【摘要】：目的研究不同劑量氯化汞對SD大鼠的血管內(nèi)皮損傷情況。方法將54只雄性SD大鼠簡單隨機(jī)分為四組,高、中、低氯化汞中毒組,每組15只,對照組9只;采用高(17 mg·kg~(-1)·d~(-1))、中(8.5 mg·kg~(-1)·d~(-1))、低劑量(4.25 mg·kg~(-1)·d~(-1))濃度氯化汞鹽水溶液灌胃建立SD大鼠亞急性汞中毒動物模型。中毒后7 d、14 d、21 d分別測定大鼠血細(xì)胞汞值、血清一氧化氮(NO)和內(nèi)皮素-1(ET-1)值,同時行循環(huán)內(nèi)皮細(xì)胞(CEC)計數(shù)。結(jié)果對照組血細(xì)胞汞值接近于零,中毒組與對照組差異均有統(tǒng)計學(xué)意義(P0.05)。血清ET-1組間比較,高劑量組與對照組差異有統(tǒng)計學(xué)意義(P0.05);組內(nèi)比較,不同時期ET-1值差異有統(tǒng)計學(xué)意義(P0.05)。血清NO組間比較,各劑量組與對照組差異均有統(tǒng)計學(xué)意義(P0.05);同期比較,不同中毒劑量組之間比較差異也有統(tǒng)計學(xué)意義(P0.05);組內(nèi)比較,不同時期之間差異均有統(tǒng)計學(xué)意義(P0.05)。血循環(huán)內(nèi)皮細(xì)胞計數(shù)組間比較,各劑量組與對照組差異均有統(tǒng)計學(xué)意義(P0.05);組內(nèi)比較,不同時期之間差異均有統(tǒng)計學(xué)意義(P0.05)。結(jié)論氯化汞可對血管內(nèi)皮細(xì)胞有直接損傷作用,且損傷作用與劑量、時間存在同一變化趨勢。
[Abstract]:Objective to study the vascular endothelial injury induced by different doses of mercuric chloride in SD rats. Methods 54 male SD rats were randomly divided into four groups: high, medium and low mercuric chloride poisoning group (n = 15) and control group (n = 9). In high (17 mg kg~ (-1) d ~ (-1),) (8.5 mg kg~ (-1) d ~ (-1),) Low dose (4.25 mg kg~ (-1) d ~ (-1) mercuric chloride solution was used to establish subacute mercury poisoning model of SD rats. The mercury levels in blood cells, serum nitric oxide (NO) and endothelin-1 (ET-1) levels were measured on the 7th day, 14th day and 21st day after poisoning. The circulating endothelial cells (CEC) were counted at the same time. Results the mercury level of blood cells in the control group was close to zero, and the difference between the poisoning group and the control group was statistically significant (P0.05). There were significant differences in serum ET-1 between high dose group and control group (P0.05); in intra-group comparison, there were significant differences in ET-1 values in different periods (P0.05). There were significant differences in serum NO between each dose group and the control group (P0.05), and there were significant differences between the different toxic dose groups (P0.05) in the same period. Comparison within the group, the differences between different periods were statistically significant (P0.05). Blood circulation endothelial cell count between the group, each dose group and the control group were statistically significant difference (P0.05); intra-group comparison, the differences between different periods were statistically significant (P0.05). Conclusion Mercury chloride can directly injure vascular endothelial cells, and the damage has the same trend as dose and time.
【作者單位】： 軍事醫(yī)學(xué)科學(xué)院附屬醫(yī)院神經(jīng)內(nèi)科;
【基金】：國家科技支撐計劃課題(編號:2012BAI38B01)
【分類號】：R114

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