【摘要】：目的探討二甲雙胍(MET)對結(jié)腸癌干細(xì)胞增殖、凋亡及CD133表達(dá)的影響。方法免疫磁珠法從結(jié)腸癌細(xì)胞株SW480中分選出結(jié)腸癌干細(xì)胞,流式細(xì)胞儀檢測其表面標(biāo)志物CD133。不同濃度MET干預(yù)結(jié)腸癌干細(xì)胞后,CCK-8檢測24、48 h細(xì)胞增殖;流式細(xì)胞術(shù)檢測細(xì)胞凋亡;Western blot檢測CD133蛋白表達(dá)。結(jié)果不同濃度(1、5、10、20 mmol/L)MET干預(yù)后,24 h細(xì)胞活力[(89.3±16.5)%vs(61.6±16.8)%vs(45.1±14.7)%vs(35.4±13.7)%]與48 h細(xì)胞活力[(68.9±25.5)%vs(38.1±17.4)%vs(23.3±6.5)%vs(17.8±6.3)%]比較,差異有統(tǒng)計(jì)學(xué)意義(P0.01);與對照組比較,MET(20 mmol/L)干預(yù)后的細(xì)胞晚期凋亡率(13.5±3.3)%及總凋亡率(23.6±4.9)%差異有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01);MET(10、20 mmol/L)干預(yù)后CD133蛋白表達(dá)分別為(0.80±0.23)和(0.33±0.19),與對照組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 MET抑制結(jié)腸癌干細(xì)胞增殖、促進(jìn)其凋亡,減少其表面標(biāo)志物CD133蛋白表達(dá)。
[Abstract]:Objective to investigate the effects of metformin (MET) on the proliferation, apoptosis and CD133 expression of colon cancer stem cells. Methods Colon cancer stem cells were isolated from colon cancer cell line SW480 by immunomagnetic bead method. The surface marker CD133. was detected by flow cytometry. The proliferation of colon cancer stem cells was detected by CCK-8, and the expression of CD133 protein was detected by flow cytometry, apoptosis; Western blot was detected by flow cytometry. Results after the intervention of MET with different concentrations (1 / 10 ~ 10 ~ (20) mmol/L), 24 h cell viability [(89.3 鹵16.5)% vs (61.6 鹵16.8)% vs (45.1 鹵14.7)% vs (35.4 鹵13.7)%] and 48h cell viability [(68.9 鹵25.5)% vs (38.1 鹵17.4)% vs]. 23.3 鹵6.5% vs (17.8 鹵6.3%). The difference was statistically significant (P0.01). Compared with the control group, the late apoptosis rate (13.5 鹵3.3)% and the total apoptosis rate (23.6 鹵4.9)% after, MET (20 mmol/L intervention were significantly different (P0.05 or P0.01). The expression of CD133 protein was (0.80 鹵0.23) and (0.33 鹵0.19) after the intervention of MET (10 ~ 20 mmol/L), which was significantly different from that of the control group (P0.05). Conclusion MET inhibits the proliferation of colon cancer stem cells, promotes their apoptosis and reduces the expression of CD133 protein.
【作者單位】： 安徽醫(yī)科大學(xué)第一附屬醫(yī)院內(nèi)分泌科;
【基金】：安徽省自然科學(xué)基金(1508085MH150)
【分類號】：R587.1;R735.35

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