【摘要】：目的:探討藍(lán)斑核(LC)orexin-A對(duì)肥胖抵抗(OR)大鼠自發(fā)活動(dòng)的影響及機(jī)制并進(jìn)一步探究LC的orexin調(diào)控是否與肥胖抵抗相關(guān)。方法:雄性SD大鼠和選擇性培育的OR大鼠,一側(cè)LC置管,緩慢均勻注射0.5μL orexin-A或人工合成腦脊液,采用大鼠自發(fā)活動(dòng)檢測(cè)裝置SPA紅外線活動(dòng)傳感箱,測(cè)定大鼠自發(fā)活動(dòng)量(SPA),間接測(cè)熱法測(cè)定其能量消耗,定量磁共振身體組成分析器檢測(cè)大鼠脂肪重和肌肉重。大鼠肥胖程度用體脂百分比表示。結(jié)果:與SD大鼠相比,OR大鼠的總體重、脂體重和去脂體重均顯著降低(P0.05)。OR大鼠肥胖程度明顯小于SD大鼠(P0.05)。OR大鼠在3月齡時(shí)主要表現(xiàn)為水平活動(dòng),6月齡時(shí)垂直活動(dòng)增加,提示OR大鼠隨年齡增長(zhǎng)活動(dòng)量顯著增多(P0.05)。與SD大鼠相比,OR大鼠SPA水平顯著增高(P0.05),非靜息能量消耗顯著增多(P0.05),但日間能量消耗無(wú)明顯差異(P0.05)。提示,OR大鼠夜間活動(dòng)增多可導(dǎo)致能量消耗增加,體重下降。LC注射orexin-A能明顯增加OR大鼠的SPA(P0.05),并呈現(xiàn)劑量依賴性,但對(duì)大鼠的攝食量無(wú)明顯影響(P0.05)。該結(jié)果提示,LC的orexin-A系統(tǒng)在OR大鼠肥胖抵抗中起著重要作用,此作用可能是通過(guò)其對(duì)SPA的調(diào)控實(shí)現(xiàn)的。結(jié)論:藍(lán)斑核orexin-A對(duì)肥胖大鼠自發(fā)活動(dòng)有重要影響,該作用與肥胖抵抗相關(guān)。
[Abstract]:Aim: to investigate the effect and mechanism of locus coeruleus (LC) orexin-A on spontaneous activity in obese resistant (OR) rats and to explore whether the orexin regulation of LC is related to obesity resistance. Methods: male SD rats and selectively cultured OR rats were injected with 0.5 渭 L orexin-A or synthetic cerebrospinal fluid (CSF) slowly and evenly. SPA infrared moving-sensing box was used to detect the spontaneous activity of rats. The energy consumption was measured by (SPA), indirect calorimetry and the fat weight and muscle weight were measured by quantitative magnetic resonance body composition analyzer. The degree of obesity in rats was expressed as a percentage of body fat. Results: compared with SD rats, the total weight, fat weight and degreasing weight of OR rats were significantly decreased (P0.05). OR rats were significantly less obese than SD rats (P0.05). OR rats at 3 months of age) showed horizontal activity. The vertical activity increased at 6 months, suggesting that the activity of OR rats increased significantly with age (P0.05). Compared with SD rats, SPA level of OR rats was significantly higher (P0.05), non-resting energy expenditure significantly increased (P0.05), but there was no significant difference between day energy expenditure (P0.05). The results suggested that increased night-time activity in OR rats resulted in increased energy consumption and weight loss. LC injection of orexin-A could significantly increase SPA of OR rats (P0.05) in a dose-dependent manner, but had no significant effect on the feeding intake of OR rats (P0.05). These results suggest that the orexin-A system of LC plays an important role in obesity resistance in OR rats, and this effect may be achieved through its regulation of SPA. Conclusion: orexin-A plays an important role in the spontaneous activity of obese rats, which is related to obesity resistance.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R589.2

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