【摘要】：目的探討肝細(xì)胞生長因子調(diào)節(jié)的酪氨酸激酶底物(HGS)對胰島素表達(dá)和分泌的影響。方法檢測HGS,胰島素和胰升血糖素在小鼠胰島中的分布。檢測不同濃度葡萄糖(5.5、11.1及16.7mmol/L)培養(yǎng)24h后NIT-1細(xì)胞HGS表達(dá)水平和胰島素的分泌。觀察siRNA沉默HGS表達(dá)后48h NIT-1細(xì)胞胰島素的表達(dá)和分泌的變化。結(jié)果 HGS免疫陽性細(xì)胞特異性分布于小鼠胰腺的胰島,并與胰島素共定位于胰島β細(xì)胞中,未見HGS和胰高血糖素陽性熒光雙標(biāo)細(xì)胞。不同濃度葡萄糖培養(yǎng)NIT-1細(xì)胞24h,5.5mmol/L組為(25.07±0.93)μIU/mg,11.1mmol/L組為(32.28±0.93)μIU/mg,16.7mmol/L組為(41.77±1.39)μIU/mg,NIT-1細(xì)胞分泌胰島素水平逐漸增加(P0.01)。各組HGS蛋白水平也隨葡萄糖濃度增加而逐漸增加(P0.05)。siRNA沉默HGS表達(dá)后NIT-1細(xì)胞內(nèi)胰島素的表達(dá)水平無明顯變化,但胰島素分泌水平減少(P0.05)。結(jié)論 HGS選擇性表達(dá)于胰島β細(xì)胞中,并調(diào)節(jié)胰島素的分泌。
[Abstract]:Objective to investigate the effect of hepatocyte growth factor regulated tyrosine kinase substrate (HGS) on insulin expression and secretion. Methods the distribution of HGS, insulin and glucagon in mouse islets was detected. The expression of HGS and insulin secretion in NIT-1 cells cultured with different concentrations of glucose (5.5, 11.11 and 16.7mmol/L) for 24 h were detected. To observe the changes of insulin expression and secretion in NIT-1 cells 48 hours after siRNA silencing HGS expression. Results HGS immunopositive cells were specifically distributed in the islets of mouse pancreas and co-located in islet 尾 cells with insulin. No HGS and glucagon positive fluorescent double labeled cells were found. NIT-1 cells cultured at different concentrations of glucose for 24 hours were (25.07 鹵0.93) 渭 IU/mg,11.1mmol/L and (31.77 鹵1.39) 渭 IU/mg, in 5.5 mmol / L group, (32. 28 鹵0. 93) 渭 IU/mg,16.7mmol/L group and (41.77 鹵1.39) 渭 IU/mg,. The level of insulin secreted by NIT-1 cells increased gradually (P 0.01). The level of HGS protein in each group also increased gradually with the increase of glucose concentration (P 0.05). After silencing HGS expression, the expression level of insulin in NIT-1 cells did not change significantly, but the level of insulin secretion decreased (P 0.05). Conclusion HGS is selectively expressed in islet 尾 cells and regulates insulin secretion.
【作者單位】： 貴州省遵義市第一人民醫(yī)院內(nèi)分泌科;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢中心醫(yī)院內(nèi)分泌科 分子診斷湖北省重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金青年項(xiàng)目(31400916) 湖北省自然科學(xué)基金(2014CFB219) 武漢市青年科技晨光計(jì)劃(2015071704011624) 貴州省衛(wèi)生廳科學(xué)技術(shù)基金(黔科合SY字[2013]3036)
【分類號】：R587.1

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