【摘要】：目的：探討TCF7L2基因多態(tài)性位點rs13266634與新疆省漢族人群2型糖尿病之間的相關性。方法：將研究對象分為糖尿病組(n=116)和對照組(n=126)兩組,研究對象均來自于新疆省漢族人群。通過聚合酶鏈式反應-限制性內切酶片段長度多態(tài)性(PCR-RFLP)技術對研究人群(126名健康人的對照組和116名2型糖尿病患者的糖尿病組)的TCF7L2基因rs13266634多態(tài)性進行檢測,并比較基因型和等位基因頻率。結果：糖尿病組和正常對照組年齡、體重指數(shù)、甘油三酯、低密度脂蛋白及高密度脂蛋白之間無顯著差異性,P均大于0.05；而糖化血紅蛋白、收縮壓、舒張壓、空腹血糖、餐后2小時血糖及總膽固醇之間有顯著差異,均P0.05。TCF7L2基因多態(tài)性位點rs13266634的基因型在糖尿病組和正常對照組中的分布平率分別為：按TT、CT、CC順序為20.7%、48.6%、30.6%和28.6%、42.1%、29.4%,兩組間差異均無統(tǒng)計學意義(P0.05); TCF7L2基因rs290487多態(tài)位點在兩組間的基因型分布符合Hardy-Weinberg平衡定律。Logistic回歸校正了年齡、體重指數(shù)、性別等因素后,TCF7L2基因rs13266634多態(tài)性與2型糖尿病的發(fā)生無相關性(P0.05)。結論：新疆漢族人群TCF7L2基因rs13266634 T/C多態(tài)性可能與2型糖尿病的發(fā)生無相關性。
[Abstract]:Objective: to investigate the association between TCF7L2 gene polymorphism rs13266634 and type 2 diabetes mellitus in Han nationality of Xinjiang province. Methods: the subjects were divided into two groups: diabetes mellitus group (n-116) and control group (n-126). All the subjects were from Han nationality in Xinjiang province. Polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) was used to detect the rs13266634 polymorphism of TCF7L2 gene in 126 healthy controls and 116 diabetic patients with type 2 diabetes. Genotypes and alleles were compared. Results: there was no significant difference in age, body mass index, triglyceride, low density lipoprotein and high density lipoprotein between diabetic group and normal control group (P > 0.05). There were significant differences in glycosylated hemoglobin, systolic blood pressure, diastolic blood pressure, fasting blood glucose, 2 hours postprandial blood glucose and total cholesterol. The distribution rates of rs13266634 genotypes in the diabetic group and the normal control group were as follows: according to the order of TT,CT,CC, rs13266634 was 20.78.60.30.6% and 28.6%, respectively, and the distribution rate of rs13266634 was 42.1% in the diabetic group and in the normal control group, respectively. 29.4 there was no significant difference between the two groups (P0.05); The genotype distribution of rs290487 polymorphism of TCF7L2 gene in the two groups was in accordance with the Hardy-Weinberg equilibrium law. Logistic regression adjusted for age, body mass index, sex and other factors, TCF7L2 gene rs13266634 polymorphism was not associated with the occurrence of type 2 diabetes (P0.05). Conclusion: TCF7L2 gene rs13266634 T / C polymorphism may not be associated with type 2 diabetes in Xinjiang Han population.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R587.1
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本文編號：2384768