【摘要】：目的目前鋅α2糖蛋白(ZAG)與高尿酸血癥(HUA)的相關性研究較少。文中旨在通過檢測ZAG的mRNA、蛋白表達量在桂西地區(qū)HUA人群和尿酸(SUA)正常的健康人群之間的表達差異,初步探討ZAG與HUA的相關性。方法收集2016年3月至2016年5月右江民族醫(yī)學院附屬醫(yī)院HUA患者74例為HUA組,選取同期74例體檢中心健康體檢者作為對照組。將受試者的血液標本進行血清制備及生化指標檢測、總RNA提取,采用實時熒光定量PCR檢測ZAG mRNA表達量,酶聯(lián)免疫吸附測定檢測血清ZAG蛋白表達量,進而分析2組ZAG的mRNA和蛋白表達差異。結果 HUA組血清ZAG mRNA、ZAG蛋白表達量明顯高于對照組[(13.201±3.433)vs(11.731±4.033);(6.163±1.779)vs(5.540±0.573),P0.05]。SUA與ZAG蛋白表達量(r=0.176)、BMI(r=0.161)、SBP(r=0.144)、DBP(r=0.250)、BUN(r=0.461)、CREA(r=0.649)、TG(r=0.212)呈正相關,與HDL(r=-0.333)呈負相關,均有統(tǒng)計學意義(P0.05)。結論 ZAG可能成為預防及治療HUA及其相關代謝性疾病的新靶點。
[Abstract]:Objective at present, there are few studies on the correlation between zinc 偽 2 glycoprotein (ZAG) and hyperuricemia (HUA). The purpose of this study was to detect the expression of mRNA, protein in ZAG between HUA population in western Guangxi and healthy people with normal uric acid (SUA), and to explore the relationship between ZAG and HUA. Methods from March 2016 to May 2016, 74 patients with HUA in the affiliated Hospital of Youjiang Medical College for nationalities were collected as HUA group, and 74 healthy persons in physical examination center were selected as control group. The blood samples of the subjects were prepared and biochemical indexes were detected, the total RNA was extracted, the expression of ZAG mRNA was detected by real-time fluorescence quantitative PCR, and the expression of ZAG protein in serum was detected by enzyme-linked immunosorbent assay (Elisa). The difference of mRNA and protein expression between the two groups was analyzed. Results the expression of serum ZAG mRNA,ZAG protein in HUA group was significantly higher than that in control group [(13.201 鹵3.433) vs (11.731 鹵4.033)]. Sua was positively correlated with ZAG protein expression (r 鈮，

本文編號：2504347