波動(dòng)性甘油三酯水平及與高糖聯(lián)合培養(yǎng)對人臍靜脈內(nèi)皮細(xì)胞的損傷作用
發(fā)布時(shí)間：2019-07-09 07:02
【摘要】：目的:通過體外研究模擬餐后甘油三酯異常以及伴有的血糖異常對人臍靜脈內(nèi)皮細(xì)胞(human umbilical vein endothelial cell,HUVEC)的損傷作用,探討餐后狀態(tài)下甘油三酯、甘油三酯波動(dòng)、高血糖并存對血管內(nèi)皮的損傷作用。方法:1以人臍靜脈內(nèi)皮細(xì)胞(HUVEC)為研究對象,體外培養(yǎng)至第六代并應(yīng)用不含血清的1640完全培養(yǎng)基饑餓24小時(shí)已達(dá)到同步化后用于試驗(yàn)。2從醫(yī)院收集人的正常血清及高甘油三酯血清(HTGBS)并配比為所需濃度,并進(jìn)行除菌、滅活處理。其他指標(biāo)配比為相近濃度。血清中的甘油三酯(TG)、膽固醇(TC)、葡萄糖(GLU)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)等指標(biāo)均由醫(yī)院檢驗(yàn)科全自動(dòng)生化分析儀檢測。分裝并儲(chǔ)存在-80℃冰箱中。3實(shí)驗(yàn)共10組,給予以下條件,共作用3天。1組:葡萄糖濃度5.5 mmol/L+TG 1.56 mmol/L。2組:葡萄糖濃度5.5 mmol/L+TG 3.45 mmol/L,3組:葡萄糖濃度5.6 mmol/L+TG 5.52 mmol/L,4組:葡萄糖濃度5.5 mmol/L+TG 1.56 mmol/L(8小時(shí))與3.45 mmol/L(16小時(shí))交替,5組:葡萄糖濃度5.5 mmol/L+TG 1.56 mmol/L(8小時(shí))與5.52 mmol/L(16小時(shí))交替,6組:葡萄糖濃度25 mmol/L+TG 1.56 mmol/L,7組:葡萄糖濃度25 mmol/L+TG 3.45 mmol/L,8組:葡萄糖濃度25 mmol/L+TG 5.52 mmol/L,9組:葡萄糖濃度25 mmol/L+TG 1.56 mmol/L(8小時(shí))與3.45 mmol/L(16小時(shí))交替,10組:葡萄糖濃度25 mmol/L+TG 1.56 mmol/L(8小時(shí))與5.52 mmol/L(16小時(shí))交替。結(jié)束后換用葡萄糖濃度5.5 mmol/L+TG 1.56 mmol/L培養(yǎng)8小時(shí),測定各組細(xì)胞活力(MTT)指標(biāo),收取上清液測單核細(xì)胞趨化蛋白(monocyte chemoattractant protein-1,MCP-1)、血管內(nèi)皮細(xì)胞間粘附分子vascular cell adhesion molecule,VCAM-1)、細(xì)胞間粘附分子(interscular cell adhesionmolecule,ICAM)、C反應(yīng)蛋白(C-reactive protein,CRP)、丙二醛malondiadehyde,MDA)、超氧化物歧化酶(superoxide sismutase,SOD)、一氧化氮(nitric oxide,NO)。4統(tǒng)計(jì)學(xué)分析:符合正態(tài)分布計(jì)量資料以x±s表示,采用SPSS13.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析,多組間數(shù)據(jù)比較用單因素方差分析,兩兩比較用LSD檢驗(yàn),P0.05為差異有統(tǒng)計(jì)學(xué)意義。結(jié)果:1 MTT:中濃度甘油三酯波動(dòng)組大于高濃度波動(dòng)組的OD值(P0.05)。相同葡萄糖濃度情況下,與低濃度甘油三酯組比較,持續(xù)中濃度甘油三酯組OD值升高,而持續(xù)高濃度甘油三酯組OD值降低。在相同的甘油三酯作用下,高糖組要比低糖組的OD值低,單純高糖組的OD值大于單純高濃度甘油三酯組而小于單純中濃度高甘油三酯組,而高濃度葡萄糖與高濃度甘油三酯聯(lián)合培養(yǎng)組含量最低。同等程度的甘油三酯波動(dòng),高糖組的OD值要低于低糖組。2上清液CRP水平:相同葡萄糖濃度下,持續(xù)高甘油三酯及波動(dòng)性高甘油三脂組測得CRP均數(shù)均高于低甘油三酯組。在低葡萄糖濃度組,與低濃度甘油三酯組相比,其他各組CRP均升高,甘油三酯波動(dòng)組較等水平的高甘油三脂組低,但無明顯差別。在聯(lián)合高濃度葡萄糖組中,與低濃度甘油三脂組相比,其他各組CRP升高,但波動(dòng)性甘油三酯組較其無明顯差異。在同等甘油三酯水平下,高糖組CRP較低糖組高。單純高糖組CRP較單純高濃度甘油三酯組的CRP低,而高濃度葡萄糖與高濃度甘油三酯聯(lián)合培養(yǎng)組含量最高。同等程度的甘油三酯波動(dòng),高糖組的CRP含量要大于低糖組。3上清液ICAM含量:相同葡萄糖濃度下,持續(xù)高甘油三酯及波動(dòng)性高甘油三脂組測得ICAM均數(shù)均高于低甘油三酯組。在低葡萄糖濃度組,波動(dòng)性中濃度甘油三酯組與低濃度甘油三酯組相比無明顯差異,其他各組ICAM與其相比均明顯升高。在聯(lián)合高濃度葡萄糖組中,與低濃度甘油三脂組相比,持續(xù)高甘油三脂組ICAM均數(shù)升高,但波動(dòng)性甘油三酯組較其無明顯差異。在同等甘油三酯水平下,高糖組ICAM較低糖組高。單純高糖組ICAM較單純高濃度甘油三酯組的ICAM低,而高濃度葡萄糖 與高濃度甘油三酯聯(lián)合培養(yǎng)組含量最高。同等程度的甘油三酯波動(dòng),高糖組的ICAM含量要大于低糖組。4上清液VCAM含量:相同葡萄糖濃度下,持續(xù)高甘油三酯及波動(dòng)性高甘油三脂組測得VCAM均數(shù)均高于低甘油三酯組。在低葡萄糖濃度組,波動(dòng)性高濃度甘油三酯組和持續(xù)高濃度甘油三酯組與低濃度甘油三酯組相比明顯升高,其他各組VCAM與其相比無明顯差異。在聯(lián)合高濃度葡萄糖組中,與低濃度甘油三脂組相比,波動(dòng)性中濃度甘油三酯組較其無明顯差異,而其他組較其明顯升高。在同等甘油三酯水平下,高糖組CVAM較低糖組高。單純高糖組VCAM較單純高濃度甘油三酯組的VCAM低,而高濃度葡萄糖與高濃度甘油三酯聯(lián)合培養(yǎng)組含量最高。同等程度的甘油三酯波動(dòng),高糖組的VCAM含量要大于低糖組。5上清液MCP-1含量:相同葡萄糖濃度下,持續(xù)高甘油三酯及波動(dòng)性高甘油三脂組測得MCP-1均數(shù)均高于低甘油三酯組,波動(dòng)性高濃度甘油三酯組和持續(xù)高濃度甘油三酯組與低濃度甘油三酯組相比明顯升高,其他組MCP-1與其相比無明顯差異。在高濃度葡萄糖組中,持續(xù)高濃度甘油三酯組與低濃度甘油三酯組比較明顯升高,其他組較其無明顯差異。在同等甘油三酯水平下,高糖組MCP-1較低糖組高。單純高糖組MCP-1較單純高濃度甘油三酯組低,而高濃度葡萄糖與高濃度甘油三酯聯(lián)合培養(yǎng)組含量最高。同等程度的甘油三酯波動(dòng),高糖組的MCP-1含量要大于低糖組。6上清液MDA含量:相同葡萄糖濃度下,持續(xù)高甘油三酯及波動(dòng)性高甘油三脂均可不同程度增加上清液中炎性產(chǎn)物MDA的表達(dá)。但波動(dòng)性甘油三脂組較其無明顯差異。在同等甘油三酯水平下,高糖組MDA較低糖組高。單純高糖組MDA較單純高濃度甘油三酯組低,而高濃度葡萄糖與高濃度甘油三酯聯(lián)合培養(yǎng)組含量最高。同等程度的甘油三酯波動(dòng),高糖組的MDA含量要大于低糖組。7在相同葡萄糖濃度下,持續(xù)高甘油三酯及波動(dòng)性高甘油三脂均可降低上清液中保護(hù)性因子SOD、NO含量,在低葡萄糖濃度組,各組與低濃度甘油三酯組相比明顯降低。在聯(lián)合高濃度葡萄糖組中,與低濃度甘油三脂組相比降低,但波動(dòng)性高濃度甘油三酯組和持續(xù)高濃度甘油三酯組較其明顯降低。在同等甘油三酯水平下,高糖組NO和SOD較低糖組低。單純高糖組NO、SOD較單純高濃度甘油三酯組高,而高濃度葡萄糖與高濃度甘油三酯聯(lián)合培養(yǎng)組含量最低。同等程度的甘油三酯波動(dòng),高糖組的SOD、NO含量要大于低糖組。結(jié)論:1在相同葡萄糖濃度時(shí),波動(dòng)性甘油三酯和持續(xù)中濃度甘油三酯均促進(jìn)細(xì)胞增殖,且低糖并中濃度甘油三酯波動(dòng)的促增殖作用最大,持續(xù)高濃度甘油三酯均抑制細(xì)胞增殖,且高糖聯(lián)合持續(xù)高濃度甘油三酯的抑制細(xì)胞增殖的作用最強(qiáng)。單純高濃度甘油三脂對細(xì)胞增殖的抑制作用大于單純高糖。2波動(dòng)性高甘油三酯和持續(xù)高甘油三酯對人臍靜脈內(nèi)皮細(xì)胞均有炎性損傷,但波動(dòng)性高甘油三酯對細(xì)胞的損傷不大于持續(xù)高甘油三酯。單純高水平的高甘油三脂對細(xì)胞的損傷作用大于單純高糖,高糖聯(lián)合持續(xù)高濃度的甘油三酯對細(xì)胞的作用最大,同等甘油三酯波動(dòng)時(shí),高葡萄糖的炎性損傷作用大于低葡萄糖。3波動(dòng)性高甘油三酯和持續(xù)高甘油三酯導(dǎo)致人臍靜脈內(nèi)皮細(xì)胞抗氧化能力減弱;但波動(dòng)性高甘油三酯對細(xì)胞抗氧化能力的影響不大于持續(xù)高甘油三酯。單純高水平的高甘油三脂對細(xì)胞的抗氧化能力的減弱作用大于單純高糖,高糖聯(lián)合持續(xù)高濃度的甘油三酯對細(xì)胞的抗氧化能力的減弱作用最大。同樣的甘油三酯波動(dòng)時(shí)時(shí),高葡萄糖的炎性損傷作用大于低葡萄糖。4波動(dòng)性高甘油三酯和持續(xù)高甘油三酯導(dǎo)致人臍靜脈內(nèi)皮細(xì)胞功能損傷(NO含量降低),但波動(dòng)性高甘油三酯對細(xì)胞的影響不大于持續(xù)高甘油三酯。單純高水平的高甘油三脂對細(xì)胞內(nèi)皮功能的損傷作用大于單純高糖,高糖聯(lián)合持續(xù)高濃度的甘油三酯對HUVEC功能的損傷作用最大。同樣的甘油三酯波動(dòng)時(shí)時(shí),高葡萄糖對HUVEC的損傷作用大于低葡萄糖。
[Abstract]:Objective: To study the effects of postprandial triglyceride and abnormal blood glucose on human umbilical vein endothelial cell (HUVEC) by in vitro study. Methods:1 Human umbilical vein endothelial cell (HUVEC) was used as the research object. The cells were cultured in vitro to the sixth generation and the serum-free 1640 full-medium starvation was applied for the test after 24 hours of synchronization. The normal serum and high triglyceride serum (HTGBS) of the human were collected from the hospital and matched to the desired concentration, and the sterilization and inactivation treatment were carried out. The proportion of other indexes is similar concentration. The indexes of triglyceride (TG), cholesterol (TC), glucose (GLU), low density lipoprotein (LDL) and high-density lipoprotein (HDL) in the serum are detected by the full-automatic biochemical analyzer of the hospital laboratory. The concentration of glucose was 5.5 mmol/ L + TG 1.56 mmol/ L, and the glucose concentration was 5.5mmol/ L + TG 3.45mmol/ L, and the glucose concentration was 5.6 mmol/ L + TG 5.52 mmol/ L. The glucose concentration was 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours),5 groups: glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours),6 groups: glucose concentration of 25 mmol/ L + TG 1.56 mmol/ L,7 groups: glucose concentration of 25 mmol/ L + TG 3.45 mmol/ L,8 groups: glucose concentration of 25 mmol/ L + TG 5.52 mmol/ L,9 groups: The glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours), and the glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours). After the end, the cell viability (MTT) index was measured with the glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L for 8 hours, and the cell adhesion molecule (ICAM-1), the vascular endothelial cell adhesion molecule (VCAM-1) and the inter-cell adhesion molecule (ICAM) were collected. Statistical analysis of C-reactive protein (CRP), malondiadehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO). One-factor analysis of variance was used to compare the data among the groups, and the two comparisons were compared with the LSD, and the difference between the two groups was statistically significant. Results:1 MTT: The concentration of the medium-concentration triglyceride was higher than that of the high-concentration fluctuation group (P0.05). In that case of the same glucose concentration, the OD value of the continuous medium-concentration triglyceride group was increase while the OD value of the high-concentration triglyceride group was decreased in comparison with the low-concentration triglyceride group. Under the same triglyceride, the OD value of the high-sugar group was lower than that of the low-sugar group, the OD value of the simple high-sugar group was higher than that of the pure high-concentration triglyceride group, and the content of the high-concentration glucose and the high-concentration triglyceride combined culture group was the lowest. In the same degree of triglyceride fluctuation, the OD value of the high-sugar group was lower than that of the low-sugar group. The CRP level of the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, compared with the low-concentration triglyceride group, the levels of CRP in the other groups increased, and the triglyceride fluctuation group was lower than that of the higher triglyceride group, but there was no significant difference. In the combined high-concentration glucose group, the CRP in other groups increased compared with the low-concentration triglyceride group, but there was no significant difference in the volatile triglyceride group. In that same triglyceride level, the CRP in the high-sugar group was higher than that of the low-sugar group. The CRP of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the high-concentration glucose combined with high-concentration triglyceride was the highest. In the same degree of triglyceride fluctuation, the content of CRP in the high-sugar group was higher than that of the low-sugar group. The content of ICAM-1 in the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, there was no significant difference between the concentration triglyceride group and the low-concentration triglyceride group in the fluctuation, and the ICAM-1 in the other groups was significantly higher than that of the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference in the number of ICAM-1 in the continuous high-fat group compared with the low-concentration triglyceride group. At the same triglyceride level, the ICAM-1 of the high-sugar group was higher than that of the low-sugar group. The ICAM-1 of the simple high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of ICAM-1 in the high-sugar group was higher than that of the low-sugar group, and the content of VCAM in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, the volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly increased compared with the low-concentration triglyceride group, and the other groups of VCAM had no significant difference compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference between the low-concentration triglyceride group and the low-concentration triglyceride group, and the other group was significantly higher than that in the other group. In that same triglyceride level, the CVIAM of the high-sugar group was higher than that of the low-sugar group. VCAM of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride was the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. The volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group increased significantly compared with the low-concentration triglyceride group, and the other groups of the MCP-1 had no significant difference compared with the low-concentration triglyceride group. In the high-concentration glucose group, the high-concentration triglyceride group and the low-concentration triglyceride group were significantly increased, and there was no significant difference in other groups. In the same triglyceride level, the high sugar group MCP-1 was higher in the lower sugar group. In the simple high-sugar group, MCP-1 was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the supernatant of the supernatant was higher than that of the low-sugar group. However, there was no significant difference between the volatility and the fat group. In that same triglyceride level, the MDA in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. the content of MDA in the high-sugar group is higher than that of the low-sugar group under the same glucose concentration, and the content of the protective factor SOD and the NO in the supernatant can be reduced, and the content of the SOD and the NO in the low-glucose concentration group can be reduced, The groups are obviously reduced compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, the low-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly reduced compared to the low-concentration triglyceride group. In that same triglyceride level, the NO and SOD of the high-sugar group were lower than that of the low-sugar group. The content of NO and SOD in the pure high-sugar group was higher than that of the pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride combined culture group was the lowest. And the content of SOD and NO in the high-sugar group is higher than that of the low-sugar group. Conclusion:1 At the same glucose concentration, both the volatile triglyceride and the continuous middle-concentration triglyceride promote the proliferation of the cell, and the proliferation of the low-sugar and medium-concentration triglyceride is the largest, and the high-concentration triglyceride can inhibit the proliferation of the cells. And the effect of high glucose and high concentration of triglyceride in inhibiting cell proliferation is the strongest. The inhibitory effect of pure high-concentration triglyceride on the proliferation of human umbilical vein was higher than that of pure high-sugar. the only high-level high-fat-fat-to-cell damage action is greater than that of pure high-sugar and high-sugar and high-concentration triglyceride to the cell, and when the equivalent triglyceride fluctuates, The inflammatory injury of high glucose is greater than that of low glucose. The high triglyceride and persistent high triglyceride of the high glucose result in the decrease of the anti-oxidation ability of human umbilical vein endothelial cells, but the effect of the high triglyceride on the anti-oxidation ability of the cells is not more than the continuous high triglyceride. The effect of high-level high-fat-fat on the anti-oxidation ability of the cells is greater than that of pure high-sugar, high-sugar and high-concentration triglyceride to the anti-oxidation ability of the cells. in that same triglyceride fluctuation, the inflammatory injury of high glucose is great than that of low-glucose.4-volatile high-triglyceride and persistent high-triglyceride result in the functional damage of human umbilical vein endothelial cells (NO content decrease), However, that effect of volatile high triglyceride on the cell is not great than the persistent high triglyceride. The effect of high-level high-fat-fat on the function of the endothelial function of the HUVEC was higher than that of pure high-sugar, high-sugar and high-concentration triglyceride, and the damage to HUVEC was the most. In the same triglyceride fluctuation, the damage of high glucose to HUVEC was greater than that of low glucose.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R589
本文編號：2511953
[Abstract]:Objective: To study the effects of postprandial triglyceride and abnormal blood glucose on human umbilical vein endothelial cell (HUVEC) by in vitro study. Methods:1 Human umbilical vein endothelial cell (HUVEC) was used as the research object. The cells were cultured in vitro to the sixth generation and the serum-free 1640 full-medium starvation was applied for the test after 24 hours of synchronization. The normal serum and high triglyceride serum (HTGBS) of the human were collected from the hospital and matched to the desired concentration, and the sterilization and inactivation treatment were carried out. The proportion of other indexes is similar concentration. The indexes of triglyceride (TG), cholesterol (TC), glucose (GLU), low density lipoprotein (LDL) and high-density lipoprotein (HDL) in the serum are detected by the full-automatic biochemical analyzer of the hospital laboratory. The concentration of glucose was 5.5 mmol/ L + TG 1.56 mmol/ L, and the glucose concentration was 5.5mmol/ L + TG 3.45mmol/ L, and the glucose concentration was 5.6 mmol/ L + TG 5.52 mmol/ L. The glucose concentration was 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours),5 groups: glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours),6 groups: glucose concentration of 25 mmol/ L + TG 1.56 mmol/ L,7 groups: glucose concentration of 25 mmol/ L + TG 3.45 mmol/ L,8 groups: glucose concentration of 25 mmol/ L + TG 5.52 mmol/ L,9 groups: The glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours), and the glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours). After the end, the cell viability (MTT) index was measured with the glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L for 8 hours, and the cell adhesion molecule (ICAM-1), the vascular endothelial cell adhesion molecule (VCAM-1) and the inter-cell adhesion molecule (ICAM) were collected. Statistical analysis of C-reactive protein (CRP), malondiadehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO). One-factor analysis of variance was used to compare the data among the groups, and the two comparisons were compared with the LSD, and the difference between the two groups was statistically significant. Results:1 MTT: The concentration of the medium-concentration triglyceride was higher than that of the high-concentration fluctuation group (P0.05). In that case of the same glucose concentration, the OD value of the continuous medium-concentration triglyceride group was increase while the OD value of the high-concentration triglyceride group was decreased in comparison with the low-concentration triglyceride group. Under the same triglyceride, the OD value of the high-sugar group was lower than that of the low-sugar group, the OD value of the simple high-sugar group was higher than that of the pure high-concentration triglyceride group, and the content of the high-concentration glucose and the high-concentration triglyceride combined culture group was the lowest. In the same degree of triglyceride fluctuation, the OD value of the high-sugar group was lower than that of the low-sugar group. The CRP level of the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, compared with the low-concentration triglyceride group, the levels of CRP in the other groups increased, and the triglyceride fluctuation group was lower than that of the higher triglyceride group, but there was no significant difference. In the combined high-concentration glucose group, the CRP in other groups increased compared with the low-concentration triglyceride group, but there was no significant difference in the volatile triglyceride group. In that same triglyceride level, the CRP in the high-sugar group was higher than that of the low-sugar group. The CRP of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the high-concentration glucose combined with high-concentration triglyceride was the highest. In the same degree of triglyceride fluctuation, the content of CRP in the high-sugar group was higher than that of the low-sugar group. The content of ICAM-1 in the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, there was no significant difference between the concentration triglyceride group and the low-concentration triglyceride group in the fluctuation, and the ICAM-1 in the other groups was significantly higher than that of the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference in the number of ICAM-1 in the continuous high-fat group compared with the low-concentration triglyceride group. At the same triglyceride level, the ICAM-1 of the high-sugar group was higher than that of the low-sugar group. The ICAM-1 of the simple high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of ICAM-1 in the high-sugar group was higher than that of the low-sugar group, and the content of VCAM in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, the volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly increased compared with the low-concentration triglyceride group, and the other groups of VCAM had no significant difference compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference between the low-concentration triglyceride group and the low-concentration triglyceride group, and the other group was significantly higher than that in the other group. In that same triglyceride level, the CVIAM of the high-sugar group was higher than that of the low-sugar group. VCAM of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride was the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. The volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group increased significantly compared with the low-concentration triglyceride group, and the other groups of the MCP-1 had no significant difference compared with the low-concentration triglyceride group. In the high-concentration glucose group, the high-concentration triglyceride group and the low-concentration triglyceride group were significantly increased, and there was no significant difference in other groups. In the same triglyceride level, the high sugar group MCP-1 was higher in the lower sugar group. In the simple high-sugar group, MCP-1 was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the supernatant of the supernatant was higher than that of the low-sugar group. However, there was no significant difference between the volatility and the fat group. In that same triglyceride level, the MDA in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. the content of MDA in the high-sugar group is higher than that of the low-sugar group under the same glucose concentration, and the content of the protective factor SOD and the NO in the supernatant can be reduced, and the content of the SOD and the NO in the low-glucose concentration group can be reduced, The groups are obviously reduced compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, the low-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly reduced compared to the low-concentration triglyceride group. In that same triglyceride level, the NO and SOD of the high-sugar group were lower than that of the low-sugar group. The content of NO and SOD in the pure high-sugar group was higher than that of the pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride combined culture group was the lowest. And the content of SOD and NO in the high-sugar group is higher than that of the low-sugar group. Conclusion:1 At the same glucose concentration, both the volatile triglyceride and the continuous middle-concentration triglyceride promote the proliferation of the cell, and the proliferation of the low-sugar and medium-concentration triglyceride is the largest, and the high-concentration triglyceride can inhibit the proliferation of the cells. And the effect of high glucose and high concentration of triglyceride in inhibiting cell proliferation is the strongest. The inhibitory effect of pure high-concentration triglyceride on the proliferation of human umbilical vein was higher than that of pure high-sugar. the only high-level high-fat-fat-to-cell damage action is greater than that of pure high-sugar and high-sugar and high-concentration triglyceride to the cell, and when the equivalent triglyceride fluctuates, The inflammatory injury of high glucose is greater than that of low glucose. The high triglyceride and persistent high triglyceride of the high glucose result in the decrease of the anti-oxidation ability of human umbilical vein endothelial cells, but the effect of the high triglyceride on the anti-oxidation ability of the cells is not more than the continuous high triglyceride. The effect of high-level high-fat-fat on the anti-oxidation ability of the cells is greater than that of pure high-sugar, high-sugar and high-concentration triglyceride to the anti-oxidation ability of the cells. in that same triglyceride fluctuation, the inflammatory injury of high glucose is great than that of low-glucose.4-volatile high-triglyceride and persistent high-triglyceride result in the functional damage of human umbilical vein endothelial cells (NO content decrease), However, that effect of volatile high triglyceride on the cell is not great than the persistent high triglyceride. The effect of high-level high-fat-fat on the function of the endothelial function of the HUVEC was higher than that of pure high-sugar, high-sugar and high-concentration triglyceride, and the damage to HUVEC was the most. In the same triglyceride fluctuation, the damage of high glucose to HUVEC was greater than that of low glucose.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R589
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