大氣顆粒物與花粉聯(lián)合氧化損傷作用研究
發(fā)布時(shí)間：2018-09-19 11:15
【摘要】：目的空氣顆粒物(PM)可通過(guò)多種途徑導(dǎo)致花粉過(guò)敏癥的發(fā)病率增加或過(guò)敏癥的癥狀加重,其作用機(jī)制可能與氧化損傷的加重有關(guān)。本研究目的是了解不同大氣顆粒物和天津市夏、秋季的花粉等對(duì)中國(guó)倉(cāng)鼠肺細(xì)胞的毒性水平,研究大氣顆粒物的毒性損傷與花粉過(guò)敏反應(yīng)的相互作用,為可能的空氣污染物耦合健康損傷提供理論支持,同時(shí)為花粉過(guò)敏癥的預(yù)防和控制提供理論依據(jù)。方法對(duì)天津市煙道灰、燃煤飛灰和鍋底灰等顆粒物進(jìn)行采樣,并購(gòu)買(mǎi)天津市夏、秋季優(yōu)勢(shì)花粉豚草、梯牧草和楊樹(shù)花粉等作為實(shí)驗(yàn)樣品,選取中國(guó)倉(cāng)鼠肺細(xì)胞(CHL)作為實(shí)驗(yàn)對(duì)象。分別用顆粒物和花粉對(duì)細(xì)胞染毒,通過(guò)噻唑藍(lán)(MTT)比色法檢測(cè)各樣品不同濃度的細(xì)胞毒性。將花粉、顆粒物以及花粉和顆粒物的混合物對(duì)細(xì)胞染毒,分別檢測(cè)細(xì)胞和細(xì)胞培養(yǎng)基的氧化損傷指標(biāo),包括:丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和谷胱甘肽過(guò)氧化物酶(GSH-PX)活力等,比較花粉和顆粒物的混合物對(duì)細(xì)胞造成的氧化損傷是否高于單一污染物。結(jié)果1.顆粒物的細(xì)胞毒性:在實(shí)驗(yàn)濃度(12.5μg/ml~200μg/ml)范圍內(nèi),顆粒物對(duì)中國(guó)倉(cāng)鼠肺細(xì)胞(CHL)的生長(zhǎng)的抑制率隨染毒劑量增加而增加。當(dāng)顆粒物樣品的染毒劑量濃度為12.5μg/ml和25μg/ml時(shí),燃煤飛灰和鍋灰的細(xì)胞毒性相似(P0.05),而煙道灰的細(xì)胞毒性明顯高于以上兩種(P0.05)。隨染毒劑量濃度升高至50μg/ml和100μg/ml時(shí),燃煤飛灰和煙道灰表現(xiàn)出相似的細(xì)胞毒性(P0.05),而鍋灰的細(xì)胞毒性明顯小于上述兩種(P0.05)。當(dāng)染毒劑量濃度上升至200μg/ml時(shí),燃煤飛灰、鍋灰和煙道灰三種顆粒物對(duì)細(xì)胞生長(zhǎng)的機(jī)制作用未顯示出統(tǒng)計(jì)學(xué)差異(P0.05)。2.花粉的細(xì)胞毒性:在實(shí)驗(yàn)濃度(12.5μg/ml~200μg/ml)范圍內(nèi),花粉對(duì)中國(guó)倉(cāng)鼠肺細(xì)胞(CHL)的生長(zhǎng)抑制率隨染毒劑量增加而增加。在實(shí)驗(yàn)所用染毒濃度范圍內(nèi),相同濃度的楊樹(shù)和豚草表現(xiàn)出相似的細(xì)胞毒性(P0.05),當(dāng)花粉染毒劑量濃度為12.5μg/ml時(shí),梯牧草花粉的細(xì)胞濃度顯著低于豚草花粉和楊樹(shù)花粉(P0.05),而當(dāng)染毒劑量濃度為50μg/ml~200μg/ml時(shí),梯牧草花粉對(duì)細(xì)胞生長(zhǎng)的抑制率顯著高于豚草花粉和楊樹(shù)花粉(P0.05)。3.顆粒物和花粉的氧化損傷作用:顆粒物和混合物染毒組之間的丙二醛(MDA)含量對(duì)比空白組MDA含量均明顯增加(P0.05);對(duì)比豚草花粉單獨(dú)染毒或燃煤飛灰顆粒物單獨(dú)染毒,花粉和顆粒物混合物染毒組的MDA含量明顯升高(P0.05)。對(duì)比空白組總超氧化物歧化酶(T-SOD)活性,豚草花粉、燃煤飛灰顆粒物以及豚草花粉和燃煤飛灰顆粒物混合物染毒組的T-SOD活性顯著降低(P0.05);然而對(duì)比豚草花粉單獨(dú)染毒或燃煤飛灰顆粒物單獨(dú)染毒,豚草花粉和燃煤飛灰顆粒物混合物染毒組的T-SOD活性隨呈現(xiàn)下降趨勢(shì),但未顯示出統(tǒng)計(jì)學(xué)差異(P0.05)。對(duì)比空白組的谷胱甘肽過(guò)氧化物酶(GSH-PX)活性,豚草花粉、燃煤飛灰顆粒物以及豚草花粉和燃煤飛灰顆粒物混合物染毒組的GSH-PX活性均呈現(xiàn)下降趨勢(shì),然而僅有培養(yǎng)基中燃煤飛灰染毒組存在統(tǒng)計(jì)學(xué)差異(P0.05),其余各組均不存在統(tǒng)計(jì)學(xué)差異(P0.05);分別對(duì)比豚草花粉或燃煤飛灰單獨(dú)染毒,豚草花粉和燃煤飛灰顆粒物混合物染毒組中GSH-PX活性隨呈現(xiàn)下降趨勢(shì),但不存在統(tǒng)計(jì)學(xué)差異(P0.05)。說(shuō)明豚草花粉和燃煤飛灰顆粒物均可造成CHL細(xì)胞的氧化損傷,并且當(dāng)豚草花粉和燃煤飛灰顆粒物聯(lián)合作用是,其氧化損傷作用有增強(qiáng)的趨勢(shì)。結(jié)論顆粒物和花粉對(duì)細(xì)胞均表現(xiàn)出不同的細(xì)胞毒性。對(duì)比顆粒物或花粉的單一作用,顆粒物和花粉的聯(lián)合作用可增加對(duì)細(xì)胞的氧化損傷。
[Abstract]:Objective Air particulate matter (PM) can increase the incidence of pollen allergy or aggravate the symptoms of allergy through various ways. The mechanism may be related to the aggravation of oxidative damage. The interaction between toxic damage of particles and pollen allergic reaction provides theoretical support for the possible coupling health damage of air pollutants and provides theoretical basis for the prevention and control of pollen allergy. Chinese hamster lung cells (CHL) were selected as the experimental subjects. Cell toxicity was detected by MTT colorimetry. Pollen, particulate matter and the mixture of particulate matter and particulate matter were poisoned to cells, respectively. The content of MDA, SOD and GSH-PX were measured to compare the oxidative damage caused by the mixture of pollen and particulate matter. The inhibitory rate of particulate matter on the growth of Chinese hamster lung cells (CHL) increased with the increase of the dosage in the range of 12.5 to 200 ug/ml. When the dosage of particulate matter was 12.5 and 25 ug/ml, the cytotoxicity of Coal-fired Fly Ash and boiler ash was similar (P 0.05), but the cytotoxicity of flue ash was significantly higher than the above two (P 0.05). The cytotoxicity of fly ash and flue ash was similar (P 0.05) when the dosage was increased to 50 ug/ml and 100 ug/ml. The cytotoxicity of fly ash and flue ash was significantly lower than that of the above two (P 0.05). Pollen cytotoxicity: The growth inhibition rate of pollen on Chinese hamster lung cells (CHL) increased with the increase of exposure dose in the range of experimental concentration (12.5 to 200 ug/ml). Poplar and ragweed at the same concentration showed similar cytotoxicity in the range of experimental concentration (P 0.05). The cell concentration of T. terminalis pollen was significantly lower than that of ragweed pollen and poplar pollen (P 0.05) when the dose was 12.5 ug/ml, but the inhibition rate of T. terminalis pollen on cell growth was significantly higher than that of ragweed pollen and poplar pollen (P 0.05). 3. Oxidative damage of particles and pollen: particles Compared with the blank group, the content of MDA increased significantly (P 0.05); compared with the control group, the content of MDA increased significantly (P 0.05) in the pollen alone or in the fly ash particulate matter alone. Compared with the blank group, the total superoxide dismutase (T-SOD) activity in the pollen and particulate matter mixture group was significantly higher (P 0.05). T-SOD activity in the groups exposed to powder, fly ash particulate matter and the mixture of ragweed pollen and fly ash particulate matter decreased significantly (P The activity of glutathione peroxidase (GSH-PX) in the blank group, ragweed pollen, Coal-fired Fly ash particulate matter and the mixture of ragweed pollen and coal-fired fly ash particulate matter all showed a downward trend. However, only the Coal-fired Fly Ash group in the culture medium had a significant difference (P 0.05). There was no significant difference between the two groups (P 0.05). Compared with the single exposure to ragweed pollen or Coal-fired Fly ash, the GSH-PX activity of the mixture of ragweed pollen and coal-fired fly ash particles decreased, but there was no significant difference (P 0.05). It indicated that both ragweed pollen and coal-fired fly ash particles could cause oxidative damage to CHL cells, and there was no significant difference between the two groups. ConclusionParticles and pollens have different cytotoxicity to cells. Compared with the single effect of particulate matter or pollen, the combined effect of particulate matter and pollen can increase the oxidative damage to cells.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X513;R593.1
本文編號(hào)：2249972
[Abstract]:Objective Air particulate matter (PM) can increase the incidence of pollen allergy or aggravate the symptoms of allergy through various ways. The mechanism may be related to the aggravation of oxidative damage. The interaction between toxic damage of particles and pollen allergic reaction provides theoretical support for the possible coupling health damage of air pollutants and provides theoretical basis for the prevention and control of pollen allergy. Chinese hamster lung cells (CHL) were selected as the experimental subjects. Cell toxicity was detected by MTT colorimetry. Pollen, particulate matter and the mixture of particulate matter and particulate matter were poisoned to cells, respectively. The content of MDA, SOD and GSH-PX were measured to compare the oxidative damage caused by the mixture of pollen and particulate matter. The inhibitory rate of particulate matter on the growth of Chinese hamster lung cells (CHL) increased with the increase of the dosage in the range of 12.5 to 200 ug/ml. When the dosage of particulate matter was 12.5 and 25 ug/ml, the cytotoxicity of Coal-fired Fly Ash and boiler ash was similar (P 0.05), but the cytotoxicity of flue ash was significantly higher than the above two (P 0.05). The cytotoxicity of fly ash and flue ash was similar (P 0.05) when the dosage was increased to 50 ug/ml and 100 ug/ml. The cytotoxicity of fly ash and flue ash was significantly lower than that of the above two (P 0.05). Pollen cytotoxicity: The growth inhibition rate of pollen on Chinese hamster lung cells (CHL) increased with the increase of exposure dose in the range of experimental concentration (12.5 to 200 ug/ml). Poplar and ragweed at the same concentration showed similar cytotoxicity in the range of experimental concentration (P 0.05). The cell concentration of T. terminalis pollen was significantly lower than that of ragweed pollen and poplar pollen (P 0.05) when the dose was 12.5 ug/ml, but the inhibition rate of T. terminalis pollen on cell growth was significantly higher than that of ragweed pollen and poplar pollen (P 0.05). 3. Oxidative damage of particles and pollen: particles Compared with the blank group, the content of MDA increased significantly (P 0.05); compared with the control group, the content of MDA increased significantly (P 0.05) in the pollen alone or in the fly ash particulate matter alone. Compared with the blank group, the total superoxide dismutase (T-SOD) activity in the pollen and particulate matter mixture group was significantly higher (P 0.05). T-SOD activity in the groups exposed to powder, fly ash particulate matter and the mixture of ragweed pollen and fly ash particulate matter decreased significantly (P The activity of glutathione peroxidase (GSH-PX) in the blank group, ragweed pollen, Coal-fired Fly ash particulate matter and the mixture of ragweed pollen and coal-fired fly ash particulate matter all showed a downward trend. However, only the Coal-fired Fly Ash group in the culture medium had a significant difference (P 0.05). There was no significant difference between the two groups (P 0.05). Compared with the single exposure to ragweed pollen or Coal-fired Fly ash, the GSH-PX activity of the mixture of ragweed pollen and coal-fired fly ash particles decreased, but there was no significant difference (P 0.05). It indicated that both ragweed pollen and coal-fired fly ash particles could cause oxidative damage to CHL cells, and there was no significant difference between the two groups. ConclusionParticles and pollens have different cytotoxicity to cells. Compared with the single effect of particulate matter or pollen, the combined effect of particulate matter and pollen can increase the oxidative damage to cells.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X513;R593.1
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相關(guān)期刊論文 前2條
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2 張明慶;楊國(guó)棟;范振濤;張玲;;北京地區(qū)主要致敏花粉樹(shù)木花期的預(yù)報(bào)[J];環(huán)境與健康雜志;2008年03期
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