【摘要】：目的探討肝細(xì)胞生長(zhǎng)因子調(diào)節(jié)的酪氨酸激酶底物(HGS)對(duì)胰島素表達(dá)和分泌的影響。方法檢測(cè)HGS,胰島素和胰升血糖素在小鼠胰島中的分布。檢測(cè)不同濃度葡萄糖(5.5、11.1及16.7mmol/L)培養(yǎng)24h后NIT-1細(xì)胞HGS表達(dá)水平和胰島素的分泌。觀察siRNA沉默HGS表達(dá)后48h NIT-1細(xì)胞胰島素的表達(dá)和分泌的變化。結(jié)果 HGS免疫陽(yáng)性細(xì)胞特異性分布于小鼠胰腺的胰島,并與胰島素共定位于胰島β細(xì)胞中,未見(jiàn)HGS和胰高血糖素陽(yáng)性熒光雙標(biāo)細(xì)胞。不同濃度葡萄糖培養(yǎng)NIT-1細(xì)胞24h,5.5mmol/L組為(25.07±0.93)μIU/mg,11.1mmol/L組為(32.28±0.93)μIU/mg,16.7mmol/L組為(41.77±1.39)μIU/mg,NIT-1細(xì)胞分泌胰島素水平逐漸增加(P0.01)。各組HGS蛋白水平也隨葡萄糖濃度增加而逐漸增加(P0.05)。siRNA沉默HGS表達(dá)后NIT-1細(xì)胞內(nèi)胰島素的表達(dá)水平無(wú)明顯變化,但胰島素分泌水平減少(P0.05)。結(jié)論 HGS選擇性表達(dá)于胰島β細(xì)胞中,并調(diào)節(jié)胰島素的分泌。
[Abstract]:Objective to investigate the effect of hepatocyte growth factor regulated tyrosine kinase substrate (HGS) on insulin expression and secretion. Methods the distribution of HGS, insulin and glucagon in mouse islets was detected. The expression of HGS and insulin secretion in NIT-1 cells cultured with different concentrations of glucose (5.5, 11.11 and 16.7mmol/L) for 24 h were detected. To observe the changes of insulin expression and secretion in NIT-1 cells 48 hours after siRNA silencing HGS expression. Results HGS immunopositive cells were specifically distributed in the islets of mouse pancreas and co-located in islet 尾 cells with insulin. No HGS and glucagon positive fluorescent double labeled cells were found. NIT-1 cells cultured at different concentrations of glucose for 24 hours were (25.07 鹵0.93) 渭 IU/mg,11.1mmol/L and (31.77 鹵1.39) 渭 IU/mg, in 5.5 mmol / L group, (32. 28 鹵0. 93) 渭 IU/mg,16.7mmol/L group and (41.77 鹵1.39) 渭 IU/mg,. The level of insulin secreted by NIT-1 cells increased gradually (P 0.01). The level of HGS protein in each group also increased gradually with the increase of glucose concentration (P 0.05). After silencing HGS expression, the expression level of insulin in NIT-1 cells did not change significantly, but the level of insulin secretion decreased (P 0.05). Conclusion HGS is selectively expressed in islet 尾 cells and regulates insulin secretion.
【作者單位】： 貴州省遵義市第一人民醫(yī)院內(nèi)分泌科;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢中心醫(yī)院內(nèi)分泌科 分子診斷湖北省重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金青年項(xiàng)目(31400916) 湖北省自然科學(xué)基金(2014CFB219) 武漢市青年科技晨光計(jì)劃(2015071704011624) 貴州省衛(wèi)生廳科學(xué)技術(shù)基金(黔科合SY字[2013]3036)
【分類(lèi)號(hào)】：R587.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前9條

1 周渝;廖雪梅;;胰島素過(guò)敏致休克反應(yīng)1例[J];四川醫(yī)學(xué);2014年04期

2 王雅泉;陳德容;李英娟;;胰島素在急性缺血性腦卒中伴糖尿病治療中應(yīng)用[J];當(dāng)代醫(yī)學(xué);2014年12期

3 楊曦;;1型糖尿病患者外周血CD_4~+CD_(25)~+細(xì)胞數(shù)量及其對(duì)胰島素治療效果的影響[J];山東醫(yī)藥;2014年10期

4 周一軍,張錦,王佳賀;2型糖尿病大血管病變患者血清肝細(xì)胞生長(zhǎng)因子水平[J];中國(guó)臨床康復(fù);2005年15期

5 薛繼萍;石永兵;吳允孚;陳剛;;全身炎癥反應(yīng)綜合征患者血清肝細(xì)胞生長(zhǎng)因子水平的變化及其與腎功能的相關(guān)性[J];蘇州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2010年04期

6 陳陵;;矮妖綜合征[J];臨床薈萃;2007年14期

7 姜東紅;賈慶華;楊志華;董菊子;鄧芝云;李曉云;;肝細(xì)胞生長(zhǎng)因子對(duì)鉛致人腎小球系膜細(xì)胞損傷的保護(hù)效應(yīng)的機(jī)制[J];職業(yè)與健康;2013年21期

8 甘宇;于黎麗;李鐵男;鄭長(zhǎng)虹;;自體骨髓單個(gè)核細(xì)胞移植治療糖尿病下肢血管病變療效及其與血清肝細(xì)胞生長(zhǎng)因子的關(guān)系[J];中國(guó)實(shí)用內(nèi)科雜志;2010年03期

9 涂盛;徐烙，

本文編號(hào)：2485307