【摘要】：比較兩種高脂乳脂配方復(fù)制高脂血癥模型大鼠的血脂、肝重及肝病理的變化,為高血脂癥的預(yù)防、治療以及研究選擇合適的動(dòng)物造模方法。比較兩次試驗(yàn)中,正常對(duì)照1組和高脂高糖乳劑1組、正常對(duì)照2組和高脂高糖乳劑2組SD大鼠,10只/組,按10mL/kg分別給予相應(yīng)的高脂高糖乳劑連續(xù)造模40d,正常對(duì)照組則給予等量純凈水,分別于造模前、造模10d、造模40d時(shí)測(cè)定大鼠體重、血清總膽固醇(TC)、甘油三酯(TG)、高密度脂蛋白膽固醇(HDLC)、低密度脂蛋白膽固醇(LDLC)含量及HDLC/TC比值,并測(cè)定肝臟重量以及肝臟病理組織學(xué)的變化。結(jié)果顯示,與相應(yīng)的正常對(duì)照組比較,第一次試驗(yàn)中的高脂高糖乳脂1配方造模40d,可使血脂含量升高,肝臟細(xì)胞脂肪變性,第二次試驗(yàn)中的高脂高糖乳脂2配方造模40d,只能使血脂含量升高,未見肝細(xì)胞脂肪變性。與高脂高糖乳脂1組相比,高脂高糖乳脂2組造模40d體重下降幅度較小(P0.05),TC、TG、LDLC含量升高幅度較少(P0.05),HDLC/TC比值降低幅度較少(P0.05),肝臟重量增大幅度較大(P0.05),肝臟系數(shù)增大幅度較少(P0.05),肝臟未產(chǎn)生肝細(xì)胞脂肪變性。結(jié)果表明,第一次試驗(yàn)中的高脂高糖乳劑1配方可以制作混合型高脂血癥伴脂肪肝大鼠模型,第二次試驗(yàn)中的高脂高糖乳脂2配方可以制作混合型高脂血癥大鼠模型。
[Abstract]:To compare the changes of serum lipids, liver weight and liver pathology in rats with hyperlipidemia induced by two kinds of formula of hyperlipidemia, to choose suitable animal models for prevention, treatment and study of hyperlipidemia. In the two experiments, 10 SD rats in normal control group, high fat and high sugar emulsion group 1, normal control group 2 and high fat and high sugar emulsion group, 10 rats in each group were given corresponding high fat and high sugar emulsion for 40 days, respectively. The normal control group was given the same amount of pure water. The rats' body weight and serum total cholesterol (TC),) triglyceride (TG),) high density lipoprotein cholesterol (HDLC),) were measured after 10 days and 40 days before modeling. Low density lipoprotein cholesterol (LDL-C) (LDLC) content and HDLC/TC ratio, liver weight and liver histopathology were measured. The results showed that, compared with the normal control group, the high fat and high sugar formula 1 in the first trial made the model for 40 days, which resulted in the increase of blood lipid content, the fatty degeneration of liver cells, and the high fat and high sugar formula in the second test for 40 days. Can only make blood fat content increase, did not see liver cell adipose degeneration. Compared with high fat and high sugar milk fat group 1, the weight loss of model group 2 was smaller (P0.05), the increase of TC,TG,LDLC content was less (P0.05), and the ratio of HDLC/TC decreased less (P0.05). Liver weight increased significantly (P0.05), liver coefficient increased less (P0.05), liver did not produce hepatocyte fatty degeneration. The results showed that the formula of hyperlipidemia and hyperglycemia emulsion 1 in the first experiment could make the rat model of mixed hyperlipidemia with fatty liver, and the formula of high fat and high sugar emulsion 2 in the second test could make the rat model of mixed hyperlipidemia.
【作者單位】： 廣東省醫(yī)學(xué)實(shí)驗(yàn)動(dòng)物中心;
【基金】：廣東省醫(yī)學(xué)科研基金項(xiàng)目(B2014060) 廣東省科技計(jì)劃項(xiàng)目(2016A030303026)
【分類號(hào)】：R-332;R589.2

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