Toll樣受體4與IL-7和IL-10在干燥綜合征患者唇腺組織中表達(dá)及其意義研究
發(fā)布時(shí)間：2018-11-14 16:58
【摘要】：目的:了解干燥綜合征患者唇腺組織內(nèi)TLR4與IL-7和IL-10的表達(dá)情況,并分析各因子與其它實(shí)驗(yàn)室指標(biāo)之間的相關(guān)性,探索TLR4及其相關(guān)細(xì)胞因子在干燥綜合征發(fā)生、發(fā)展過(guò)程中的作用,為干燥綜合征的臨床診斷與治療提供新思路。方法:.以蚌埠醫(yī)學(xué)院第一附屬醫(yī)院于2010年2月至2013年10月間風(fēng)濕免疫科收治的74例干燥綜合征患者為研究組,以同期收治的32例非干燥綜合征患者為對(duì)照組,獲取所有患者的唇腺病理切片采用Elivision免疫組化染色方法檢測(cè)、同時(shí)采用人工計(jì)數(shù)方法進(jìn)行染色結(jié)果的定性和半定量分析,進(jìn)行如下研究:(1)分析研究組和對(duì)照組患者唇腺內(nèi)的TLR4、IL-7、IL-10表達(dá)陽(yáng)性率、陽(yáng)性細(xì)胞染色積分和顯色強(qiáng)度。(2)分析TLR4、IL-7、IL-10三種因子陽(yáng)性表達(dá)之間的相關(guān)性,探討TLR4、IL-7、IL-10與其它實(shí)驗(yàn)室指標(biāo)之間的相關(guān)性。(3)對(duì)比原發(fā)性和繼發(fā)性干燥綜合征患者及對(duì)照組唇腺內(nèi)TLR4、IL-7、IL-10的陽(yáng)性表達(dá)情況有無(wú)差異,并探討其意義。最后借助于SPSS13.0軟件包進(jìn)行統(tǒng)計(jì)學(xué)分析。計(jì)量數(shù)據(jù)以(±s)表示,組間對(duì)比行t檢驗(yàn)。計(jì)數(shù)據(jù)以n(%)表示,組間對(duì)比行x2檢查。多組間對(duì)比行AVONA中的SNK-q檢驗(yàn)。相關(guān)性分析采用Spearman相關(guān)分析。組間檢驗(yàn)水平a=0.05,依據(jù)組數(shù)進(jìn)行修正。以P0.05表示差異具有統(tǒng)計(jì)學(xué)意義,以P0.01表示組間差異具有顯著統(tǒng)計(jì)學(xué)意義。結(jié)果:(1)TLR4在研究組患者唇腺內(nèi)的陽(yáng)性表達(dá)率為79.72%,對(duì)照組為53.12%,差異具有統(tǒng)計(jì)學(xué)意義(x2=5.471,P=0.031)。研究組患者的TLR4陽(yáng)性細(xì)胞顯色程度在實(shí)驗(yàn)組為1.27±0.29,在對(duì)照組中為0.46±0.51,P=0.013;研究組TLR4陽(yáng)性細(xì)胞染色積分在為3.24±3.07,在對(duì)照組為0.58±0.66,P0.001且差異具有統(tǒng)計(jì)學(xué)意義。(2)研究組患者唇腺內(nèi)IL-7的陽(yáng)性表達(dá)率為85.14%,對(duì)照組為21.87%,差異具有統(tǒng)計(jì)學(xué)意義(x2=17.539,P=0.000)。研究組IL-7陽(yáng)性細(xì)胞顯色程度為1.31±0.27,在對(duì)照組中為0.26±0.11,P=0.021;研究組IL-7陽(yáng)性細(xì)胞染色積分為3.37±1.61,在對(duì)照組為0.54±0.67,P=0.014;且差異具有統(tǒng)計(jì)學(xué)意義。(3)研究組患者唇腺內(nèi)IL-10的陽(yáng)性表達(dá)率為82.43%,對(duì)照組為34.28%,兩組間差異具有統(tǒng)計(jì)學(xué)意義(x2=10.204,P=0.013)。研究組IL-10的陽(yáng)性細(xì)胞顯色程度為1.49±0.22,在對(duì)照組為0.31±0.17,P=0.019,研究組IL-10陽(yáng)性細(xì)胞染色積分為3.41±1.04,在對(duì)照組為0.59±0.61,P=0.024,且差異具有統(tǒng)計(jì)學(xué)意義。(4)TLR4、IL-7、IL-10三種因子的陽(yáng)性表達(dá)率間均相互呈正相關(guān),且具有統(tǒng)計(jì)學(xué)意義(P0.05)。(5)原發(fā)性干燥綜合征與繼發(fā)性干燥綜合征患者唇腺內(nèi)的TLR4、IL-7、IL-10陽(yáng)性表達(dá)率均明顯高于對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。但原發(fā)性和繼發(fā)性干燥綜合征患者腺體內(nèi)的TLR4、IL-7、IL-10陽(yáng)性表達(dá)情況無(wú)統(tǒng)計(jì)學(xué)差異(P0.05)。(6)IL-7和IL-10的陽(yáng)性表達(dá)與ESR呈正相關(guān)(r=0.539,P=0.003;r=0.494,P=0.013)。TLR4的陽(yáng)性表達(dá)則分別與Ig M(r=0.358,P=0.03),Ig A(r=0.461,P=0.012)、ESR(r=0.303,P=0.032)等免疫指標(biāo)或炎性指標(biāo)呈正相關(guān),差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:(1)干燥綜合征患者唇腺內(nèi)的TLR4與IL-7和IL-10的表達(dá)水平均明顯高于正常對(duì)照組,但原發(fā)性和繼發(fā)性患者之間無(wú)顯著差異,提示TLR4信號(hào)轉(zhuǎn)導(dǎo)途徑及其相關(guān)細(xì)胞因子可能參與干燥綜合征發(fā)病過(guò)程;(2)TLR4與IL7、IL10的表達(dá)水平與ESR及血清Ig M、Ig A的表達(dá)呈正相關(guān),提示TLR4與IL7、IL10的表達(dá)水平與病情活動(dòng)有一定的相關(guān)性,可作為臨床判斷病情活動(dòng)度的參考指標(biāo);(3)TLR4與IL-7、IL-10的表達(dá)與多種炎性或免疫因子可能有一定的相關(guān)性,針對(duì)TLR4信號(hào)導(dǎo)途徑的靶向治療可能有利于控制干燥綜合征病情的發(fā)展。
[Abstract]:Objective: To study the expression of TLR4 and IL-7 and IL-10 in the lip gland of patients with dry syndrome, and to analyze the correlation between the factors and other laboratory indexes, and to explore the role of TLR4 and its associated cytokines in the pathogenesis and development of dry syndrome. and provides a new thought for clinical diagnosis and treatment of the dry syndrome. Method:. In the first Affiliated Hospital of Bengbu Medical College from February 2010 to October 2013, 74 patients with dry syndrome were treated as the study group, and 32 patients with non-dry syndrome treated in the same period were the control group. The positive rate of TLR4, IL-7 and IL-10 in the labial gland of the study group and the control group was analyzed. The staining and developing intensity of positive cells. (2) The correlation between the three factors of TLR4, IL-7 and IL-10 was analyzed, and the correlation between TLR4, IL-7, IL-10 and other laboratory indexes was discussed. (3) The positive expression of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome and the control group were different, and their significance was discussed. Finally, the statistical analysis was performed with the help of the SPSS13.0 software package. The measurement data is expressed as (% s), and the inter-group comparison line t is tested. The count data is represented by n (%), and the inter-group comparison line x2 is checked. The SNK-q test in a multi-group comparison line, AVONA. The correlation analysis was analyzed by Spearman. The level of test between the groups was a = 0.05, and the group number was corrected. The difference between the groups was statistically significant with the difference of P0.05, and the difference between the groups was statistically significant. Results: (1) The positive expression rate of TLR4 in the labial gland of the study group was 72.72%, the control group was 53.12%, and the difference was of statistical significance (x2 = 5.471, P = 0.031). The color of TLR4 positive cells in the study group was 1.27 to 0.29 in the experimental group and 0.46 to 0.51, P = 0.013 in the control group. The staining and integration of TLR4 positive cells in the study group were 3.24 and 3.07, and 0.58, 0.66, P0.001 in the control group, and the difference was of statistical significance. (2) The positive expression of IL-7 in the lip glands of the study group was 85. 14%, the control group was 21. 87%, and the difference was statistically significant (x2 = 175.539, P = 0.000). The level of IL-7 positive cells in the study group was 1.31 and 0.27. In the control group, the staining score of IL-7 positive cells was 0.26, 0.11, P = 0.021, and that of IL-7 positive cells in the study group was 3.37-1.61, and the control group was 0.54-0.67, P = 0.014; and the difference was of statistical significance. (3) The positive expression rate of IL-10 in the labial gland of the study group was 82.43%, the control group was 34. 28%, and the difference between the two groups was statistically significant (x2 = 10.204, P = 0.013). The staining degree of IL-10 positive cells in the study group was 1.49 and 0.22. In the control group, the staining score of IL-10 positive cells was 3.41-1.04, the control group was 0.59-0.61, P = 0.024, and the difference was of statistical significance. (4) The positive expression rates of TLR4, IL-7 and IL-10 were positively correlated with each other and were of statistical significance (P0.05). (5) The positive rate of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome was significantly higher than that in the control group (P0.05). The expression of TLR4, IL-7 and IL-10 in the gland of patients with primary and secondary drying syndrome was not statistically different (P0.05). (6) The positive expression of IL-7 and IL-10 was positively correlated with ESR (r = 0.539, P = 0.003; r = 0.494, P = 0.013). The positive expression of TLR4 was positively correlated with that of Ig M (r = 0.358, P = 0.03), Ig A (r = 0.461, P = 0.012), and ESR (r = 0.303, P = 0.032). Conclusion: (1) The expression level of TLR4 and IL-7 and IL-10 in the lip gland of the patients with dry syndrome is significantly higher than that in the normal control group, but there is no significant difference between the primary and secondary patients, suggesting that the TLR4 signal transduction pathway and its associated cytokines may be involved in the pathogenesis of the dry syndrome; (2) The expression level of TLR4 and IL7 and IL10 is positively correlated with the expression of the ESR and the serum Ig M and Ig A, suggesting that the expression level of TLR4 and IL7 and IL10 has a certain correlation with the activity of the disease, and can be used as a reference index for clinical judgment of the disease activity; (3) TLR4 and IL-7, The expression of IL-10 may be related to various inflammatory or immune factors, and the targeted therapy for TLR4 signaling pathway may be beneficial to the control of the development of dry syndrome.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R593.2
本文編號(hào)：2331763
[Abstract]:Objective: To study the expression of TLR4 and IL-7 and IL-10 in the lip gland of patients with dry syndrome, and to analyze the correlation between the factors and other laboratory indexes, and to explore the role of TLR4 and its associated cytokines in the pathogenesis and development of dry syndrome. and provides a new thought for clinical diagnosis and treatment of the dry syndrome. Method:. In the first Affiliated Hospital of Bengbu Medical College from February 2010 to October 2013, 74 patients with dry syndrome were treated as the study group, and 32 patients with non-dry syndrome treated in the same period were the control group. The positive rate of TLR4, IL-7 and IL-10 in the labial gland of the study group and the control group was analyzed. The staining and developing intensity of positive cells. (2) The correlation between the three factors of TLR4, IL-7 and IL-10 was analyzed, and the correlation between TLR4, IL-7, IL-10 and other laboratory indexes was discussed. (3) The positive expression of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome and the control group were different, and their significance was discussed. Finally, the statistical analysis was performed with the help of the SPSS13.0 software package. The measurement data is expressed as (% s), and the inter-group comparison line t is tested. The count data is represented by n (%), and the inter-group comparison line x2 is checked. The SNK-q test in a multi-group comparison line, AVONA. The correlation analysis was analyzed by Spearman. The level of test between the groups was a = 0.05, and the group number was corrected. The difference between the groups was statistically significant with the difference of P0.05, and the difference between the groups was statistically significant. Results: (1) The positive expression rate of TLR4 in the labial gland of the study group was 72.72%, the control group was 53.12%, and the difference was of statistical significance (x2 = 5.471, P = 0.031). The color of TLR4 positive cells in the study group was 1.27 to 0.29 in the experimental group and 0.46 to 0.51, P = 0.013 in the control group. The staining and integration of TLR4 positive cells in the study group were 3.24 and 3.07, and 0.58, 0.66, P0.001 in the control group, and the difference was of statistical significance. (2) The positive expression of IL-7 in the lip glands of the study group was 85. 14%, the control group was 21. 87%, and the difference was statistically significant (x2 = 175.539, P = 0.000). The level of IL-7 positive cells in the study group was 1.31 and 0.27. In the control group, the staining score of IL-7 positive cells was 0.26, 0.11, P = 0.021, and that of IL-7 positive cells in the study group was 3.37-1.61, and the control group was 0.54-0.67, P = 0.014; and the difference was of statistical significance. (3) The positive expression rate of IL-10 in the labial gland of the study group was 82.43%, the control group was 34. 28%, and the difference between the two groups was statistically significant (x2 = 10.204, P = 0.013). The staining degree of IL-10 positive cells in the study group was 1.49 and 0.22. In the control group, the staining score of IL-10 positive cells was 3.41-1.04, the control group was 0.59-0.61, P = 0.024, and the difference was of statistical significance. (4) The positive expression rates of TLR4, IL-7 and IL-10 were positively correlated with each other and were of statistical significance (P0.05). (5) The positive rate of TLR4, IL-7 and IL-10 in the lip glands of patients with primary and secondary drying syndrome was significantly higher than that in the control group (P0.05). The expression of TLR4, IL-7 and IL-10 in the gland of patients with primary and secondary drying syndrome was not statistically different (P0.05). (6) The positive expression of IL-7 and IL-10 was positively correlated with ESR (r = 0.539, P = 0.003; r = 0.494, P = 0.013). The positive expression of TLR4 was positively correlated with that of Ig M (r = 0.358, P = 0.03), Ig A (r = 0.461, P = 0.012), and ESR (r = 0.303, P = 0.032). Conclusion: (1) The expression level of TLR4 and IL-7 and IL-10 in the lip gland of the patients with dry syndrome is significantly higher than that in the normal control group, but there is no significant difference between the primary and secondary patients, suggesting that the TLR4 signal transduction pathway and its associated cytokines may be involved in the pathogenesis of the dry syndrome; (2) The expression level of TLR4 and IL7 and IL10 is positively correlated with the expression of the ESR and the serum Ig M and Ig A, suggesting that the expression level of TLR4 and IL7 and IL10 has a certain correlation with the activity of the disease, and can be used as a reference index for clinical judgment of the disease activity; (3) TLR4 and IL-7, The expression of IL-10 may be related to various inflammatory or immune factors, and the targeted therapy for TLR4 signaling pathway may be beneficial to the control of the development of dry syndrome.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R593.2
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