dose-time effect relationship.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.5

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