【摘要】：主要利用二維相關(guān)光譜分析方法考察了血液中主要成分、測(cè)量過(guò)程中的系統(tǒng)漂移等因素對(duì)近紅外光譜中的葡萄糖分子特異性的影響。首先測(cè)量了葡萄糖水溶液的近紅外透射光譜,以葡萄糖濃度為外擾,計(jì)算二維相關(guān)同步譜及異步譜,通過(guò)對(duì)比分析確定了葡萄糖在合頻和倍頻區(qū)域的特征吸收峰位置。當(dāng)葡萄糖水溶液中加入少量白蛋白后,其二維相關(guān)譜中葡萄糖的一級(jí)倍頻吸收峰(1 590nm)和二級(jí)倍頻吸收峰(1 195nm)出現(xiàn)了不同源的現(xiàn)象,表明白蛋白可能破壞了葡萄糖的特異性;進(jìn)一步開(kāi)展了人體口服葡萄糖耐量實(shí)驗(yàn),測(cè)量了手掌部位的漫反射光譜,其二維相關(guān)譜中葡萄糖的同源性吸收也遭到破壞。另外,由于葡萄糖濃度變化引起的光譜變異信息較小,很容易被系統(tǒng)漂移掩蓋,一般需要進(jìn)行背景修正。在葡萄糖水溶液的透射實(shí)驗(yàn)和人體漫反射實(shí)驗(yàn)中,分別引入純水樣本和5%標(biāo)準(zhǔn)漫反射板作為參考,以時(shí)間為擾動(dòng)的二維相關(guān)光譜分析的結(jié)果表明,經(jīng)過(guò)參考樣本的背景校正后,系統(tǒng)漂移在二維同步切線譜中引起的譜峰偏移變小,葡萄糖的特征吸收被增強(qiáng)。因此,在近紅外光譜無(wú)創(chuàng)血糖檢測(cè)中,應(yīng)盡量避開(kāi)與葡萄糖特征吸收不同源的波段,并有必要采取一定的參考測(cè)量方法以提高檢測(cè)的特異性。
[Abstract]:The effects of the main components in the blood and the systematic drift during the measurement on the molecular specificity of glucose in the near infrared spectroscopy were investigated by two-dimensional correlation spectroscopy. The near infrared transmission spectra of glucose aqueous solution were measured at first. Using glucose concentration as an external disturbance, the two dimensional correlation synchronous spectra and asynchronous spectra were calculated, and the characteristic absorption peaks of glucose in the frequency-doubling and frequency-doubling regions were determined by comparative analysis. When a small amount of albumin was added to glucose aqueous solution, the first-order frequency doubling absorption peak (1 590nm) and the second order frequency doubling absorption peak (1 195nm) of glucose in the two-dimensional correlation spectrum showed different homology. It was suggested that albumin might destroy the specificity of glucose. The oral glucose tolerance test was carried out and the diffuse reflectance spectra of the palm were measured. The homologous absorption of glucose in the two-dimensional correlation spectrum was also destroyed. In addition, the spectral variation information caused by glucose concentration change is small, which is easy to be masked by system drift, so background correction is usually needed. In the transmission experiment of glucose aqueous solution and the human diffuse reflection experiment, the pure water sample and the 5% standard diffuse reflectance plate were introduced as reference respectively. After background correction of the reference sample, the peak offset caused by the system drift in the 2-D synchronous tangent spectrum is reduced, and the characteristic absorption of glucose is enhanced. Therefore, in NIR non-invasive blood glucose detection, we should avoid the band which is not homologous to the characteristic absorption of glucose, and it is necessary to adopt certain reference measurement methods to improve the specificity of the detection.
【作者單位】： 天津大學(xué)精密測(cè)試技術(shù)及儀器國(guó)家重點(diǎn)實(shí)驗(yàn)室;天津大學(xué)精密儀器與光電子工程學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(60938002) 國(guó)家(863)計(jì)劃項(xiàng)目(2012AA022602)資助
【分類(lèi)號(hào)】：R587.1;O657.3

【相似文獻(xiàn)】

相關(guān)期刊論文 前6條

1 周群;;國(guó)際會(huì)議消息[J];光譜學(xué)與光譜分析;2013年08期

2 周群;;國(guó)際會(huì)議消息[J];光譜學(xué)與光譜分析;2013年06期

3 宋海燕;程旭;;水分對(duì)土壤近紅外光譜檢測(cè)影響的二維相關(guān)光譜解析[J];光譜學(xué)與光譜分析;2014年05期

4 王夢(mèng)吟;武培怡;;移動(dòng)窗口二維相關(guān)光譜技術(shù)[J];化學(xué)進(jìn)展;2010年05期

5 張瑤;鄭立華;孫紅;李民贊;;基于二維相關(guān)光譜的水體葉綠素含量探測(cè)[J];光譜學(xué)與光譜分析;2014年02期

6 ;[J];;年期

相關(guān)會(huì)議論文 前4條

1 周群;孫素琴;詹達(dá)琦;尉志武;;移動(dòng)窗口二維相關(guān)光譜法的方法學(xué)研究[A];第十五屆全國(guó)分子光譜學(xué)術(shù)報(bào)告會(huì)論文集[C];2008年

2 張卓勇;王鳳霞;范國(guó)強(qiáng);Peter de B.Harrington;;中紅外-近紅外二維相關(guān)光譜法用于大黃的分類(lèi)鑒別[A];第十五屆全國(guó)分子光譜學(xué)術(shù)報(bào)告會(huì)論文集[C];2008年

3 張曉星;王震;胡耀垓;任江波;唐炬;;基于二維相關(guān)光譜的SF_6分解組分紅外檢測(cè)識(shí)別[A];2010電工測(cè)試技術(shù)學(xué)術(shù)交流會(huì)論文集[C];2010年

4 吳玉清;;正交信號(hào)矯正法在消除非變量誘導(dǎo)信號(hào)對(duì)二維相關(guān)光譜干擾中的應(yīng)用[A];第十四屆全國(guó)分子光譜學(xué)術(shù)會(huì)議論文集[C];2006年

相關(guān)碩士學(xué)位論文 前4條

1 覃方麗;二維相關(guān)光譜研究及應(yīng)用[D];中國(guó)農(nóng)業(yè)大學(xué);2003年

2 劉婷;二維相關(guān)光譜法在藥物分析復(fù)雜體系中的應(yīng)用[D];北京協(xié)和醫(yī)學(xué)院;2010年

3 王夢(mèng)吟;二維相關(guān)光譜性質(zhì)探索及其在復(fù)雜變化體系中的應(yīng)用[D];復(fù)旦大學(xué);2011年

4 修子玉;含有聯(lián)酰胺基元液晶分子的合成與二維相關(guān)光譜研究[D];吉林大學(xué);2009年

，

本文編號(hào)：2339995