【摘要】：為探究蜂膠中4種黃酮成分(高良姜素、短葉松素、松屬素、柯因)對(duì)胰島素抵抗的改善作用。通過高濃度胰島素誘導(dǎo)的方式建立胰島素抵抗HepG2細(xì)胞模型;設(shè)立正常組、模型組、高良姜素處理組、短葉松素處理組、松屬素處理組、柯因處理組,測(cè)定各試驗(yàn)組對(duì)HepG2細(xì)胞增殖、葡萄糖消耗量、糖原含量、已糖激酶(hexokinase,HK)和丙酮酸激酶(pyruvate kinase,PK)的影響。結(jié)果顯示:4種黃酮成分在有效濃度范圍內(nèi)對(duì)胰島素抵抗HepG2細(xì)胞增殖均無顯著影響(P0.05);柯因、短葉松素作用效果不明顯(P0.05);高良姜素和松屬素均能不同程度地提高IR-HepG2細(xì)胞葡萄糖消耗量(glucose consumption,GC)、肝糖原含量、HK和PK活力(P0.05)。上述結(jié)果表明蜂膠中高良姜素和松屬素能較好地調(diào)節(jié)IR-HepG2糖代謝,改善胰島素抵抗,為蜂膠產(chǎn)品的深度開發(fā)提供參考。
[Abstract]:To investigate the effects of four flavonoids in propolis on insulin resistance. The HepG2 cell model of insulin resistance was established by high concentration insulin induction. HepG2 cell proliferation, glucose consumption, glycogen content and hexokinase, were measured in normal group, model group, ginger group, short leaf pine treatment group, pine treated group and Coin treatment group. HK and pyruvate kinase (pyruvate kinase,PK). The results showed that the four flavonoids had no significant effect on the proliferation of insulin-resistant HepG2 cells in the effective concentration range (P0.05), but the effects of Coin and short-leaf pine on the proliferation of insulin-resistant HepG2 cells were not significant (P0.05). Both gingerin and pine could increase glucose consumption (glucose consumption,GC), liver glycogen content, HK and competitive activity of IR-HepG2 cells in varying degrees (P0.05). The results indicated that gingerin and pine in propolis could regulate glucose metabolism of IR-HepG2 and improve insulin resistance, which could provide reference for the further development of propolis.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院蜜蜂研究所國家蜂產(chǎn)品加工技術(shù)研發(fā)中心;
【基金】：中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程項(xiàng)目(ASTIP)資助
【分類號(hào)】：R587.1;S896.6
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本文編號(hào)：2412055