免疫缺陷小鼠體內(nèi)人源化骨髓微環(huán)境的重建
發(fā)布時(shí)間：2018-11-11 12:57
【摘要】：目的:通過在免疫缺陷小鼠體內(nèi)重建人源的骨髓微環(huán)境,為進(jìn)一步研究人異常的骨髓微環(huán)境在人白血病發(fā)生發(fā)展中的作用提供模型。方法:通過反復(fù)凍融濃縮后血小板獲得人血小板裂解液(HPL),以α-MEM作為基礎(chǔ)培養(yǎng)液分別添加10%HPL和10%胎牛血清(FBS)培養(yǎng)骨髓來源的間充質(zhì)干細(xì)胞。比較兩種添加成分對(duì)MSC形態(tài)、免疫表型、多向分化能力以及增殖能力的影響;將HPL培養(yǎng)的MSC接種于β-TCP支架材料并移植到免疫缺陷小鼠背部皮下,8-12周后取出移植體做HE染色,觀察MSC在體內(nèi)形成骨以及骨髓結(jié)構(gòu)的能力。結(jié)果:用HPL和FBS培養(yǎng)的M SC均呈現(xiàn)長梭形纖維樣細(xì)胞結(jié)構(gòu),均具有多向分化能力;兩種體系培養(yǎng)的M SC免疫表型無明顯區(qū)別,但用人血小板裂解液培養(yǎng)的MSC具有更強(qiáng)的增殖能力和向成骨分化能力;用人血小板裂解液培養(yǎng)的MSC具有在體內(nèi)形成骨組織樣結(jié)構(gòu)的能力。結(jié)論:HPL培養(yǎng)的MSC具有更強(qiáng)的增殖能力以及成骨分化潛能,它能在NOD/SCID鼠上重建人源化骨髓微環(huán)境。
[Abstract]:Aim: to provide a model for the further study of the role of human bone marrow microenvironment in the development of human leukemia by reconstructing the human bone marrow microenvironment in immunodeficient mice. Methods: human platelet lysate (HPL), supplemented with 10%HPL and 10% fetal bovine serum (FBS) supplemented with 偽-MEM was used to culture mesenchymal stem cells derived from bone marrow by repeatedly freezing and thawing concentrated platelets. The effects of two additive components on the morphology, immunophenotype, multidirectional differentiation and proliferation of MSC were compared. The MSC cultured by HPL was inoculated into 尾-TCP scaffold and transplanted into the subcutaneous of the back of immunodeficient mice. After 8-12 weeks, the grafts were removed for HE staining to observe the ability of MSC to form bone and bone marrow structure in vivo. Results: M SC cultured with HPL and FBS showed long fusiform fibrous cell structure and multidirectional differentiation ability. There was no significant difference in the immunophenotype of M SC between the two systems, but the MSC cultured in human platelet lysate had stronger proliferative and osteogenic ability. MSC cultured with human platelet lysate has the ability to form bone-like structure in vivo. Conclusion: MSC cultured with HPL has stronger proliferative and osteogenic potential, and it can reconstruct human bone marrow microenvironment in NOD/SCID mice.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院血液學(xué)研究所血液病醫(yī)院實(shí)驗(yàn)血液學(xué)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81370599)
【分類號(hào)】：R593.3
本文編號(hào)：2324905
[Abstract]:Aim: to provide a model for the further study of the role of human bone marrow microenvironment in the development of human leukemia by reconstructing the human bone marrow microenvironment in immunodeficient mice. Methods: human platelet lysate (HPL), supplemented with 10%HPL and 10% fetal bovine serum (FBS) supplemented with 偽-MEM was used to culture mesenchymal stem cells derived from bone marrow by repeatedly freezing and thawing concentrated platelets. The effects of two additive components on the morphology, immunophenotype, multidirectional differentiation and proliferation of MSC were compared. The MSC cultured by HPL was inoculated into 尾-TCP scaffold and transplanted into the subcutaneous of the back of immunodeficient mice. After 8-12 weeks, the grafts were removed for HE staining to observe the ability of MSC to form bone and bone marrow structure in vivo. Results: M SC cultured with HPL and FBS showed long fusiform fibrous cell structure and multidirectional differentiation ability. There was no significant difference in the immunophenotype of M SC between the two systems, but the MSC cultured in human platelet lysate had stronger proliferative and osteogenic ability. MSC cultured with human platelet lysate has the ability to form bone-like structure in vivo. Conclusion: MSC cultured with HPL has stronger proliferative and osteogenic potential, and it can reconstruct human bone marrow microenvironment in NOD/SCID mice.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院血液學(xué)研究所血液病醫(yī)院實(shí)驗(yàn)血液學(xué)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81370599)
【分類號(hào)】：R593.3
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