【摘要】：目的:觀察胰島素干預(yù)1型糖尿病(T1DM)大鼠前后,睪丸組織中脂聯(lián)素、脂聯(lián)素受體及其介導(dǎo)的信號因子、炎性細(xì)胞因子的表達(dá)情況,探討胰島素對T1DM大鼠睪丸組織脂聯(lián)素、脂聯(lián)素受體及其介導(dǎo)的信號轉(zhuǎn)導(dǎo)通路表達(dá)的影響。方法:30只清潔級SD雄性大鼠隨機(jī)分成正常對照組(A組,n=8)和糖尿病造模組(n=22),造模組大鼠采用一次性腹腔注射65mg/Kg STZ以制備T1DM模型。將成模的16只雄鼠隨機(jī)分為糖尿病對照組(B組,n=8)和糖尿病胰島素治療組(C組,n=8)。C組大鼠每日皮下注射精蛋白鋅胰島素3~5U/只,血糖控制于10.0mmol/L左右,A、B組大鼠予等量生理鹽水。于實(shí)驗(yàn)8周末留取血標(biāo)本,用于血生化指標(biāo)、胰島素(Ins.)及性激素(T、LH、FSH)水平檢測,然后處死動物,迅速取出雙側(cè)睪丸及附睪,計(jì)數(shù)睪丸重量、精子數(shù)量及活動率,測定睪丸脂聯(lián)素(Adiponectin)、脂聯(lián)素受體(Adipo R)、AMP依賴的蛋白激酶(AMPK)、蛋白激酶B(AKT)、內(nèi)皮型一氧化氮合酶(e NOS)、一氧化氮(NO)、白細(xì)胞介素-6(IL-6)和腫瘤壞死因子-α(TNF-α)的表達(dá)水平。統(tǒng)計(jì)采用LSD-t檢驗(yàn)和單因素方差分析。結(jié)果:1.與A組比較,B組T1DM大鼠睪丸組織出現(xiàn)顯著病理改變。2.與A組比較,B組T1DM大鼠的血糖、血脂、睪丸組織IL-6、TNF-α、P-AKT/AKT、e NOS和NO表達(dá)水平較A組顯著升高(P0.05)。3.與A組比較,B組T1DM大鼠的血清Ins.和Adiponectin、血清和睪丸組織T、睪丸重量、精子數(shù)量及活動率、組織Adiponectin及Adipo R1、P-AMPK/AMPK水平較A組顯著降低(P0.05)。4.胰島素治療能顯著逆轉(zhuǎn)上述各項(xiàng)指標(biāo)的變化。結(jié)論:1.T1DM可導(dǎo)致大鼠睪丸組織發(fā)生嚴(yán)重病理改變。2.糖尿病大鼠病變睪丸組織中Adiponectin、Adipo R、信號轉(zhuǎn)導(dǎo)因子AMPK、AKT、e NOS、NO及炎性細(xì)胞因子IL-6、TNF-α均發(fā)生相應(yīng)改變,可能在T1DM大鼠睪丸組織病損中發(fā)揮了重要作用。3.胰島素逆轉(zhuǎn)了上述指標(biāo)變化。胰島素可能通過調(diào)節(jié)Adiponectin、Adipo R介導(dǎo)的信號轉(zhuǎn)導(dǎo)通路減輕糖尿病大鼠睪丸組織損傷程度,發(fā)揮保護(hù)作用。
[Abstract]:Objective: to observe the expression of adiponectin, adiponectin receptor, signal factor and inflammatory cytokines in testis of type 1 diabetes mellitus (T1DM) rats before and after insulin intervention, and to explore the effect of insulin on adiponectin in testis of T1DM rats. Effects of adiponectin receptor and its signal transduction pathway on the expression of adiponectin receptor. Methods: thirty clean grade SD male rats were randomly divided into two groups: normal control group (group A, n = 8) and diabetic model group (n = 22). T1DM model was made by intraperitoneal injection of 65mg/Kg STZ at one time in the model group. Sixteen male rats were randomly divided into two groups: diabetic control group (group B, n = 8) and diabetic insulin treatment group (group C, n = 8). C). The rats were subcutaneously injected with protamine zinc insulin (3~5U/) every day, and the blood glucose was controlled around 10.0mmol/L, A. Group B rats were given the same amount of saline. Blood samples were collected at the end of experiment 8 to be used as biochemical index of blood, insulin (Ins.) The levels of sex hormones (T, LH, FSH) were measured, and then the animals were killed. Bilateral testes and epididymis were taken out quickly. The weight of testis, sperm count and motility were counted. The adiponectin (Adiponectin), adiponectin receptor (Adipo R), in testis was determined. Expression of AMP-dependent protein kinase (AMPK), protein kinase B (AKT), endothelial nitric oxide synthase (e NOS), nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor-偽 (TNF- 偽). LSD-t test and one-way ANOVA were used. Results: 1. Compared with group A, there were significant pathological changes in testicular tissue of T1DM rats in group B. Compared with group A, the levels of blood glucose, blood lipid, IL-6,TNF- 偽, pAKT / Akt, e-NOS and NO in T1DM rats in group B were significantly higher than those in group A (P0.05). Serum Ins. of T1DM rats in group B compared with group A And Adiponectin, serum and testicular tissue T, testicular weight, sperm quantity and motility, tissue Adiponectin, Adipo R1, PxAMPK and AMPK levels were significantly lower than those in group A (P0.05). Insulin therapy can significantly reverse the changes of the above indexes. Conclusion: 1.T1DM can cause severe pathological changes in testicular tissue of rats. The changes of Adiponectin,Adipo R, signal transduction factor AMPK,AKT,e NOS,NO and inflammatory cytokine IL-6,TNF- 偽 in testicular tissue of diabetic rats may play an important role in the pathological changes of testicular tissue in T1DM rats. 3. Insulin reversed these changes. Insulin may play a protective role by modulating the signal transduction pathway mediated by Adiponectin,Adipo R to reduce the degree of testicular injury in diabetic rats.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R587.1

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