【摘要】：目的:探討維生素C治療百草枯(paraquat,PQ)中毒大鼠的療效及量效關(guān)系。方法:將40只SpragueDawley(SD)大鼠隨機(jī)分為對照組、中毒組和維生素C1組、C2組,每組10只。通過胃內(nèi)一次性灌注150 mg/kg PQ,建立PQ中毒SD大鼠模型。中毒組給予30 mg/kg甲基強(qiáng)的松龍、2.5 mg/kg環(huán)磷酰胺腹腔注射;維生素C1組、C2組在中毒組基礎(chǔ)上給予兩種濃度的維生素C(5,500 mg/kg),以生理鹽水稀釋成5 m L/kg,股靜脈注射;對照組僅以生理鹽水進(jìn)行相應(yīng)處理。于實驗后36 h檢測大鼠血漿中丙二醛、肝腎功能,并行動脈血氣分析,采血后處死大鼠取肺組織行病理切片、濕干重比等檢查;取肝組織行比色法檢測谷胱甘肽過氧化物酶(glutathione peroxidase,GSH-PX),Western印跡檢測Bcl-2表達(dá)等。結(jié)果:維生素C1組中丙二醛低于中毒組,GSH-PX高于中毒組,肝腎功能較中毒組有明顯改善(均P0.01);維生素C2組丙二醛高于中毒組,其肝腎功能較中毒組也呈惡化趨勢(均P0.01)。中毒組中Bcl-2較對照組表達(dá)減低,維生素C1組中Bcl-2較中毒組表達(dá)增強(qiáng),而維生素C2組中Bcl-2較中毒組表達(dá)減弱(均P0.01)。但維生素C1組、C2組在肺功能、濕干重比、病理學(xué)等與中毒組差異無統(tǒng)計學(xué)意義(均P0.05)。結(jié)論:低劑量維生素C可能通過抗氧化、抑制PQ導(dǎo)致的細(xì)胞凋亡等途徑對PQ中毒大鼠的肝腎功能有部分的保護(hù)作用,而高劑量維生素C反而有促氧化作用;同時,維生素C未呈現(xiàn)出對PQ中毒時肺的保護(hù)作用。
[Abstract]:Objective: to investigate the efficacy of vitamin C in the treatment of paraquat (paraquat,PQ) poisoning in rats. Methods: 40 SpragueDawley (SD) rats were randomly divided into three groups: control group, intoxication group, vitamin C 1 group and C 2 group, with 10 rats in each group. The rat model of PQ poisoning SD was established by intragastric infusion of 150 mg/kg PQ,. In the poisoning group, 30 mg/kg methylprednisolone was injected intraperitoneally with 2.5 mg/kg cyclophosphamide. Two concentrations of vitamin C (5500 mg/kg) were given to vitamin C group and C2 group, diluted into 5 mL 路kg ~ (- 1) with saline and injected intravenously into femoral vein, while the control group was treated with normal saline only. Malondialdehyde (MDA) in plasma and function of liver and kidney were measured at 36 h after the experiment, and blood gas analysis was performed. Lung tissue was sacrificed after blood collection for pathological section and wet-dry weight ratio (WW / W). The expression of glutathione peroxidase (Bcl-2) in liver tissue was detected by glutathione peroxidase,GSH-PX), Western blot. Results: MDA in vitamin C1 group was lower than that in poisoning group, and GSH-PX was higher than that in poisoning group. The liver and kidney function of vitamin C1 group was significantly improved than that of poisoning group (all P0.01). The MDA of vitamin C _ 2 group was higher than that of poisoning group, and the liver and kidney function of vitamin C _ 2 group was worse than that of poisoning group (P 0.01). The expression of Bcl-2 in the poisoning group was lower than that in the control group, the expression of Bcl-2 in the vitamin C1 group was higher than that in the poisoning group, and the expression of Bcl-2 in the vitamin C2 group was lower than that in the poisoning group (P0.01). However, there was no significant difference in lung function, wet-dry weight ratio and pathology between vitamin C _ 1 and C _ 2 groups (P 0.05). Conclusion: low dose vitamin C may have partial protective effect on liver and kidney function of PQ poisoned rats by anti-oxidation and inhibiting apoptosis induced by PQ, while high dose vitamin C can promote oxidation. At the same time, vitamin C did not show a protective effect on the lung after PQ poisoning.
【作者單位】： 重慶醫(yī)科大學(xué)第二臨床學(xué)院急救部;
【基金】：重慶市衛(wèi)生局基金(2013-2-036)~~
【分類號】：R595.4

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