【摘要】：目的觀察白樺脂醇和地塞米松共同作用于小鼠MC3T3-E1細(xì)胞后,內(nèi)源性脂質(zhì)蓄積與細(xì)胞凋亡中的關(guān)系。方法以MC3T3-E1細(xì)胞為研究對(duì)象,采用白樺脂醇和地塞米松共同作用于MC3T3-E1細(xì)胞,測(cè)定細(xì)胞內(nèi)甘油三酯含量;NileRed熒光染色觀察脂質(zhì)蓄積情況;流式細(xì)胞技術(shù)測(cè)定成骨細(xì)胞凋亡率;ELISA法檢測(cè)Caspase-3酶活性;采用Spearman等級(jí)相關(guān)進(jìn)行脂質(zhì)含量與成骨細(xì)胞凋亡相關(guān)性分析。結(jié)果白樺脂醇和地塞米松共同作用于小鼠MC3T3-E1細(xì)胞后,0.5μmol/L地塞米松(B組)與1.0μmol/L地塞米松組(C組)較空白組(A組),細(xì)胞凋亡率、Caspase-3酶活性均明顯增高(P0.05),細(xì)胞內(nèi)甘油三酯含量明顯增加。2.0μg/ml白樺脂醇作用細(xì)胞后,地塞米松所致的細(xì)胞凋亡率上升與Caspase-3酶活性升高受到明顯抑制,同時(shí)細(xì)胞內(nèi)的甘油三酯蓄積降低。Spearman等級(jí)相關(guān)分析表明地塞米松誘導(dǎo)后的成骨細(xì)胞內(nèi)脂質(zhì)含量與細(xì)胞凋亡率呈正相關(guān),r=0.412,P0.05。結(jié)論白樺脂醇可顯著減輕地塞米松誘導(dǎo)的成骨細(xì)胞內(nèi)脂質(zhì)蓄積,降低細(xì)胞凋亡率;提示地塞米松所致細(xì)胞凋亡至少部分與細(xì)胞內(nèi)脂質(zhì)蓄積有關(guān)。
[Abstract]:Aim to investigate the relationship between endogenous lipid accumulation and apoptosis in MC3T3-E1 cells treated with Bai Hua fatty alcohol and dexamethasone. Methods MC3T3-E1 cells were treated with Bai Hua fatty alcohol and dexamethasone to determine the intracellular triglyceride content, NileRed fluorescence staining was used to observe the accumulation of lipid. The apoptosis rate of osteoblasts was measured by flow cytometry, the activity of Caspase-3 enzyme was measured by ELISA method, and the correlation between lipid content and apoptosis of osteoblasts was analyzed by Spearman grade correlation. Results after the MC3T3-E1 cells were treated with Bai Hua fatty alcohol and dexamethasone, 0.5 渭 mol / L dexamethasone (group B) and 1.0 渭 mol / L dexamethasone group (group C) were more effective than those of control group (group A), and the apoptosis rate was higher than that of control group (group A). The activity of Caspase-3 enzyme was significantly increased (P0.05) and the content of triglyceride in the cells was significantly increased. After treated with 2.0 渭 g / ml Bai Hua lipid alcohol, the increase of apoptosis rate and the increase of Caspase-3 enzyme activity induced by dexamethasone were significantly inhibited. Spearman rank correlation analysis showed that the lipid content in osteoblasts induced by dexamethasone was positively correlated with the apoptosis rate (r = 0.412, P 0.05). Conclusion Bai Hua fatty alcohol can significantly reduce the lipid accumulation and apoptosis rate of osteoblasts induced by dexamethasone, suggesting that the apoptosis induced by dexamethasone is at least partly related to the accumulation of intracellular lipid.
【作者單位】： 廣東醫(yī)科大學(xué)附屬醫(yī)院骨科中心;
【基金】：廣東省科技計(jì)劃項(xiàng)目(2012B031800490;2011B031800172)
【分類號(hào)】：R580
，

本文編號(hào)：2446085