本文關(guān)鍵詞： 梅毒螺旋體 大環(huán)內(nèi)酯類抗生素 點(diǎn)突變　出處：《實(shí)用醫(yī)學(xué)雜志》2017年15期 　論文類型：期刊論文

【摘要】：目的調(diào)查湖南衡陽地區(qū)梅毒螺旋體23 rRNA基因上A2058G和A2059G突變現(xiàn)狀。方法常規(guī)PCR擴(kuò)增一期梅毒患者下疳樣本中Tp pol A基因,篩選Tp DNA陽性樣本;巢氏PCR擴(kuò)增Tp 23r RNA基因,分別采用限制性內(nèi)切酶MboⅡ和BsaⅠ酶切純化的PCR產(chǎn)物。結(jié)果 43例下疳分泌物中篩選出39例Tp DNA陽性樣本;39例樣本全部檢出A2058G突變,而未檢出A2059G突變。結(jié)論湖南衡陽地區(qū)Tp流行株存在高頻率23rRNA基因點(diǎn)突變,臨床上在治療梅毒時(shí)應(yīng)謹(jǐn)慎使用大環(huán)內(nèi)酯類抗生素。
[Abstract]:Objective to investigate the status of A2058G and A2059G mutations in 23 rRNA gene of Treponema pallidum in Hengyang, Hunan Province. Methods the TP pol A gene was amplified by routine PCR from the chancre samples of primary syphilis patients, and the positive samples of TP DNA were screened out, and the Tp23r RNA gene was amplified by nested PCR. The purified PCR products were digested by restriction endonuclease Mbo 鈪，

本文編號(hào)：1545729