本文關(guān)鍵詞： 光老化 紫外線 甲基蓮心堿　出處：《中國皮膚性病學(xué)雜志》2017年08期 　論文類型：期刊論文

【摘要】：目的探討甲基蓮心堿抗光老化的作用機(jī)制,為防治皮膚光老化性疾病提供實驗依據(jù)。方法建立中長波紫外線輻射人皮膚成纖維細(xì)胞光老化模型,實驗分為對照組、模型組(單純UVA+UVB照射)和藥物組(UVA+UVB照射后加0.4μmol/L,0.8μmol/L,1.6μmol/L甲基蓮心堿干預(yù)),檢測細(xì)胞內(nèi)ROS的水平和SOD和GPx的活力及MDA的含量。結(jié)果與模型組相比,甲基蓮心堿可以降低ROS水平及MDA含量(P0.05),同時提高SOD及GPx的活力(P0.05)。結(jié)論適當(dāng)濃度的甲基蓮心堿能降低光老化細(xì)胞內(nèi)ROS引起的氧化損傷,增加細(xì)胞內(nèi)抗氧化酶的活性,減輕細(xì)胞膜脂質(zhì)過氧化作用,延緩了皮膚光老化的進(jìn)程。
[Abstract]:Objective to investigate the anti-photoaging mechanism of methylliensine and to provide experimental evidence for the prevention and treatment of skin photoaging diseases. Methods A photoaging model of human skin fibroblasts irradiated by medium and long wavelength ultraviolet radiation was established and divided into two groups. Model group (simple UVA UVB irradiation) and drug group were treated with 0.4 渭 mol 路L ~ (-1) UVB and 0.4 渭 mol / L ~ (0.8) 渭 mol / L ~ (1.6) mol/L, respectively. The level of ROS, the activity of SOD and GPx, and the content of MDA in the cells were measured, and the results were compared with those in the model group. Methylliensine can decrease the level of ROS and the content of MDA and increase the activity of SOD and GPx. Conclusion the suitable concentration of MML can decrease the oxidative damage induced by ROS and increase the activity of antioxidant enzymes in the cells. It can reduce the lipid peroxidation of cell membrane and delay the process of skin photoaging.
【作者單位】： 西安交通大學(xué)第一附屬醫(yī)院;
【基金】：陜西省自然科學(xué)基金(2014JQ4151)
【分類號】：R751
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本文編號：1499705