發(fā)布時間：2019-06-03 09:11

【摘要】：目的探討外源性ghrelin對糖尿病心肌病(DCM)大鼠心肌動作電位及瞬時外向鉀電流(Ito)的影響。方法雄性SD大鼠腹腔注射鏈脲佐菌素(60mg/kg)誘導(dǎo)Ⅰ型DCM模型,并采用全細(xì)胞膜片鉗技術(shù)觀察ghrelin對糖尿病大鼠心室肌細(xì)胞動作電位及瞬時外向鉀電流的影響。結(jié)果與DCM組比較,10~(-7) mmol/L ghrelin明顯延長DCM大鼠心室肌細(xì)胞5%動作電位時程(APD50)[(12.49±2.32)%;n=7,P=0.037]及APD90[(26.29±5.13)%;n=7,P=0.006];并明顯抑制Ito電流峰值,抑制率[(23.14±3.07)%;n=9,P=0.021]。結(jié)論外源性ghrelin抑制Ito電流而延長DCM大鼠心肌動作電位時程,參與糖尿病大鼠心臟電生理的重構(gòu)。
[Abstract]:Objective to investigate the effect of exogenous ghrelin on myocardial action potential and transient outward potassium current (Ito) in diabetic cardiomyopathy (DCM) rats. Methods Type I DCM model was induced by intraabdominal injection of streptozotocin (60mg/kg) in male SD rats. The effects of ghrelin on action potential and transient outward potassium current in ventricular myocytes of diabetic rats were observed by whole cell patch clamp technique. Results compared with DCM group, 10 ~ (- 7) mmol/L ghrelin significantly prolonged 5% action potential duration (APD50) of ventricular myocytes in DCM rats [(12.49 鹵2.32)%; n 鈮，

本文編號：2491827