RA患者外周血和關節(jié)炎大鼠破骨細胞miRNAs異常表達譜比較及特征性miRNA功能分析
發(fā)布時間：2019-02-16 20:00
【摘要】：目的:篩選類風濕關節(jié)炎(RA)患者外周血與關節(jié)炎大鼠破骨細胞的miRNAs異常表達譜,分析RA的特征性miRNAs及其功能。方法:采用miRNAs芯片,篩選RA患者與正常人外周血、共培養(yǎng)誘生的關節(jié)炎大鼠破骨細胞與正常基質細胞的miRNAs表達譜的差異;Real time PCR對芯片結果進行驗證;生物信息學方法對關鍵miRNA進行功能分析。結果:與正常人相比,RA患者外周血中具有差異表達的miRNAs有189個;與單核細胞比較,破骨細胞中具有差異表達的miRNAs有211個,其中miR-15b-5p等10個miRNAs在RA患者外周血及大鼠破骨細胞中均異常表達。Real time PCR驗證結果與芯片檢測結果具有一致性。生物信息學分析結果顯示特征性miRNA的靶基因顯著富集在VEGF,MAPK信號通路等信號通路。結論:部分miRNAs在RA患者外周血及關節(jié)炎大鼠破骨細胞中的異常表達具有一致性,其可能作為RA的特征性miRNAs,通過調控相關信號通路干預破骨細胞分化等病理過程。
[Abstract]:Aim: to screen the abnormal miRNAs expression profiles of osteoclasts in peripheral blood and osteoclasts of patients with rheumatoid arthritis (RA) and to analyze the characteristic miRNAs and its function of RA. Methods: the miRNAs expression profiles of osteoclasts and normal stromal cells in osteoclasts of induced arthritis rats were cocultured by miRNAs microarray, and the results were verified by; Real time PCR. Bioinformatics method was used to analyze the function of key miRNA. Results: there were 189 miRNAs in peripheral blood of RA patients. Compared with monocytes, there were 211 miRNAs differentially expressed in osteoclasts, of which 10 miRNAs, including miR-15b-5p, expressed. Real time PCR in peripheral blood of RA patients and in osteoclasts of rats. The results were consistent with the results of microarray detection. Bioinformatics analysis showed that the target genes of characteristic miRNA were significantly enriched in signal pathways such as VEGF,MAPK signaling pathway. Conclusion: the abnormal expression of partial miRNAs in peripheral blood of RA patients and osteoclasts in arthritis rats is consistent. It may be the characteristic miRNAs, of RA to interfere with osteoclast differentiation and other pathological processes by regulating related signaling pathways.
【作者單位】： 南京中醫(yī)藥大學;南京市中醫(yī)院;
【基金】：國家自然科學基金(81673937) 國家基金預研基金(No.14XYY01)資助
【分類號】：R593.22
本文編號：2424786
[Abstract]:Aim: to screen the abnormal miRNAs expression profiles of osteoclasts in peripheral blood and osteoclasts of patients with rheumatoid arthritis (RA) and to analyze the characteristic miRNAs and its function of RA. Methods: the miRNAs expression profiles of osteoclasts and normal stromal cells in osteoclasts of induced arthritis rats were cocultured by miRNAs microarray, and the results were verified by; Real time PCR. Bioinformatics method was used to analyze the function of key miRNA. Results: there were 189 miRNAs in peripheral blood of RA patients. Compared with monocytes, there were 211 miRNAs differentially expressed in osteoclasts, of which 10 miRNAs, including miR-15b-5p, expressed. Real time PCR in peripheral blood of RA patients and in osteoclasts of rats. The results were consistent with the results of microarray detection. Bioinformatics analysis showed that the target genes of characteristic miRNA were significantly enriched in signal pathways such as VEGF,MAPK signaling pathway. Conclusion: the abnormal expression of partial miRNAs in peripheral blood of RA patients and osteoclasts in arthritis rats is consistent. It may be the characteristic miRNAs, of RA to interfere with osteoclast differentiation and other pathological processes by regulating related signaling pathways.
【作者單位】： 南京中醫(yī)藥大學;南京市中醫(yī)院;
【基金】：國家自然科學基金(81673937) 國家基金預研基金(No.14XYY01)資助
【分類號】：R593.22
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