共信號(hào)分子CD28、ICOS、PD-1、CTLA-4在類風(fēng)濕關(guān)節(jié)炎的表達(dá)水平及意義
發(fā)布時(shí)間：2018-09-08 11:35
【摘要】：目的:為探討共信號(hào)分子CD28、ICOS、PD-1、CTLA-4在類風(fēng)濕關(guān)節(jié)炎的發(fā)病機(jī)制及意義,本實(shí)驗(yàn)通過(guò)流式細(xì)胞術(shù)檢測(cè)四種共信號(hào)分子在類風(fēng)濕關(guān)節(jié)炎患者外周血CD4+T細(xì)胞上的表達(dá)水平,并通過(guò)酶聯(lián)免疫吸附測(cè)定檢測(cè)sCD86、sICOS、sPD-1、sCTLA-4在類風(fēng)濕關(guān)節(jié)炎患者血漿中的表達(dá)水平,同時(shí)與DAS28評(píng)分進(jìn)行相關(guān)性分析。方法:(1)收集63例類風(fēng)濕關(guān)節(jié)炎患者(其中活動(dòng)期43例,穩(wěn)定期20例)和30例健康對(duì)照者的外周血標(biāo)本,通過(guò)流式細(xì)胞術(shù)檢測(cè)共信號(hào)分子CD28、ICOS、PD-1、CTLA-4在類風(fēng)濕關(guān)節(jié)炎患者活動(dòng)期、穩(wěn)定期及健康對(duì)照者外周血CD4+T細(xì)胞上的表達(dá)水平。(2)收集64例類風(fēng)濕關(guān)節(jié)炎患者(其中活動(dòng)期39例、穩(wěn)定期25例)和20例健康對(duì)照者的血漿,通過(guò)酶聯(lián)免疫吸附分別測(cè)定血漿中sCD86、sICOS、sPD-1、sCTLA-4在類風(fēng)濕關(guān)節(jié)炎患者活動(dòng)期、穩(wěn)定期及健康對(duì)照者的表達(dá)水平。(3)將上述共信號(hào)分子的表達(dá)水平與DAS28評(píng)分進(jìn)行Spearman相關(guān)性分析。結(jié)果:(1)流式細(xì)胞術(shù)分析結(jié)果顯示:在類風(fēng)濕關(guān)節(jié)炎患者中,活動(dòng)期組外周血CD4+T細(xì)胞上CD28、ICOS、PD-1的表達(dá)水平與健康對(duì)照組相比,差異均有統(tǒng)計(jì)學(xué)意義(P0.01;P0.05;P0.01),且均呈高表達(dá);活動(dòng)期組CD28、ICOS、PD-1的表達(dá)水平與穩(wěn)定期組相比,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05;P0.05;P0.05);活動(dòng)期組與健康對(duì)照組、穩(wěn)定期組相比,外周血CD4+T細(xì)胞上CTLA-4的表達(dá)水平差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05;P0.05)。(2)酶聯(lián)免疫吸附測(cè)定分析結(jié)果顯示:類風(fēng)濕關(guān)節(jié)炎患者活動(dòng)期組血漿中sCD86、sPD-1、sCTLA-4的表達(dá)水平與健康對(duì)照組相比差異均有統(tǒng)計(jì)學(xué)意義,均呈高表達(dá)(P0.05;P0.01;P0.01);活動(dòng)期組血漿中sICOS的表達(dá)水平與健康對(duì)照組相比差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);其中活動(dòng)期組血漿中sPD-1、sCTLA-4的表達(dá)水平較穩(wěn)定期組升高,差異有統(tǒng)計(jì)學(xué)意義(P0.01;P0.01),而sCD86、sICOS在活動(dòng)期組、穩(wěn)定期組血漿中的表達(dá)水平差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05;P0.05)。(3)類風(fēng)濕關(guān)節(jié)炎患者活動(dòng)期組外周血CD4+T細(xì)胞上ICOS的表達(dá)水平與DAS28評(píng)分呈正相關(guān),CD28、PD-1、CTLA-4的表達(dá)水平與DAS28評(píng)分無(wú)相關(guān)性;類風(fēng)濕關(guān)節(jié)炎患者活動(dòng)期組血漿中sPD-1、sCTLA-4的表達(dá)水平與DAS28評(píng)分呈正相關(guān),sCD86、sICOS的表達(dá)水平與DAS28評(píng)分無(wú)相關(guān)性。結(jié)論:共信號(hào)分子CD28、CD28的配體CD86、ICOS、PD-1和CTLA-4在類風(fēng)濕關(guān)節(jié)炎患者外周血CD4+T細(xì)胞上及血漿中表達(dá)異常,推測(cè)其在類風(fēng)濕關(guān)節(jié)炎的發(fā)病機(jī)制中可能發(fā)揮作用。其中sPD-1、sCTLA-4在RA血漿中的表達(dá)水平與DAS28評(píng)分呈正相關(guān),提示其與RA疾病活動(dòng)性相關(guān),可能成為疾病活動(dòng)監(jiān)測(cè)的指標(biāo)。
[Abstract]:Objective: to investigate the pathogenesis and significance of co-signal molecule CD28,ICOS,PD-1,CTLA-4 in rheumatoid arthritis (RA), the expression of four co-signaling molecules in peripheral blood CD4 T cells of patients with rheumatoid arthritis was detected by flow cytometry. The expression of sCD86,sICOS,sPD-1,sCTLA-4 in the plasma of patients with rheumatoid arthritis was detected by enzyme-linked immunosorbent assay (Elisa), and the correlation with DAS28 score was analyzed. Methods: (1) Peripheral blood samples of 63 patients with rheumatoid arthritis (43 active, 20 stable) and 30 healthy controls were collected. The co-signal molecule CD28,ICOS,PD-1,CTLA-4 was detected by flow cytometry in active stage of rheumatoid arthritis. The expression of CD4 T cells in peripheral blood of stable and healthy controls. (2) Plasma samples were collected from 64 patients with rheumatoid arthritis (39 active, 25 stable) and 20 healthy controls. The expression of sCD86,sICOS,sPD-1,sCTLA-4 in patients with rheumatoid arthritis (RA) was measured by Elisa. (3) the Spearman correlation analysis was performed between the expression of co-signal molecules and DAS28 score in patients with rheumatoid arthritis. Results: (1) the results of flow cytometry showed that the expression of CD28,ICOS,PD-1 on peripheral blood CD4 T cells in active group was significantly higher than that in healthy control group (P0.01, P0.05, P0.01). There was no significant difference in the expression of CD28,ICOS,PD-1 between the active group and the stable group (P0.05, P0.05, P0.05), while the expression of CD28,ICOS,PD-1 in the active phase group was higher than that in the healthy control group and the stable phase group, while in the active phase group, there was no significant difference between the two groups. There was no significant difference in the expression of CTLA-4 on peripheral blood CD4 T cells (P0.05P 0.05). (2). The results of enzyme-linked immunosorbent assay (Elisa) showed that the expression of sCD86,sPD-1,sCTLA-4 in plasma of active stage patients with rheumatoid arthritis was different from that of healthy controls. Are statistically significant, There was no significant difference in the expression of sICOS between the active group and the healthy control group (P0.05), and the expression level of sPD-1,sCTLA-4 in the active phase group was higher than that in the stable phase group (P 0.01), while the expression level of sCD86,sICOS in the active phase group was significantly higher than that in the stable phase group (P0.01), while in the active phase group, the expression level of sCD86,sICOS in the active phase group was significantly higher than that in the stable phase group (P0.01). There was no significant difference in plasma expression level between stable group and control group (P0.05P 0.05). (3). The expression of ICOS on peripheral blood CD4 T cells in active phase of rheumatoid arthritis patients was positively correlated with DAS28 score. There was no correlation between the expression level of CD28PD-1 CTLA-4 and DAS28 score. There was no correlation between the expression of sPD-1,sCTLA-4 in plasma and DAS28 score in active patients with rheumatoid arthritis. There was no correlation between the expression level of sCD86 and DAS28 score. Conclusion: the expression of CD86,ICOS,PD-1 and CTLA-4 in peripheral blood CD4 T cells and plasma of patients with rheumatoid arthritis (RA) is abnormal, which suggests that CD86,ICOS,PD-1 and CTLA-4 may play a role in the pathogenesis of rheumatoid arthritis. The expression of sPD-1,sCTLA-4 in the plasma of RA was positively correlated with the DAS28 score, which suggested that the expression level of sPD-1,sCTLA-4 was correlated with the disease activity of RA, and might become an indicator of disease activity monitoring.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R593.22
本文編號(hào)：2230399
[Abstract]:Objective: to investigate the pathogenesis and significance of co-signal molecule CD28,ICOS,PD-1,CTLA-4 in rheumatoid arthritis (RA), the expression of four co-signaling molecules in peripheral blood CD4 T cells of patients with rheumatoid arthritis was detected by flow cytometry. The expression of sCD86,sICOS,sPD-1,sCTLA-4 in the plasma of patients with rheumatoid arthritis was detected by enzyme-linked immunosorbent assay (Elisa), and the correlation with DAS28 score was analyzed. Methods: (1) Peripheral blood samples of 63 patients with rheumatoid arthritis (43 active, 20 stable) and 30 healthy controls were collected. The co-signal molecule CD28,ICOS,PD-1,CTLA-4 was detected by flow cytometry in active stage of rheumatoid arthritis. The expression of CD4 T cells in peripheral blood of stable and healthy controls. (2) Plasma samples were collected from 64 patients with rheumatoid arthritis (39 active, 25 stable) and 20 healthy controls. The expression of sCD86,sICOS,sPD-1,sCTLA-4 in patients with rheumatoid arthritis (RA) was measured by Elisa. (3) the Spearman correlation analysis was performed between the expression of co-signal molecules and DAS28 score in patients with rheumatoid arthritis. Results: (1) the results of flow cytometry showed that the expression of CD28,ICOS,PD-1 on peripheral blood CD4 T cells in active group was significantly higher than that in healthy control group (P0.01, P0.05, P0.01). There was no significant difference in the expression of CD28,ICOS,PD-1 between the active group and the stable group (P0.05, P0.05, P0.05), while the expression of CD28,ICOS,PD-1 in the active phase group was higher than that in the healthy control group and the stable phase group, while in the active phase group, there was no significant difference between the two groups. There was no significant difference in the expression of CTLA-4 on peripheral blood CD4 T cells (P0.05P 0.05). (2). The results of enzyme-linked immunosorbent assay (Elisa) showed that the expression of sCD86,sPD-1,sCTLA-4 in plasma of active stage patients with rheumatoid arthritis was different from that of healthy controls. Are statistically significant, There was no significant difference in the expression of sICOS between the active group and the healthy control group (P0.05), and the expression level of sPD-1,sCTLA-4 in the active phase group was higher than that in the stable phase group (P 0.01), while the expression level of sCD86,sICOS in the active phase group was significantly higher than that in the stable phase group (P0.01), while in the active phase group, the expression level of sCD86,sICOS in the active phase group was significantly higher than that in the stable phase group (P0.01). There was no significant difference in plasma expression level between stable group and control group (P0.05P 0.05). (3). The expression of ICOS on peripheral blood CD4 T cells in active phase of rheumatoid arthritis patients was positively correlated with DAS28 score. There was no correlation between the expression level of CD28PD-1 CTLA-4 and DAS28 score. There was no correlation between the expression of sPD-1,sCTLA-4 in plasma and DAS28 score in active patients with rheumatoid arthritis. There was no correlation between the expression level of sCD86 and DAS28 score. Conclusion: the expression of CD86,ICOS,PD-1 and CTLA-4 in peripheral blood CD4 T cells and plasma of patients with rheumatoid arthritis (RA) is abnormal, which suggests that CD86,ICOS,PD-1 and CTLA-4 may play a role in the pathogenesis of rheumatoid arthritis. The expression of sPD-1,sCTLA-4 in the plasma of RA was positively correlated with the DAS28 score, which suggested that the expression level of sPD-1,sCTLA-4 was correlated with the disease activity of RA, and might become an indicator of disease activity monitoring.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R593.22
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